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CCR8 Antibody (9N1Y1)

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Western Blot: CCR8 Antibody (9N1Y1) [NBP3-16370] - Western blot analysis of extracts of various cell lines, using CCR8 Rabbit mAb (NBP3-16370) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Western Blot: CCR8 Antibody (9N1Y1) [NBP3-16370] - Western blot analysis of extracts of various cell lines, using CCR8 Rabbit mAb (NBP3-16370) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at ...read more
Immunocytochemistry/ Immunofluorescence: CCR8 Antibody (9N1Y1) [NBP3-16370] - Immunofluorescence analysis of Jurkat cells using CCR8 Rabbit mAb (NBP3-16370) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
9N1Y1
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

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CCR8 Antibody (9N1Y1) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 250-355 of human CCR8 (P51685). FWVPFNVVLFLTSLHSMHILDGCSISQQLTYATHVTEIISFTHCCVNPVIYAFVGEKFKKHLSEIFQKSCSQIFNYLGRQMPRESCEKSSSCQQHSSRSSSVDYIL
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
CCR8
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Western Blot 1:500 - 1:2000
Theoretical MW
41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for CCR8 Antibody (9N1Y1)

  • CC chemokine receptor 8
  • C-C CKR-8
  • CC-chemokine receptor chemr1
  • CC-CKR-8
  • CCR8
  • CCR-8
  • CD198
  • CDw198 antigen
  • CDw198
  • chemokine (C-C motif) receptor 8
  • chemokine (C-C) receptor 8
  • chemokine (C-C) receptor-like 2
  • Chemokine receptor-like 1
  • ChemR1
  • CKRL1
  • CKR-L1
  • CKRL1MGC129966
  • CMKBR8MGC129973
  • CMKBRL2C-C chemokine receptor type 8
  • CY6
  • GPRCL1
  • GPRCY6
  • GPR-CY6
  • TER-1
  • TER1CC chemokine receptor CHEMR1

Background

CKR8/CCR8, or C-C chemokine receptor type 8, is a G protein-coupled, seven-transmembrane domain receptor protein. Chemokines are key molecules in directing leukocyte migration toward sites of inflammation. The human C-C chemokine I-309 is a monocyte chemoattractant and inhibits apoptosis in thymic cell lines. It has been found that CKR8/CCR8 is a receptor for chemokines SCYA1/I-309, SCYA4/MIP-1-beta, and SCYA17/TARC (1-3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CCR8 Antibody
(9N1Y1)
NBP3-16370
CCR8 Antibody (9N1Y1)
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CCR8