Home » SLP-76/LCP2 » SLP-76 Antibodies » SLP-76/LCP2 [p Tyr145] Antibody (776503) [Unconjugated]
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SLP-76/LCP2 [p Tyr145] Antibody (776503) [Unconjugated]

 
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Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 5 minutes. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Phospho-SLP-76/LCP2 (Y145) Monoclonal Antibody (Catalog # MAB7474) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Phospho-SLP-76/LCP2 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 5 minutes. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human ...read more
SLP-76/LCP2 phosphorylated at Y145 was detected in immersion fixed Jurkat human acute T cell leukemia cells treated with (upper panel) or without (lower panel) Pervanadate using Mouse Anti-Human Phospho-SLP-76/LCP2 ...read more
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 5 minutes, loaded at 0.2 mg/mL. A specific band was detected for ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clone
776503
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB7474-SP
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SLP-76/LCP2 [p Tyr145] Antibody (776503) [Unconjugated] Summary

Immunogen
Phosphopeptide containing the human SLP-76/LCP2 Y145 site
Accession # Q13094
Modification
p Tyr145
Specificity
Detects human Phospho-SLP-76/LCP2 in direct ELISAs.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
LCP2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Simple Western 1 ug/mL
  • Western Blot 0.1 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SLP-76/LCP2 [p Tyr145] Antibody (776503) [Unconjugated]

  • LCP2
  • lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of76kDa)
  • lymphocyte cytosolic protein 2 (SH2 domain-containing leukocyte protein of76kD)
  • lymphocyte cytosolic protein 2
  • SH2 domain-containing leukocyte protein of 76 kDa
  • SH2 domain-containing leukocyte protein of 76kD
  • SLP-76 tyrosine phosphoprotein
  • SLP76
  • SLP-76
  • SLP7676 kDa tyrosine phosphoprotein

Background

SLP-76 is a 533 amino acid (aa), 76 kDa tyrosine phosphorylated intracellular docking protein with a single SH2 recognition domain. It is expressed in T cells, platelets, neutrophils, NK and mast cells, and is required for progression of T cells to double positive stage in the thymus. Tyrosine phosphorylation is required for interaction with the signaling proteins VAV, NCK, PLC gamma , ITK, and ZAP70. SLP-76 may be phosphorylated by ZAP70 leading to NF-AT and IL2 gene activation. Human SLP-76 shares 83% and 85% aa sequence identity with mouse and rat SLP-76, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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SLP-76/LCP2 [p
Tyr145] Antibody (776 ...
MAB7474
SLP-76/LCP2 [p Tyr145] Antibody (776503) [Unconjugated]
Species: Hu
Applications: WB, Simple Western, ICC/IF

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Product General Protocols

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Bioinformatics

Gene Symbol LCP2
Uniprot