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PKC theta Antibody (453416) [Unconjugated]

 
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Jurkat human acute T cell leukemia cell line was stained with Human PKC theta Monoclonal Antibody (Catalog # MAB4368, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.
Jurkat human acute T cell leukemia cell line was stained with Human PKC theta Monoclonal Antibody (Catalog # MAB4368, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by ...read more
Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 2 µg/mL of Human PKC theta Monoclonal Antibody (Catalog # MAB4368) followed by HRP-conjugated Anti-Mouse IgG ...read more
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for PKC theta at approximately 82 kDa (as indicated) using 5 µg/mL of Mouse ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, CyTOF-ready, Flow
Clone
453416
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC4368U-100UG
Alexa Fluor 405
IC4368V-100UG
Alexa Fluor 488
IC4368G-100UG
Alexa Fluor 532
FAB4368X
Alexa Fluor 594
IC4368T-100UG
Alexa Fluor 647
IC4368R-100UG
Alexa Fluor 700
IC4368N-100UG
Alexa Fluor 750
IC4368S-100UG
Allophycocyanin
FAB4368A
Biotin
FAB4368B
CoraFluor 1
FAB4368CL1
DyLight 350
FAB4368D
DyLight 405
FAB4368E
DyLight 488
FAB4368K
DyLight 550
FAB4368L
DyLight 594
FAB4368M
DyLight 650
FAB4368W
DyLight 680
FAB4368Y
DyLight 755
FAB4368Z
FITC
FAB4368F
HRP
FAB4368H
Janelia Fluor 525
FAB4368JF525
Janelia Fluor 549
FAB4368I
Janelia Fluor 646
FAB4368J
Janelia Fluor 669
FAB4368JF669
PE
FAB4368P
PerCP
FAB4368C
mFluor Violet 450 SE
FAB4368MFV450
mFluor Violet 500 SE
FAB4368MFV500
mFluor Violet 610 SE
FAB4368MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB4368-SP
Carrier Free
IC4368G-100UG
Carrier Free
IC4368V-100UG
Carrier Free
IC4368U-100UG
Carrier Free
IC4368T-100UG
Carrier Free
IC4368S-100UG
Carrier Free
IC4368R-100UG
Carrier Free
IC4368N-100UG
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PKC theta Antibody (453416) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human PKC theta
His580-Ser706
Accession # Q04759
Specificity
Detects human PKC theta . This antibody also detects recombinant human PKC theta , but does not detect any other recombinant human PKC family member.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
PRKCQ
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Simple Western 5 ug/mL
  • Western Blot 2 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PKC theta Antibody (453416) [Unconjugated]

  • EC 2.7.11
  • EC 2.7.11.13
  • MGC126514
  • MGC141919
  • nPKC-theta
  • PKC theta
  • PRKCQ
  • PRKCT
  • protein kinase C theta type
  • protein kinase C, theta

Background

PKC theta (PRKCQ) is a calcium-independent phospholipid-dependent serine and threonine kinase that belongs to the novel PKC subfamily. PKC theta plays a critical role in several aspects of T cell biology, and knockout mice develop autoimmune disease. PKC theta functions through the activation of NF kappa B and AP1 transcription factors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PRKCQ
Uniprot