JAB1 Antibody (2A10.8)

Jun activation domain-binding protein 1 (JAB1) also designated COP9 subunit 5 (COPS5) or SGN5 is a coactivator of AP1 transcription factor that also promotes degradation of the cyclin-dependent kinase inhibitor p27Kip1. JAB1 interacts with c-Jun AP1 containing complexes, and enhances transactivation from AP1 dependent promoters. It also interacts with Jun D but not with Jun B or v-Jun. JAB1 is highly conserved in evolution and is widely expressed in mammalian tissues. It is localized both the nucleus and the cytoplasm. It interacts with the cytoplasmic domain of the alphaL/beta2 integrin LFA1. Following LFA1 engagement the nuclear pool of JAB1 increases and activation of an AP1 driven promoter is enhanced. Interaction of JAB1 with the nuclear progesterone receptor and the steroid receptor activator (SRC1) was reported. JAB1 is a stability and activity regulator of Hypoxia inducible factor 1 (HIF1), a transcription factor that controls activation of several genes responsive to the cellular oxygen tension. The macrophage migration inhibitory factor (MIF) associates with JAB1 in the cytosol near the plasma membrane. Endogenous MIF inhibits JAB1 induced AP1 transcriptional activity. JAB1 is a subunit of the COP9 regulatory complex. COP9 cleaves the ubiquitin-like protein Nedd8 from the Cul1 subunit of SCF ubiquitin ligases. A metalloprotease motif in JAB1 plays a role in this isopeptidase activity. Breakdown of the cyclin dependent kinase inhibitor p27Kip1 is promoted by JAB1. The latter expression in several cancers inversely correlates with p27Kip1 and may reflect tumor aggressiveness. A possible involvement of JAB1 in atherosclerosis was also reported. Involvement of JAB1 in degradation of the suppressors p53 and smad4 was described recently.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC/IF, Immunoprecipitation, WB.

Showing Publications 1 - 10 of 11. Show All 11 Publications. Collapse Publications.

Publications using NB120-495	Applications	Species
Pines A, Schiffmacher DL, Lee SH et al. DDA1, a novel factor in transcription-coupled repair, modulates CRL4CSA dynamics at DNA damage-stalled RNA polymerase II Research square 2023-10-12 [PMID: 37886519] (Immunoprecipitation)	Immunoprecipitation
Israel S, Drexler Hca, Fuellen G, Boiani M The COP9 signalosome subunit 3 is necessary for early embryo survival by way of a stable protein deposit in mouse oocytes Molecular human reproduction 2021-07-15 [PMID: 34264319]
Karpiyevich M, Adjalley S, Mol M et al. Nedd8 hydrolysis by UCH proteases in Plasmodium parasites PLoS Pathog. 2019-10-01 [PMID: 31658303] (WB, Human)	WB	Human
Wang R, Wang H, Carrera I et al. COPS5 Overexpression Increases Amyloid Plaque Burden, Decreases Spinophilin-Immunoreactive-Puncta and Exacerbates Learning and Memory Deficits in the Mouse Brain J. Biol. Chem. 2015-02-20 [PMID: 25713139]
Wang H, Dey D, Carrera I et al. COPS5 (Jab1) increases ?-site processing of amyloid precursor protein and amyloid ? peptide generation by stabilizing RanBP9 protein levels. J Biol Chem 2013-08-07 [PMID: 23926111] (ICC/IF, WB, Mouse, Human)	ICC/IF, WB	Mouse, Human
Merk M, Zierow S, Leng L et al. The D-dopachrome tautomerase (DDT) gene product is a cytokine and functional homolog of macrophage migration inhibitory factor (MIF). Proc Natl Acad Sci USA 2011-08-01 [PMID: 21817065] (WB, ICC/IF, Human)	WB, ICC/IF	Human
McCall CM, Miliani de Marval PL, Chastain PD 2nd et al. Human immunodeficiency virus type 1 Vpr-binding protein VprBP, a WD40 protein associated with the DDB1-CUL4 E3 ubiquitin ligase, is essential for DNA replication and embryonic development. Mol Cell Biol 2008-09-01 [PMID: 18606781] (ICC/IF, WB, Human)	ICC/IF, WB	Human
Yun J, Tomida A, Andoh T et al. Interaction between glucose-regulated destruction domain of DNA topoisomerase IIalpha and MPN domain of Jab1/CSN5. J Biol Chem 2004-07-01 [PMID: 15126503] (WB, ICC/IF, Human)	WB, ICC/IF	Human
Doronkin S, Djagaeva I, Beckendorf SK. CSN5/Jab1 mutations affect axis formation in the Drosophila oocyte by activating a meiotic checkpoint. Development 2002-11-01 [PMID: 12397113] (ICC/IF, WB, Human)	ICC/IF, WB	Human
Korbonits M, Chahal HS, Kaltsas G et al. Expression of phosphorylated p27(kip1) protein and Jun activation domain-binding protein 1 in human pituitary tumors. J Clin Endocrinol Metab 2002-06-01 [PMID: 12050228] (WB, ICC/IF, Human)	WB, ICC/IF	Human
Gemmill RM, Bemis LT, Lee JP et al. The TRC8 hereditary kidney cancer gene suppresses growth and functions with VHL in a common pathway. Oncogene 2002-05-01 [PMID: 12032852] (ICC/IF, WB, Human)	ICC/IF, WB	Human
Show All 11 Publications. Collapse Publications.

Array NB120-495

• JAB1 Antibodies
• JAB1 Lysate
• JAB1 Proteins