![Knockdown Validated: BRCC3 Antibody [NBP1-76831] - Immunoblot analysis of the effect of JIB-04 on Tat protein levels in 2D10 cells depleted of SHMT2 or BRCC36. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.ppat.1007071) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BRCC3-Antibody-Knockdown-Validated-NBP1-76831-img0008.jpg "Knockdown Validated: BRCC3 Antibody [NBP1-76831] - Immunoblot analysis of the effect of JIB-04 on Tat protein levels in 2D10 cells depleted of SHMT2 or BRCC36. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.ppat.1007071) licensed under a CC-BY license.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC, KD |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Antibody was raised against a 18 amino acid synthetic peptide from near the amino terminus of human BRCC36. The immunogen is located within amino acids 20 - 70 of BRCC36. Amino Acid Squence: CIGELNDDTRSDSKFAY |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | BRCC3 |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 35 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Control Peptide |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Peptide affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: BRCC3 Antibody [NBP1-76831] - Representative IF images showing BRCC3 signal in LT versus ST and MPPs. Scale bar 5 um. m Quantitation of BRCC3 IF signal from l in Msi2 WT and Msi2 K/O. n = 258; 263; 216 and 295 cells for LT Msi2 WT; KO; ST, MPPs Msi2 WT and KO, respectively. Data as mean +/- SEM. Unpaired Student t test, ****p < 0.0001. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-020-15814-8) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BRCC3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76831-img0007.jpg "Immunocytochemistry/Immunofluorescence: BRCC3 Antibody [NBP1-76831] - Representative IF images showing BRCC3 signal in LT versus ST and MPPs. Scale bar 5 um. m Quantitation of BRCC3 IF signal from l in Msi2 WT and Msi2 K/O. n = 258; 263; 216 and 295 cells for LT Msi2 WT; KO; ST, MPPs Msi2 WT and KO, respectively. Data as mean +/- SEM. Unpaired Student t test, ****p < 0.0001. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-020-15814-8) licensed under a CC-BY license.")
![Western Blot: BRCC3 Antibody [NBP1-76831] - Analysis of BRCC36 in MCF7 cell lysate with BRCC36 antibody at (A) 0.5 and (B) 1 ug/mL.](http://images.novusbio.com/fullsize/BRCC3-Antibody-Western-Blot-NBP1-76831-img0006.jpg "Western Blot: BRCC3 Antibody [NBP1-76831] - Analysis of BRCC36 in MCF7 cell lysate with BRCC36 antibody at (A) 0.5 and (B) 1 ug/mL.")
![Immunohistochemistry-Paraffin: BRCC3 Antibody [NBP1-76831] - Human brain tissue with BRCC3 antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/BRCC3-Antibody-Immunohistochemistry-Paraffin-NBP1-76831-img0001.jpg "Immunohistochemistry-Paraffin: BRCC3 Antibody [NBP1-76831] - Human brain tissue with BRCC3 antibody at 2.5 ug/ml.")
![Immunocytochemistry/Immunofluorescence: BRCC3 Antibody [NBP1-76831] - Immunofluorescence of BRCC36 in Human Brain cells with BRCC36 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/BRCC3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76831-img0003.jpg "Immunocytochemistry/Immunofluorescence: BRCC3 Antibody [NBP1-76831] - Immunofluorescence of BRCC36 in Human Brain cells with BRCC36 antibody at 20 ug/mL.")
![Western Blot: BRCC3 Antibody - BSA Free [NBP1-76831] - SHMT2 & the BRCC36/BRISC deubiquitinase controls Tat K63Ub & destruction by autophagy.(A) Immunoblot analysis of Tat & GFP protein levels in 2D10 cells depleted of SHMT2 or Cyclin T1, as indicated. TAF4 served as loading control. (B) Immunoblot analysis of Tat protein expression in 2D10 cells depleted of BRCC36 or Cyclin T1. NF-kappa B2 protein levels were monitored to assess any change in T cell signaling. TAF4 served as loading control. (C) Immunoblot analysis of Tat expression in Tet-on-Tat-off HeLa cells depleted of SHMT1, SHMT2 or BRCC36, as indicated above each lane. TAF4 served as loading control. (D) Top, immunoblot analysis of the effects of overexpression of Flag-SHMT1 & FH-BRCC36 proteins on Tat protein levels. TAF4 served as loading control. Bottom, Dual:Luc reporter activity of the HIV-Luc reporter. Plasmids expressing the Flag-vector, Flag-SHMT1 & FH-BRCC36 proteins were tested at 0 ng (blue bar), 20 ng (purple bar), 100 ng (yellow bar) & 500 ng (light blue bar), as indicated. (E) Analysis of FLAG-Tat-101 immunoprecipitates from lysates of BRCC36- or SHMT1- knockdown cells. FLAG-Tat-101 proteins were labelled with HA-tagged ubiquitin, either wild-type Ub (WT), or ubiquitin mutants that selectively support only K63 or K48 ubiquitylation, & Tat proteins were monitored using anti-HA antisera. (F) Immunoblot analysis of the effect of JIB-04 on Tat protein levels in 2D10 cells depleted of SHMT2 or BRCC36. (G) The effect of JIB-04 on wildtype or Tat delta K (+K41) STREP-tagged Tat proteins was shown by immunoblot. TAF4 served as loading control. (H) Model of the role of SHMT2 & BRCC36 in the release of Tat-K63Ub from destruction through chaperone-mediated autophagy or SQSMT1/p62-dependent macroautophagy. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.ppat.1007071), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-76831_rabbit-polyclonal-brcc3-antibody-310202416163777.jpg "Western Blot: BRCC3 Antibody - BSA Free [NBP1-76831] - SHMT2 & the BRCC36/BRISC deubiquitinase controls Tat K63Ub & destruction by autophagy.(A) Immunoblot analysis of Tat & GFP protein levels in 2D10 cells depleted of SHMT2 or Cyclin T1, as indicated. TAF4 served as loading control. (B) Immunoblot analysis of Tat protein expression in 2D10 cells depleted of BRCC36 or Cyclin T1. NF-kappa B2 protein levels were monitored to assess any change in T cell signaling. TAF4 served as loading control. (C) Immunoblot analysis of Tat expression in Tet-on-Tat-off HeLa cells depleted of SHMT1, SHMT2 or BRCC36, as indicated above each lane. TAF4 served as loading control. (D) Top, immunoblot analysis of the effects of overexpression of Flag-SHMT1 & FH-BRCC36 proteins on Tat protein levels. TAF4 served as loading control. Bottom, Dual:Luc reporter activity of the HIV-Luc reporter. Plasmids expressing the Flag-vector, Flag-SHMT1 & FH-BRCC36 proteins were tested at 0 ng (blue bar), 20 ng (purple bar), 100 ng (yellow bar) & 500 ng (light blue bar), as indicated. (E) Analysis of FLAG-Tat-101 immunoprecipitates from lysates of BRCC36- or SHMT1- knockdown cells. FLAG-Tat-101 proteins were labelled with HA-tagged ubiquitin, either wild-type Ub (WT), or ubiquitin mutants that selectively support only K63 or K48 ubiquitylation, & Tat proteins were monitored using anti-HA antisera. (F) Immunoblot analysis of the effect of JIB-04 on Tat protein levels in 2D10 cells depleted of SHMT2 or BRCC36. (G) The effect of JIB-04 on wildtype or Tat delta K (+K41) STREP-tagged Tat proteins was shown by immunoblot. TAF4 served as loading control. (H) Model of the role of SHMT2 & BRCC36 in the release of Tat-K63Ub from destruction through chaperone-mediated autophagy or SQSMT1/p62-dependent macroautophagy. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.ppat.1007071), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: BRCC3 Antibody - BSA Free [NBP1-76831] - SHMT2 & the BRCC36/BRISC deubiquitinase controls Tat K63Ub & destruction by autophagy.(A) Immunoblot analysis of Tat & GFP protein levels in 2D10 cells depleted of SHMT2 or Cyclin T1, as indicated. TAF4 served as loading control. (B) Immunoblot analysis of Tat protein expression in 2D10 cells depleted of BRCC36 or Cyclin T1. NF-kappa B2 protein levels were monitored to assess any change in T cell signaling. TAF4 served as loading control. (C) Immunoblot analysis of Tat expression in Tet-on-Tat-off HeLa cells depleted of SHMT1, SHMT2 or BRCC36, as indicated above each lane. TAF4 served as loading control. (D) Top, immunoblot analysis of the effects of overexpression of Flag-SHMT1 & FH-BRCC36 proteins on Tat protein levels. TAF4 served as loading control. Bottom, Dual:Luc reporter activity of the HIV-Luc reporter. Plasmids expressing the Flag-vector, Flag-SHMT1 & FH-BRCC36 proteins were tested at 0 ng (blue bar), 20 ng (purple bar), 100 ng (yellow bar) & 500 ng (light blue bar), as indicated. (E) Analysis of FLAG-Tat-101 immunoprecipitates from lysates of BRCC36- or SHMT1- knockdown cells. FLAG-Tat-101 proteins were labelled with HA-tagged ubiquitin, either wild-type Ub (WT), or ubiquitin mutants that selectively support only K63 or K48 ubiquitylation, & Tat proteins were monitored using anti-HA antisera. (F) Immunoblot analysis of the effect of JIB-04 on Tat protein levels in 2D10 cells depleted of SHMT2 or BRCC36. (G) The effect of JIB-04 on wildtype or Tat delta K (+K41) STREP-tagged Tat proteins was shown by immunoblot. TAF4 served as loading control. (H) Model of the role of SHMT2 & BRCC36 in the release of Tat-K63Ub from destruction through chaperone-mediated autophagy or SQSMT1/p62-dependent macroautophagy. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.ppat.1007071), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-76831_rabbit-polyclonal-brcc3-antibody-31020241616070.jpg "Western Blot: BRCC3 Antibody - BSA Free [NBP1-76831] - SHMT2 & the BRCC36/BRISC deubiquitinase controls Tat K63Ub & destruction by autophagy.(A) Immunoblot analysis of Tat & GFP protein levels in 2D10 cells depleted of SHMT2 or Cyclin T1, as indicated. TAF4 served as loading control. (B) Immunoblot analysis of Tat protein expression in 2D10 cells depleted of BRCC36 or Cyclin T1. NF-kappa B2 protein levels were monitored to assess any change in T cell signaling. TAF4 served as loading control. (C) Immunoblot analysis of Tat expression in Tet-on-Tat-off HeLa cells depleted of SHMT1, SHMT2 or BRCC36, as indicated above each lane. TAF4 served as loading control. (D) Top, immunoblot analysis of the effects of overexpression of Flag-SHMT1 & FH-BRCC36 proteins on Tat protein levels. TAF4 served as loading control. Bottom, Dual:Luc reporter activity of the HIV-Luc reporter. Plasmids expressing the Flag-vector, Flag-SHMT1 & FH-BRCC36 proteins were tested at 0 ng (blue bar), 20 ng (purple bar), 100 ng (yellow bar) & 500 ng (light blue bar), as indicated. (E) Analysis of FLAG-Tat-101 immunoprecipitates from lysates of BRCC36- or SHMT1- knockdown cells. FLAG-Tat-101 proteins were labelled with HA-tagged ubiquitin, either wild-type Ub (WT), or ubiquitin mutants that selectively support only K63 or K48 ubiquitylation, & Tat proteins were monitored using anti-HA antisera. (F) Immunoblot analysis of the effect of JIB-04 on Tat protein levels in 2D10 cells depleted of SHMT2 or BRCC36. (G) The effect of JIB-04 on wildtype or Tat delta K (+K41) STREP-tagged Tat proteins was shown by immunoblot. TAF4 served as loading control. (H) Model of the role of SHMT2 & BRCC36 in the release of Tat-K63Ub from destruction through chaperone-mediated autophagy or SQSMT1/p62-dependent macroautophagy. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.ppat.1007071), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")