CD21 Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: CD21 Antibody [NBP2-38895] - Analysis in human lymph node and endometrium tissues. Corresponding CR2 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: CD21 Antibody [NBP2-38895] -Staining of human lymph node shows strong membranous positivity in germinal center cells.
Independent Antibodies: Immunohistochemistry-Paraffin: CD21 Antibody [NBP2-38895] - Staining of human endometrium, kidney, lymph node and tonsil using Anti-CD21 antibody NBP2-38895 (A) shows similar protein ...read more
Western Blot: CD21 Antibody [NBP2-38895] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver tissue. ...read more
Orthogonal Strategies: Immunohistochemistry-Paraffin: CD21 Antibody [NBP2-38895] - Staining of human endometrium shows no positivity in glandular cells as expected.
Independent Antibodies: Immunohistochemistry-Paraffin: CD21 Antibody [NBP2-38895] - Staining of human lymph node shows strong membranous positivity in germinal center cells.
Immunohistochemistry-Paraffin: CD21 Antibody [NBP2-38895] - Staining of human tonsil shows strong membranous positivity in germinal center cells.
Immunohistochemistry-Paraffin: CD21 Antibody [NBP2-38895] - Staining of human kidney shows very weak membranous positivity in cells in tubules as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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CD21 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: VIRYSCSGTFRLIGEKSLLCITKDKVDGTWDKPAPKCEYFNKYSSCPEPIVPGGYKIRGSTPYRHGDSVTFACKTNFSMNGNKS
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CR2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
CD21 Protein (NBP2-38895PEP)
Publications
Read Publication using
NBP2-38895 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CD21 Antibody

  • C3DR
  • C3DRSLEB9
  • CD21 antigen
  • CD21
  • Complement C3d receptor
  • complement component (3d/Epstein Barr virus) receptor 2
  • complement receptor type 2
  • CR2
  • EBV receptor
  • Epstein-Barr virus receptor

Background

CD21 (complement receptor 2, CR2) binds C3 complement fragments, especially its breakdown fragments, which remain covalently attached to complement activating surfaces or antigen. CD21 has important roles in uptake and retention of immunocomplexes, survival of memory B cells and in development and maintenance of the humoral response to T-dependent antigens. CD21 also serves as a key receptor for Epstein-Barr virus binding and is involved in targeting prions to folicular dendritic cells and expediting neuroinvasion following peripheral exposure to prions. A soluble form of the CD21 (sCD21) is shed from the lymphocyte surface and retains its ability to bind respective ligands.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD21 Antibody (NBP2-38895)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IF/IHC.


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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Research Areas for CD21 Antibody (NBP2-38895)

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Blogs on CD21.

How To Identify B Cell Subsets Using Flow Cytometry
By Victoria OsinskiUsing Flow Cytometry to Identify B Cell SubsetsIdentifying cellular subsets by flow cytometry requires careful and thorough planning in order to ensure the correct subset of cells are identified...  Read full blog post.

CD19: An Undoubted Biomarker for B Cells
CD19 is a cell surface protein member of the large immunoglobulin superfamily that complexes with CD21, CD81, and CD225 in the membrane of mature B-cells. A major function of CD19 is to assemble with the antigen receptor of B-lymphocytes to decrease t...  Read full blog post.

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Bioinformatics

Gene Symbol CR2
Uniprot