![Knockout Validated: BRE Antibody [NBP1-32304] - Wild-type (WT) and BRCC45 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BRCC45 antibody. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/BRE-Antibody-Western-Blot-NBP1-32304-img0008.jpg "Knockout Validated: BRE Antibody [NBP1-32304] - Wild-type (WT) and BRCC45 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BRCC45 antibody. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IP, KO |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human BRE. The exact sequence is proprietary. |
| Localization | Cytoplasm, Nucleus |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | BABAM2 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for BRE Antibody (NBP1-32304)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | BABAM2 |
![Immunocytochemistry/Immunofluorescence: BRE Antibody [NBP1-32304] - analysis of HeLa, using BRE antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/BRE-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32304-img0002.jpg "Immunocytochemistry/Immunofluorescence: BRE Antibody [NBP1-32304] - analysis of HeLa, using BRE antibody at 1:200 dilution.")
![Western Blot: BRE Antibody [NBP1-32304] - Sample: 1000 ug 293T whole cell lysate/extract A. Control with 2. 5 ug of preimmune rabbit IgG, B. Immunoprecipitation of BRE protein by 2. 5 ug of BRE antibody 10% SDS-PAGE gel.](http://images.novusbio.com/fullsize/BRE-Antibody-Western-Blot-NBP1-32304-img0004.jpg "Western Blot: BRE Antibody [NBP1-32304] - Sample: 1000 ug 293T whole cell lysate/extract A. Control with 2. 5 ug of preimmune rabbit IgG, B. Immunoprecipitation of BRE protein by 2. 5 ug of BRE antibody 10% SDS-PAGE gel.")
![Western Blot: BRE Antibody [NBP1-32304] - 30 ug C8D30 whole cell lysate/extract 10% SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/BRE-Antibody-Western-Blot-NBP1-32304-img0005.jpg "Western Blot: BRE Antibody [NBP1-32304] - 30 ug C8D30 whole cell lysate/extract 10% SDS-PAGE gel, antibody dilution 1:1000.")
![Western Blot: BRE Antibody [NBP1-32304] - A. 30 ug 293T whole cell lysate/extract, B. 30 ug A431 whole cell lysate/extract 10 % SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/BRE-Antibody-Western-Blot-NBP1-32304-img0006.jpg "Western Blot: BRE Antibody [NBP1-32304] - A. 30 ug 293T whole cell lysate/extract, B. 30 ug A431 whole cell lysate/extract 10 % SDS-PAGE gel, antibody dilution 1:1000.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")