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CARD11/CARMA1 Antibody

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Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, A20 mouse B cell lymphoma cell line, and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat CARD11/CARMA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4839) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CARD11/CARMA1 at approximately 133 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, A20 mouse B cell lymphoma cell line, and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat CARD11/CARMA1 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF4839-SP
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CARD11/CARMA1 Antibody Summary

Immunogen
E. coli-derived recombinant human CARD11
Lys263-Ser442
Accession # Q9BXL7
Specificity
Detects endogenous human, mouse, and rat CARMA1 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CARD11
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CARD11/CARMA1 Antibody

  • BIMP3
  • BIMP3card-maguk protein 1
  • CARD11
  • CARD-containing MAGUK protein 1
  • Carma 1
  • CARMA1
  • CARMA1bcl10-interacting maguk protein 3
  • caspase recruitment domain family, member 11
  • caspase recruitment domain-containing protein 11
  • MGC133069

Background

CARMA1 (CARD-MAGUK1; also CARD11) is a 133 kDa member of the MAGUK family of proteins. It is a cytoplasmic promoter of Bcl10 phosphorylation, and as such, regulates the effectiveness of NF-kappa B signaling. Human CARMA1 is 1154 amino acids (aa) in length and contains an N-terminal CARD region, two coiled-coil (CC) domains (aa 123‑250 and 295‑442), and a PZD, SH3 and MAGUK domain (aa 996‑1133). The CC1 and MAGUK domains position the molecule, while CC1 and CC2 mediate a necessary dimerization/oligomerization. There is one potential alternate start site seven aa upstream from the standard start site. Over aa 263-442, human CARMA1 is 98% aa identical to both mouse and canine CARMA1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CARD11/CARMA1
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CARD11/CARMA1 Antibody
Species: Hu, Mu, Rt
Applications: WB

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Bioinformatics

Gene Symbol CARD11
Uniprot