RAP80 Antibody

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. TRAP80 is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. TRAP80 is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animals cells produce the protein, which stimulates the animals immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: ICC/IF, IHC-P, IM, Immunocytochemistry/ Immunofluorescence, WB, Western Blot.

Showing Publications 1 - 10 of 11. Show All 11 Publications. Collapse Publications.

Publications using NBP1-87156	Applications	Species
Jiang Q, Foglizzo M, Morozov YI et al. Autologous K63 deubiquitylation within the BRCA1-A complex licenses DNA damage recognition The Journal of cell biology 2022-09-05 [PMID: 35938958] (WB, Mouse, Human) Details: Dilution used 1:1000	WB	Mouse, Human
Sherker A, Chaudhary N, Adam S et al. Two redundant ubiquitin-dependent pathways of BRCA1 localization to DNA damage sites EMBO reports 2021-12-06 [PMID: 34726323] (Western Blot, Immunocytochemistry/ Immunofluorescence)	Western Blot, Immunocytochemistry/ Immunofluorescence
Nakamura K, Kustatscher G, Alabert C, et al. Proteome dynamics at broken replication forks reveal a distinct ATM-directed repair response suppressing DNA double-strand break ubiquitination Molecular cell 2021-01-11 [PMID: 33450211] (ICC/IF, IM, Human)	ICC/IF, IM	Human
Bodo S, Campagne C, Thin TH et al. Single-dose radiotherapy disables tumor cell homologous recombination via ischemia/reperfusion injury J. Clin. Invest. 2018-11-27 [PMID: 30480549] (IHC-P, Human)	IHC-P	Human
Yasuhara T, Kato R, Hagiwara Y et al. Human Rad52 Promotes XPG-Mediated R-loop Processing to Initiate Transcription-Associated Homologous Recombination Repair. Cell 2018-09-18 [PMID: 30245011] (Human)		Human
Watanabe S, Iimori M, Chan DV et al. MDC1 methylation mediated by lysine methyltransferases EHMT1 and EHMT2 regulates active ATM accumulation flanking DNA damage sites. Sci Rep. 2018-07-18 [PMID: 30022091] (ICC/IF, Human)	ICC/IF	Human
Baranes-Bachar K, Levy-Barda A, Oehler J et al. The Ubiquitin E3/E4 Ligase UBE4A Adjusts Protein Ubiquitylation and Accumulation at Sites of DNA Damage, Facilitating Double-Strand Break Repair. Mol. Cell. 2018-03-01 [PMID: 29499138] (Human)		Human
Ha K, Ma C, Lin H et al. The anaphase promoting complex impacts repair choice by protecting ubiquitin signalling at DNA damage sites. Nat Commun. 2017-06-12 [PMID: 28604711] (Human)		Human
Renaud E, Barascu A, Rosselli F. Impaired TIP60-mediated H4K16 acetylation accounts for the aberrant chromatin accumulation of 53BP1 and RAP80 in Fanconi anemia pathway-deficient cells. Nucleic Acids Res 2016-01-29 [PMID: 26446986] (WB)	WB
Kato K, Nakajima K, Ui A et al. Fine-Tuning of DNA Damage-Dependent Ubiquitination by OTUB2 Supports the DNA Repair Pathway Choice. Mol. Cell 2014-02-24 [PMID: 24560272] (ICC/IF, Human)	ICC/IF	Human
Lin ZP, Ratner ES, Whicker ME et al. Triapine Disrupts CtIP-Mediated Homologous Recombination Repair and Sensitizes Ovarian Cancer Cells to PARP and Topoisomerase Inhibitors. Mol. Cancer Res. 2014-02-28 [PMID: 24413181]
Show All 11 Publications. Collapse Publications.

Array NBP1-87156

• RAP80 Antibodies
• RAP80 Lysate
• RAP80 Proteins