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GABPA Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: GABPA Antibody [NBP1-32105] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GABPA protein stained by GABPA antibody [N2C2], Internal diluted at 1:1000. ...read more
Genetic Strategies: Western Blot: Rabbit Polyclonal GABPA Antibody [NBP1-32105] - Primary rat neonatal ventricular cardiomyocytes lysates with siRNA targeting Gabpa (siGabpa) and control (siCont). After 5 days of ...read more
Immunohistochemistry-Paraffin: GABPA Antibody [NBP1-32105] - Rat spleen. GABPA stained by GABPA antibody [N2C2], Internal diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunoprecipitation: GABPA Antibody [NBP1-32105] - Immunoprecipitation of GABPA protein from 293T whole cell extracts using 5 ug of GABPA antibody [N2C2], Internal. Western blot analysis was performed using GABPA ...read more
GABPA Antibody [NBP1-32105] - Mouse lymph node. GABPA stained by GABPA antibody [N2C2], Internal diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: GABPA Antibody [NBP1-32105] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GABPA antibody [N2C2], Internal (NBP1-32105) diluted at 1:1000. The ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IP, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

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GABPA Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human GABPA. The exact sequence is proprietary.
Localization
Nucleus
Predicted Species
Bovine (99%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GABPA
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:100-1:500
  • Knockdown Validated Validated for Knockdown from a verified customer review.
  • Western Blot 1:500-1:3000
Application Notes
IHC-P-Assay dependent
Theoretical MW
51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-32105 in the following applications:

Reactivity Notes

Xenopus laevis (80%), Chicken (88%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for GABPA Antibody

  • E4TF1-60
  • E4TF1ATranscription factor E4TF1-60
  • GA binding protein transcription factor, alpha subunit 60kDa
  • GA-binding protein alpha chain
  • GA-binding protein transcription factor, alpha subunit (60kD)
  • GABP subunit alpha
  • human nuclear respiratory factor-2 subunit alpha, 10
  • NFT2
  • NRF2
  • NRF2A
  • nuclear respiratory factor 2 alpha subunit
  • Nuclear respiratory factor 2 subunit alpha

Background

This gene encodes one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit. Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GABPA Antibody (NBP1-32105) (0)

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Review for GABPA Antibody (NBP1-32105) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NBP1-32105:
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WB
(1)
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Images Ratings Applications Species Date Details
  5
reviewed by:
Shinichi Oka
WB Rat 08/29/2023
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Summary

ApplicationWestern Blot
Sample Testedprimary neonatal ventricular cardiomyocytes
SpeciesRat
Lot42109

Comments

CommentsAfter 5 days of transfection with siRNA targeting rat Gabpa, the cells were lysed and subject to immunoblot analysis.

Experimental conditions of immunoblot:
Membrane: PDVF
Blocking: 5% BSA/TBS-T for 0.5 hour
First antibody (NBP1-32105) 1/5000 dilution (1 microL/5ml TBS-T) for overnight (16 hours) at 4 degrees.
Second antibody HRP-anti-Rabbit IgG (Cell Signaling Technology 7074) 1/5000 dilution (1 microL/5ml TBS-T) for 1 hour at room temperature.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GABPA Antibody (NBP1-32105) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Shinichi Oka
08/29/2023
Application: WB
Species: Rat

Bioinformatics

Gene Symbol GABPA