![Western Blot: BRIP1/FANCJ Antibody [NBP1-31883] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with FANCJ antibody [N1N2], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/BRIP1-FANCJ-Antibody-Western-Blot-NBP1-31883-img0017.jpg "Western Blot: BRIP1/FANCJ Antibody [NBP1-31883] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with FANCJ antibody [N1N2], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Func, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human BRIP1/FANCJ. The exact sequence is proprietary. |
| Localization | Nucleus |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | BRIP1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Use in PLA reported in scientific literature (PMID:33445471). |
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| Theoretical MW | 141 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for BRIP1/FANCJ Antibody (NBP1-31883)Find related products by research area.
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| Gene Symbol | BRIP1 |
![Immunocytochemistry/Immunofluorescence: BRIP1/FANCJ Antibody [NBP1-31883] - 293T cells were fixed in 2% paraformaldehyde/culture medium at 37 degrees C for 30 min. Green: BRIP1 protein stained by FANCJ antibody [N1N2], N-term diluted at 1:500. Blue: Hoechst 33343 staining.](http://images.novusbio.com/fullsize/BRIP1-FANCJ-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31883-img0013.jpg "Immunocytochemistry/Immunofluorescence: BRIP1/FANCJ Antibody [NBP1-31883] - 293T cells were fixed in 2% paraformaldehyde/culture medium at 37 degrees C for 30 min. Green: BRIP1 protein stained by FANCJ antibody [N1N2], N-term diluted at 1:500. Blue: Hoechst 33343 staining.")
![Immunohistochemistry-Paraffin: BRIP1/FANCJ Antibody [NBP1-31883] - SAS xenograft. FANCJ antibody [N1N2], N-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/BRIP1-FANCJ-Antibody-Immunohistochemistry-Paraffin-NBP1-31883-img0014.jpg "Immunohistochemistry-Paraffin: BRIP1/FANCJ Antibody [NBP1-31883] - SAS xenograft. FANCJ antibody [N1N2], N-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Immunocytochemistry/Immunofluorescence: BRIP1/FANCJ Antibody [NBP1-31883] - Expression of BRIP1/FANCJ at the mRNA (graphs) and protein (photos) levels in iPSCs. Gene expression was assessed with RNA-seq in FACS-sorted bone marrow HSCs or with real-time RT-PCR in cultured iPSCs. The box-and-whiskers graphs show the median, 25th and 75th percentile (box) minimum, and maximum values (whiskers) of the FPKM (fragments per kilobase million) or relative expression in comparison to house-keeping genes (geometric mean for HPRT, B2M, and beta-actin), n = 4, * p < 0.05, ** p < 0.01, Mann–Whitney test (HSCs) or Wilcoxon test (iPSCs). Nuclei are labeled with DAPI. Scale bars: 20 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33445471/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp1-31883_rabbit-polyclonal-brip1-fancj-antibody-2552023153951.jpg "Immunocytochemistry/Immunofluorescence: BRIP1/FANCJ Antibody [NBP1-31883] - Expression of BRIP1/FANCJ at the mRNA (graphs) and protein (photos) levels in iPSCs. Gene expression was assessed with RNA-seq in FACS-sorted bone marrow HSCs or with real-time RT-PCR in cultured iPSCs. The box-and-whiskers graphs show the median, 25th and 75th percentile (box) minimum, and maximum values (whiskers) of the FPKM (fragments per kilobase million) or relative expression in comparison to house-keeping genes (geometric mean for HPRT, B2M, and beta-actin), n = 4, * p < 0.05, ** p < 0.01, Mann–Whitney test (HSCs) or Wilcoxon test (iPSCs). Nuclei are labeled with DAPI. Scale bars: 20 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33445471/) licensed under a CC-BY license.")
![Immunocytochemistry/ Immunofluorescence: BRIP1/FANCJ Antibody [NBP1-31883] - In situ proximity ligation assay (PLA) in HEK293T cells. (A) G4-BRIP1 & (B) G4-HMOX1 interactions (red dots) were visualized in the control group & after DNase or RNase digestion. Most of the G4 interactions were localized in the nucleus (arrows), but some were also present in the cytoplasm. Cells that were fixed in methanol & mouse 1H6 antibodies were used. Nuclei were counterstained with DAPI (blue), where the transmission bright-field images (BF) show the cell morphologies. Scale bar: 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33445471), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-31883_rabbit-polyclonal-brip1-fancj-antibody-310202415304253.jpg "Immunocytochemistry/ Immunofluorescence: BRIP1/FANCJ Antibody [NBP1-31883] - In situ proximity ligation assay (PLA) in HEK293T cells. (A) G4-BRIP1 & (B) G4-HMOX1 interactions (red dots) were visualized in the control group & after DNase or RNase digestion. Most of the G4 interactions were localized in the nucleus (arrows), but some were also present in the cytoplasm. Cells that were fixed in methanol & mouse 1H6 antibodies were used. Nuclei were counterstained with DAPI (blue), where the transmission bright-field images (BF) show the cell morphologies. Scale bar: 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33445471), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunocytochemistry/ Immunofluorescence: BRIP1/FANCJ Antibody [NBP1-31883] - Detection of the PLA signal in sorted hematopoietic stem cells. (A) G4–Brip1 & (B) G4–Hmox1 interactions (green dots) were visualized in HSCs & GMP & MPP cells. G4 interactions were visible in the nucleus (arrows) & in the cytoplasm. Nuclei were counterstained with DAPI (blue), where the transmission bright-field images (BF) show the cell morphologies. Cells were fixed in PFA & goat 1H6 antibodies were used. Scale bars: 5 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33445471), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-31883_rabbit-polyclonal-brip1-fancj-antibody-310202415175248.jpg "Immunocytochemistry/ Immunofluorescence: BRIP1/FANCJ Antibody [NBP1-31883] - Detection of the PLA signal in sorted hematopoietic stem cells. (A) G4–Brip1 & (B) G4–Hmox1 interactions (green dots) were visualized in HSCs & GMP & MPP cells. G4 interactions were visible in the nucleus (arrows) & in the cytoplasm. Nuclei were counterstained with DAPI (blue), where the transmission bright-field images (BF) show the cell morphologies. Cells were fixed in PFA & goat 1H6 antibodies were used. Scale bars: 5 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33445471), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: BRIP1/FANCJ Antibody [NBP1-31883] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRIP1/FANCJ antibody [N1N2], N-term (NBP1-31883) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31883_rabbit-polyclonal-brip1-fancj-antibody-91020242014665.jpg "Western Blot: BRIP1/FANCJ Antibody [NBP1-31883] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRIP1/FANCJ antibody [N1N2], N-term (NBP1-31883) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
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