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CCR10/GPR2 Overexpression Lysate

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Western Blot: GPR2/CCR10 Overexpression Lysate (Adult Normal) [NBL1-08888] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GPR2/CCR10.
Western Blot: GPR2/CCR10 Overexpression Lysate (Adult Normal) [NBL1-08888] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GPR2/CCR10.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

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CCR10/GPR2 Overexpression Lysate Summary

Description

CCR10/GPR2 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC222924

Accession#: NM_016602

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
CCR10

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
38.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for CCR10/GPR2 Overexpression Lysate

  • CC chemokine receptor 10
  • C-C chemokine receptor type 10
  • C-C CKR-10
  • CC-CKR-10
  • CCR10
  • CCR-10
  • chemokine (C-C motif) receptor 10
  • G protein-coupled receptor 2
  • GPR2
  • GPR2G-protein coupled receptor 2

Background

Receptors for the C-C chemokine family include CCR-1, CCR-2A, CCR-3, CCR-4, CCR-5 and the Duffy blood group antigen. The C-C receptors are important in the function of T cell chemotaxis and migration of phagocytic cells to sites of inflammation. CCR10 in particular is the receptor for the skin-associated CC chemokine CCL27 (CTACK) as well as the novel chemokine CCL28 which displays chemotactic activity for resting CD4+ and CD8+ T cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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CCR10/GPR2
Overexpression Lysate
NBL1-08888
CCR10/GPR2 Overexpression Lysate
Species: Hu
Applications: WB

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Bioinformatics

Gene Symbol CCR10