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CASK Antibody

Images

 
Western Blot: CASK Antibody [NBP2-15710] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CASK antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody ...read more
Immunocytochemistry/ Immunofluorescence: CASK Antibody [NBP2-15710] - Immunofluorescence analysis of paraformaldehyde-fixed A431, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: CASK Antibody [NBP2-15710] - Paraffin-embedded mouse lung. CASK antibody diluted at 1:500.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, XpSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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CASK Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human CASK. The exact sequence is proprietary.
Localization
Nucleus; Cytoplasm; Cell membrane; Peripheral membrane protein
Predicted Species
Bovine (91%), Xenopus (95%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CASK
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
105 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (95%), Rhesus Monkey (86%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for CASK Antibody

  • calcium/calmodulin-dependent serine protein kinase (MAGUK family)
  • calcium/calmodulin-dependent serine protein kinase membrane-associatedguanylate kinase
  • Calcium/calmodulin-dependent serine protein kinase
  • CAMGUK
  • CMG
  • EC 2.7.11
  • EC 2.7.11.1
  • FGS4CAGH39
  • FLJ22219
  • hCASK
  • LIN2FLJ31914
  • MICPCH
  • peripheral plasma membrane protein CASK
  • Protein lin-2 homolog
  • TNRC8
  • trinucleotide repeat containing 8

Background

The CASK gene encodes a calcium/calmodulin-dependent serine protein kinase that is a member of the membrane-associated guanylate kinase (MAGUK) protein family. MAGUKs are scaffolding proteins associated with intercellular junctions (Atasoy et al., 2007 [PubMed 17287346]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional CASK Products

Array NBP2-15710

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Bioinformatics

Gene Symbol CASK
Uniprot