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Vav-1 [p Tyr160] Antibody (751411) [Unconjugated]

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Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 5 minutes and 10 µg/mL Mouse Anti-Human CD3e Monoclonal Antibody (Catalog # MAB100) for 15 minutes. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Phospho-Vav-1 (Y160) Monoclonal Antibody (Catalog # MAB37861) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Phospho-Vav-1 (Y160) at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 5 minutes and 10 µg/mL Mouse Anti-Human CD3e Monoclonal Antibody (Catalog # MAB100) ...read more
Vav-1 phosphorylated at Y160 was detected in immersion fixed Jurkat human acute T cell leukemia cell line treated with (upper panel) or without (lower panel) Pervanadate using Mouse Anti-Human Phospho-Vav-1 (Y160) ...read more
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 5 minutes, loaded at 0.2 mg/mL. A specific band was detected for Human ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clone
751411
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB37861-SP
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Vav-1 [p Tyr160] Antibody (751411) [Unconjugated] Summary

Immunogen
Phosphopeptide containing the human Vav-1 Y160 site
Modification
p Tyr160
Specificity
Detects human VAV-1 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
VAV1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Simple Western 1 ug/mL
  • Western Blot 0.1 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Publications
Read Publication using
MAB37861 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Vav-1 [p Tyr160] Antibody (751411) [Unconjugated]

  • oncogene vav
  • proto-oncogene vav
  • vav 1 guanine nucleotide exchange factor
  • vav proto-oncogene
  • Vav1
  • Vav-1
  • vav-T
  • VAVvav 1 oncogene

Background

The Vav-1 proto-oncogene is a 95 kDa guanine nucleotide exchange factor (GEF) for the low molecular weight GTPases of the Rho/Rac family. Vav-1 is a cytosolic protein that is primarily expressed in hematopoietic cells and plays an essential role in the proliferation and activation of T and B cells. It promotes intracellular signaling by its multiple protein binding motifs. Vav-1 is phosphorylated on Y160 after cell adhesion via integrin avb3. This phosphorylation is necessary for adhesion‑mediated Rho activation and association between avb3 and Vav-1. The peptide immunogen represents a sequence that is conserved between human, mouse and rat Vav-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Vav-1 [p Tyr160]
Antibody (751411) [Un ...
MAB37861
Vav-1 [p Tyr160] Antibody (751411) [Unconjugated]
Species: Hu
Applications: WB, Simple Western, ICC/IF

Publications for Vav-1 Antibody (MAB37861)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Western Blot.


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Bioinformatics

Gene Symbol VAV1