![Western Blot: PALB2 Antibody [NB100-60440] - Whole cell lysate (50 ug) from HeLa and cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PALB2 antibody NB100-60440 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/PALB2-Antibody-Western-Blot-NB100-60440-img0004.jpg "Western Blot: PALB2 Antibody [NB100-60440] - Whole cell lysate (50 ug) from HeLa and cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PALB2 antibody NB100-60440 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 675 and 725 of human partner and localizer of BRCA2 using the numbering given in entry NP_078951.2 (GeneID 79728). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PALB2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
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| Publication using NB100-60440 | Applications | Species |
|---|---|---|
| Martrat G, Maxwell CM, Tominaga E et al. Exploring the link between MORF4L1 and risk of breast cancer. Breast Cancer Res. 2011-04-05 [PMID: 21466675] (IP, Human) | IP | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for PALB2 Antibody (NB100-60440)Find related products by research area.
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The recent relationship of BRCA1 and 53BP1 The p53-binding protein 1 (53BP1) is a DNA damage response factor, which is recruited to nuclear structures at the site of DNA damage. DNA double-strand breaks (DSBs) are mutations that are detrimental to cell viability and genome stability, and m... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | PALB2 |
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| Uniprot |
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![Immunohistochemistry-Paraffin: PALB2 Antibody [NB100-60440] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- PALB2 used at a dilution of 1:1,000 (1 ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PALB2-Antibody-Immunohistochemistry-Paraffin-NB100-60440-img0003.jpg "Immunohistochemistry-Paraffin: PALB2 Antibody [NB100-60440] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- PALB2 used at a dilution of 1:1,000 (1 ug/ml). Detection: DAB")
![Immunoprecipitation: PALB2 Antibody [NB100-60440] - Detection of human PALB2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PALB2 antibody NB100-60440 used for IP at 6 ug per reaction. PALB2 was also immunoprecipitated by rabbit anti-PALB2 antibody NB100-60439. For blotting immunoprecipitated PALB2, NB100-60440 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/PALB2-Antibody-Immunoprecipitation-NB100-60440-img0005.jpg "Immunoprecipitation: PALB2 Antibody [NB100-60440] - Detection of human PALB2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PALB2 antibody NB100-60440 used for IP at 6 ug per reaction. PALB2 was also immunoprecipitated by rabbit anti-PALB2 antibody NB100-60439. For blotting immunoprecipitated PALB2, NB100-60440 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")