| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Host | Sheep |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Immunogen | E. coli-derived recombinant human MERIT40 Asn182-Val329 Accession # Q9NWV8 |
| Specificity | Detects human MERIT40 in Western blots. |
| Source | N/A |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Sheep |
| Gene | BABAM1 |
| Purity Statement | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Publications |
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| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Reconstitution Instructions | Sterile PBS to a final concentration of 0.2 mg/mL. |
MERIT40 (MEdiator of Rap80 Interactions and Targeting subunit of 40 kDa; also NBA1 and c19orf62 and HSPC142) is a 40 kDa member of the MERIT40 family of proteins. It is ubiquitously expressed, and participates in the DNA damage response. At sites of double strand breaks, histones are phosphorylated, ubiquitinated, and deubiquitinated, with repairs performed by a sequentially formed complex that contains BRCA1, Rap80 and BRE. MERIT40 apparently serves as a scaffold protein that maintains the integrity of the complex. Human MERIT40 is 329 amino acids (aa) in length. It contains one VWF-A domain (aa 95-298) and six utilized Ser/Thr phosphorylation sites. There are three potential splice variants. One shows a 13 aa substitution for aa 234-329, a second possesses a 105 aa substitution for aa 263‑329, while a third shows a 37 aa substitution for aa 1-115, coupled to a deletion of aa 263-329. Over aa 182-329, human MERIT40 shares 95% aa identity with mouse MERIT40.
| Publication using AF6604 | Applications | Species |
|---|---|---|
| McMahon KA, Stroud DA, Gambin Y et al. Cavin3 released from caveolae interacts with BRCA1 to regulate the cellular stress response eLife 2021-06-18 [PMID: 34142659] |
Secondary Antibodies |
Isotype Controls |
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