Western Blot: BMP-2 Antibody - BSA Free [NBP1-19751] - Western blots and summary graphs of BMP2 and alpha-tubulin expression in abdominal (A; black) and gluteal (G; white) AT (A; n = 5 women; 37-44 years, BMI 24.1-27.1 ...read more
Immunohistochemistry: BMP-2 Antibody - BSA Free [NBP1-19751] - Vascular cells reprogrammed from young vs. old donors show gene expression and functional differences. Representative images of BMP2 from skin biopsies ...read more
Western Blot: BMP-2 Antibody - BSA Free [NBP1-19751] - Extracts from HUVEC cells.
Western Blot: BMP-2 Antibody - BSA Free [NBP1-19751] - Protein expression analysis. Immunostaining for BMP-2, myostatin, and CD44 was evaluated by counting the number of positive fibers/cells on 25-high power field ...read more
Immunohistochemistry: BMP-2 Antibody - BSA Free [NBP1-19751] - Analysis of BMP2 in mouse heart using DAB with hematoxylin counterstain.
Immunohistochemistry-Paraffin: BMP-2 Antibody - BSA Free [NBP1-19751] - Molecular analysis of BMP-2 expression. In order to verify the correlation between BMP-2 and satellite cells activity we performed both western ...read more
Immunohistochemistry: BMP-2 Antibody - BSA Free [NBP1-19751] - Molecular analysis of BMP-2 expression. In order to verify the correlation between BMP-2 & satellite cells activity we performed both western blot & ...read more
Immunohistochemistry-Paraffin: BMP-2 Antibody - BSA Free [NBP1-19751] - BMP-2 & satellite stem cells in muscle regenerations. ((a)-(b)) Hematoxylin & eosin sections of muscle biopsies showed a significant increase of ...read more
Immunohistochemistry: BMP-2 Antibody - BSA Free [NBP1-19751] - BMP-2 & satellite stem cells in muscle regenerations. ((a)-(b)) Hematoxylin & eosin sections of muscle biopsies showed a significant increase of fat tissue ...read more
Immunocytochemistry/ Immunofluorescence: BMP-2 Antibody - BSA Free [NBP1-19751] - BMD macrophages & splenic macrophages express nBMP2, which is decreased in the nuclei of nBmp2NLStm mutant macrophages. (a) BMD ...read more
Immunocytochemistry/ Immunofluorescence: BMP-2 Antibody - BSA Free [NBP1-19751] - BMD macrophages & splenic macrophages express nBMP2, which is decreased in the nuclei of nBmp2NLStm mutant macrophages. (a) BMD ...read more
Immunohistochemistry-Paraffin: BMP-2 Antibody - BSA Free [NBP1-19751] - BMP-2 & satellite stem cells in muscle regenerations. ((a)-(b)) Hematoxylin & eosin sections of muscle biopsies showed a significant increase of ...read more
Western Blot: BMP-2 Antibody - BSA Free [NBP1-19751] - Protein expression analysis. Immunostaining for BMP-2, myostatin, & CD44 was evaluated by counting the number of positive fibers/cells on 25-high power field (HPF), ...read more
Western Blot: BMP-2 Antibody - BSA Free [NBP1-19751] - BMP2 is a paracrine factor in WAT & regulates adipogenic differentiation in a depot-specific manner. a, b, c Western blots & summary graphs of BMP2 & alpha ...read more
Immunocytochemistry/ Immunofluorescence reported in scientific literature (PMID 30700748)
Immunohistochemistry 1:50-1:200
Immunohistochemistry-Paraffin 1:50-1:200
Western Blot 0.5 ug/ml
Application Notes
In Western Blot, a band is seen ~44 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Theoretical MW
44 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Use in Canine reported in scientific literature (PMID:34311726)
Packaging, Storage & Formulations
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified
Alternate Names for BMP-2 Antibody - BSA Free
BDA2
BMP2
BMP-2
BMP-2A
BMP2ABone morphogenetic protein 2A
bone morphogenetic protein 2
SSFSC
Background
Bone morphogenic protein 2 is required for effective healing of fractures in bone. It is necessary for the expression of transcription factors that regulate osteogenesis and differentiation of progenitor cells into mature osteoblasts. Embryologically, BMP2 is important in mesenchymal maturation to fetal valves. The same mechanism of maturation is also utilized by tumor molecular machinery once mesenchymal stem cells are recruited to tumor microenvironments, leading to many recent attempts to generate BMP inhibitors in the treatment of ovarian and colon cancer. The activity of BMP2 is antagonized by the activity of Bambi and Crim1. BMP2 is an interesting target for distinguishing the mechanism by which normal and necessary maturation processes are mutated in tumorigenesis.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Harper CM The Role of Type VIII Collagen in Atherosclerotic Plaque Stability Thesis 2020-01-01
Ko B, Ryuk J, Hwang J Allium fistulosum (Welsh onion) and Portulaca oleracea increase longitudinal bone growth in weanling rats possibly by promoting TGF-beta and IGF-1 signaling Journal of Functional Foods 2019-07-01 (IB, Rat, Human)
FAQs for BMP-2 Antibody (NBP1-19751). (Showing 1 - 2 of 2 FAQs).
I am interested in Bone Morphogenic Protein 2 (BMP-2) for clinical trial in humans. I am using this protein in stem cells culture for future use in humans. Do you have BMP-2 in pharma grade that I can use for humans?
Our antibodies are for research purposes only unfortunately. I am sorry we cannot be of further help.
We onced ordered an anti BMP2 antibody (NBP1-19751) from your company, and have gotten great WB results. Your product has been included in our manuscript. Now reviewers are asking about the cross activity of this antibody with other BMPs, such as BMP6 or BMP9. Did you ever check the cross-react of the BMP2 antibody?
Unfortunately, we have not tested this antibody against other/recombinant BMPs so far but I can assure you that it will not be expected to react with any other BMPs. NBP1-19751 was generated against an immunogen peptide from AA250-350 of BMP2 protein [Swiss-Prot# P12643], and this sequence does not show any sequence similarity/homology with any other BMPs.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Western Blot: BMP-2 Antibody - BSA Free [NBP1-19751] - Western blots and summary graphs of BMP2 and alpha-tubulin expression in abdominal (A; black) and gluteal (G; white) AT (A; n = 5 women; 37-44 years, BMI 24.1-27.1 kg/m2), and BMP2 and beta-actin expression in proliferating (b) and differentiated (c) immortalised preadipocytes (n = 3). Relative protein expression data presented as mean +/- SEM with individual data points overlaid (grey dots); data analysed by paired t-test. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31324879/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BMP-2 Antibody - BSA Free-Western Blot-NBP1-19751-img0013.jpg "Western Blot: BMP-2 Antibody - BSA Free [NBP1-19751] - Western blots and summary graphs of BMP2 and alpha-tubulin expression in abdominal (A; black) and gluteal (G; white) AT (A; n = 5 women; 37-44 years, BMI 24.1-27.1 kg/m2), and BMP2 and beta-actin expression in proliferating (b) and differentiated (c) immortalised preadipocytes (n = 3). Relative protein expression data presented as mean +/- SEM with individual data points overlaid (grey dots); data analysed by paired t-test. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31324879/) licensed under a CC-BY license.")
![Immunocytochemistry Osteopontin/OPN Antibody [Unconjugated]](https://images.novusbio.com/images2/Osteopontin_AF808_Immunocytochemistry__Immunofluorescence_23325.jpg)
![Neutralization Osteopontin/OPN Antibody [Unconjugated]](https://images.novusbio.com/images2/Osteopontin_AF808_Block_Neutralize_8975.jpg)
![Immunohistochemistry Osteopontin/OPN Antibody [Unconjugated]](https://images.novusbio.com/images2/Osteopontin_AF808_Immunohistochemistry_9333.jpg)
![Immunohistochemistry: BMP-2 Antibody - BSA Free [NBP1-19751] - Vascular cells reprogrammed from young vs. old donors show gene expression and functional differences. Representative images of BMP2 from skin biopsies obtained from young vs. old donors. Scale bars: 50 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32896271/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BMP-2-Antibody---BSA-Free-Immunohistochemistry-NBP1-19751-img0012.jpg "Immunohistochemistry: BMP-2 Antibody - BSA Free [NBP1-19751] - Vascular cells reprogrammed from young vs. old donors show gene expression and functional differences. Representative images of BMP2 from skin biopsies obtained from young vs. old donors. Scale bars: 50 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32896271/) licensed under a CC-BY license.")
![Western Blot: BMP-2 Antibody - BSA Free [NBP1-19751] - Extracts from HUVEC cells.](http://images.novusbio.com/fullsize/BMP-2-Antibody---BSA-Free-Western-Blot-NBP1-19751-img0008.jpg "Western Blot: BMP-2 Antibody - BSA Free [NBP1-19751] - Extracts from HUVEC cells.")
![Western Blot: BMP-2 Antibody - BSA Free [NBP1-19751] - Protein expression analysis. Immunostaining for BMP-2, myostatin, and CD44 was evaluated by counting the number of positive fibers/cells on 25-high power field (HPF), whereas western blot for BMP-2 was evaluated by lines densitometry. Western blot lines show a higher expression of BMP-2 in OA patients compared to OP group. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/sci/2015/469459/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BMP-2-Antibody---BSA-Free-Western-Blot-NBP1-19751-img0010.jpg "Western Blot: BMP-2 Antibody - BSA Free [NBP1-19751] - Protein expression analysis. Immunostaining for BMP-2, myostatin, and CD44 was evaluated by counting the number of positive fibers/cells on 25-high power field (HPF), whereas western blot for BMP-2 was evaluated by lines densitometry. Western blot lines show a higher expression of BMP-2 in OA patients compared to OP group. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/sci/2015/469459/), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: BMP-2 Antibody - BSA Free [NBP1-19751] - Paraffin-embedded human heart tissue.](http://images.novusbio.com/fullsize/BMP-2-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP1-19751-img0007.jpg "Immunohistochemistry-Paraffin: BMP-2 Antibody - BSA Free [NBP1-19751] - Paraffin-embedded human heart tissue.")
![Immunohistochemistry: BMP-2 Antibody - BSA Free [NBP1-19751] - Analysis of BMP2 in mouse heart using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/BMP-2-Antibody---BSA-Free-Immunohistochemistry-NBP1-19751-img0009.jpg "Immunohistochemistry: BMP-2 Antibody - BSA Free [NBP1-19751] - Analysis of BMP2 in mouse heart using DAB with hematoxylin counterstain.")
![Immunohistochemistry-Paraffin: BMP-2 Antibody - BSA Free [NBP1-19751] - Molecular analysis of BMP-2 expression. In order to verify the correlation between BMP-2 and satellite cells activity we performed both western blot and immune-gold analysis. In muscle biopsies of OA patients we found several large satellite cells syncytium (arrows). High magnifications show immunolabeling for BMP-2 in perinuclear areas (squares) (40.000x). Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/sci/2015/469459/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BMP-2-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP1-19751-img0011.jpg "Immunohistochemistry-Paraffin: BMP-2 Antibody - BSA Free [NBP1-19751] - Molecular analysis of BMP-2 expression. In order to verify the correlation between BMP-2 and satellite cells activity we performed both western blot and immune-gold analysis. In muscle biopsies of OA patients we found several large satellite cells syncytium (arrows). High magnifications show immunolabeling for BMP-2 in perinuclear areas (squares) (40.000x). Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/sci/2015/469459/), licensed under a CC-BY license.")
![Immunohistochemistry: BMP-2 Antibody - BSA Free [NBP1-19751] - Molecular analysis of BMP-2 expression. In order to verify the correlation between BMP-2 & satellite cells activity we performed both western blot & immune-gold analysis. In muscle biopsies of OA patients we found several large satellite cells syncytium (arrows) (a). High magnifications show immunolabeling for BMP-2 in perinuclear areas (squares) (40.000x) (b). BMP-2 molecules were expressed in the satellite cells syncytium cytoplasm (squares) & next to mitochondria (insert) (40.000 & 60.000x) (c). Numerous BMP-2 molecules were found in the fiber body (40.000x) (d). OP patients do not express BMP-2 in satellite cells (10.000x & 40.000x) ((e)-(f)). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26101529), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-19751_rabbit-polyclonal-bmp-2-antibody-310202415293327.jpg "Immunohistochemistry: BMP-2 Antibody - BSA Free [NBP1-19751] - Molecular analysis of BMP-2 expression. In order to verify the correlation between BMP-2 & satellite cells activity we performed both western blot & immune-gold analysis. In muscle biopsies of OA patients we found several large satellite cells syncytium (arrows) (a). High magnifications show immunolabeling for BMP-2 in perinuclear areas (squares) (40.000x) (b). BMP-2 molecules were expressed in the satellite cells syncytium cytoplasm (squares) & next to mitochondria (insert) (40.000 & 60.000x) (c). Numerous BMP-2 molecules were found in the fiber body (40.000x) (d). OP patients do not express BMP-2 in satellite cells (10.000x & 40.000x) ((e)-(f)). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26101529), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunohistochemistry-Paraffin: BMP-2 Antibody - BSA Free [NBP1-19751] - BMP-2 & satellite stem cells in muscle regenerations. ((a)-(b)) Hematoxylin & eosin sections of muscle biopsies showed a significant increase of fat tissue in OA (a) as compared to OP patients (b) (40x). (c) Image showed numerous BMP-2 positive fibers (40x). Often, in OP patients we did not observed BMP-2 expression (40x) (d). The Immunohistochemistry for myostatin was negative in OA muscle tissue (40x) (e). Muscle biopsies of OP group showed high/moderate expression of myostatin (40x) (f) inversely related to BMP-2 immunostain. Groups of satellite cells CD44 positive were focally dispersed in the tissue (200x) of OA patients (g) higher than that observed in OP patients (200x) (h). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26101529), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-19751_rabbit-polyclonal-bmp-2-antibody-310202415334910.jpg "Immunohistochemistry-Paraffin: BMP-2 Antibody - BSA Free [NBP1-19751] - BMP-2 & satellite stem cells in muscle regenerations. ((a)-(b)) Hematoxylin & eosin sections of muscle biopsies showed a significant increase of fat tissue in OA (a) as compared to OP patients (b) (40x). (c) Image showed numerous BMP-2 positive fibers (40x). Often, in OP patients we did not observed BMP-2 expression (40x) (d). The Immunohistochemistry for myostatin was negative in OA muscle tissue (40x) (e). Muscle biopsies of OP group showed high/moderate expression of myostatin (40x) (f) inversely related to BMP-2 immunostain. Groups of satellite cells CD44 positive were focally dispersed in the tissue (200x) of OA patients (g) higher than that observed in OP patients (200x) (h). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26101529), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunohistochemistry: BMP-2 Antibody - BSA Free [NBP1-19751] - BMP-2 & satellite stem cells in muscle regenerations. ((a)-(b)) Hematoxylin & eosin sections of muscle biopsies showed a significant increase of fat tissue in OA (a) as compared to OP patients (b) (40x). (c) Image showed numerous BMP-2 positive fibers (40x). Often, in OP patients we did not observed BMP-2 expression (40x) (d). The Immunohistochemistry for myostatin was negative in OA muscle tissue (40x) (e). Muscle biopsies of OP group showed high/moderate expression of myostatin (40x) (f) inversely related to BMP-2 immunostain. Groups of satellite cells CD44 positive were focally dispersed in the tissue (200x) of OA patients (g) higher than that observed in OP patients (200x) (h). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26101529), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-19751_rabbit-polyclonal-bmp-2-antibody-31020241534532.jpg "Immunohistochemistry: BMP-2 Antibody - BSA Free [NBP1-19751] - BMP-2 & satellite stem cells in muscle regenerations. ((a)-(b)) Hematoxylin & eosin sections of muscle biopsies showed a significant increase of fat tissue in OA (a) as compared to OP patients (b) (40x). (c) Image showed numerous BMP-2 positive fibers (40x). Often, in OP patients we did not observed BMP-2 expression (40x) (d). The Immunohistochemistry for myostatin was negative in OA muscle tissue (40x) (e). Muscle biopsies of OP group showed high/moderate expression of myostatin (40x) (f) inversely related to BMP-2 immunostain. Groups of satellite cells CD44 positive were focally dispersed in the tissue (200x) of OA patients (g) higher than that observed in OP patients (200x) (h). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26101529), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunocytochemistry/ Immunofluorescence: BMP-2 Antibody - BSA Free [NBP1-19751] - BMD macrophages & splenic macrophages express nBMP2, which is decreased in the nuclei of nBmp2NLStm mutant macrophages. (a) BMD macrophages & (b) splenic macrophages were stained with anti-BMP2 antibody (green) & counterstained with DAPI (blue), demonstrating that nBMP2 is expressed & localized to the nucleus in wild type macrophages, & that nuclear translocation of nBMP2 is inhibited in mutant macrophages. BMP2 labeling within the cytoplasm is present in both wild type & mutant cells as expected, because the targeted mutation allows translation of nBMP2 in the cytoplasm but inhibits nuclear translocation, & it allows normal synthesis & secretion of conventional BMP2. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30700748), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-19751_rabbit-polyclonal-bmp-2-antibody-310202415382442.jpg "Immunocytochemistry/ Immunofluorescence: BMP-2 Antibody - BSA Free [NBP1-19751] - BMD macrophages & splenic macrophages express nBMP2, which is decreased in the nuclei of nBmp2NLStm mutant macrophages. (a) BMD macrophages & (b) splenic macrophages were stained with anti-BMP2 antibody (green) & counterstained with DAPI (blue), demonstrating that nBMP2 is expressed & localized to the nucleus in wild type macrophages, & that nuclear translocation of nBMP2 is inhibited in mutant macrophages. BMP2 labeling within the cytoplasm is present in both wild type & mutant cells as expected, because the targeted mutation allows translation of nBMP2 in the cytoplasm but inhibits nuclear translocation, & it allows normal synthesis & secretion of conventional BMP2. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30700748), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunocytochemistry/ Immunofluorescence: BMP-2 Antibody - BSA Free [NBP1-19751] - BMD macrophages & splenic macrophages express nBMP2, which is decreased in the nuclei of nBmp2NLStm mutant macrophages. (a) BMD macrophages & (b) splenic macrophages were stained with anti-BMP2 antibody (green) & counterstained with DAPI (blue), demonstrating that nBMP2 is expressed & localized to the nucleus in wild type macrophages, & that nuclear translocation of nBMP2 is inhibited in mutant macrophages. BMP2 labeling within the cytoplasm is present in both wild type & mutant cells as expected, because the targeted mutation allows translation of nBMP2 in the cytoplasm but inhibits nuclear translocation, & it allows normal synthesis & secretion of conventional BMP2. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30700748), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-19751_rabbit-polyclonal-bmp-2-antibody-3102024153568.jpg "Immunocytochemistry/ Immunofluorescence: BMP-2 Antibody - BSA Free [NBP1-19751] - BMD macrophages & splenic macrophages express nBMP2, which is decreased in the nuclei of nBmp2NLStm mutant macrophages. (a) BMD macrophages & (b) splenic macrophages were stained with anti-BMP2 antibody (green) & counterstained with DAPI (blue), demonstrating that nBMP2 is expressed & localized to the nucleus in wild type macrophages, & that nuclear translocation of nBMP2 is inhibited in mutant macrophages. BMP2 labeling within the cytoplasm is present in both wild type & mutant cells as expected, because the targeted mutation allows translation of nBMP2 in the cytoplasm but inhibits nuclear translocation, & it allows normal synthesis & secretion of conventional BMP2. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30700748), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunohistochemistry-Paraffin: BMP-2 Antibody - BSA Free [NBP1-19751] - BMP-2 & satellite stem cells in muscle regenerations. ((a)-(b)) Hematoxylin & eosin sections of muscle biopsies showed a significant increase of fat tissue in OA (a) as compared to OP patients (b) (40x). (c) Image showed numerous BMP-2 positive fibers (40x). Often, in OP patients we did not observed BMP-2 expression (40x) (d). The Immunohistochemistry for myostatin was negative in OA muscle tissue (40x) (e). Muscle biopsies of OP group showed high/moderate expression of myostatin (40x) (f) inversely related to BMP-2 immunostain. Groups of satellite cells CD44 positive were focally dispersed in the tissue (200x) of OA patients (g) higher than that observed in OP patients (200x) (h). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26101529), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-19751_rabbit-polyclonal-bmp-2-antibody-310202415371974.jpg "Immunohistochemistry-Paraffin: BMP-2 Antibody - BSA Free [NBP1-19751] - BMP-2 & satellite stem cells in muscle regenerations. ((a)-(b)) Hematoxylin & eosin sections of muscle biopsies showed a significant increase of fat tissue in OA (a) as compared to OP patients (b) (40x). (c) Image showed numerous BMP-2 positive fibers (40x). Often, in OP patients we did not observed BMP-2 expression (40x) (d). The Immunohistochemistry for myostatin was negative in OA muscle tissue (40x) (e). Muscle biopsies of OP group showed high/moderate expression of myostatin (40x) (f) inversely related to BMP-2 immunostain. Groups of satellite cells CD44 positive were focally dispersed in the tissue (200x) of OA patients (g) higher than that observed in OP patients (200x) (h). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26101529), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: BMP-2 Antibody - BSA Free [NBP1-19751] - Protein expression analysis. Immunostaining for BMP-2, myostatin, & CD44 was evaluated by counting the number of positive fibers/cells on 25-high power field (HPF), whereas western blot for BMP-2 was evaluated by lines densitometry. (a) Notably, we found that OA muscle biopsies showed a significantly higher number of BMP-2-positive fibers (293.0 ± 35.4) as compared with muscle of OP patients (162.1 ± 33.7) (p < 0.0001). (b) Western blot lines show a higher expression of BMP-2 in OA patients compared to OP group. (c) The number of myostatin positive fibers in OP patients was significantly higher compared to OA group (p < 0.0200). (d) CD44 expression shows a significantly different rate of CD44 positive cells in OA group as compared with OP (p < 0.0020). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26101529), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-19751_rabbit-polyclonal-bmp-2-antibody-310202416182640.jpg "Western Blot: BMP-2 Antibody - BSA Free [NBP1-19751] - Protein expression analysis. Immunostaining for BMP-2, myostatin, & CD44 was evaluated by counting the number of positive fibers/cells on 25-high power field (HPF), whereas western blot for BMP-2 was evaluated by lines densitometry. (a) Notably, we found that OA muscle biopsies showed a significantly higher number of BMP-2-positive fibers (293.0 ± 35.4) as compared with muscle of OP patients (162.1 ± 33.7) (p < 0.0001). (b) Western blot lines show a higher expression of BMP-2 in OA patients compared to OP group. (c) The number of myostatin positive fibers in OP patients was significantly higher compared to OA group (p < 0.0200). (d) CD44 expression shows a significantly different rate of CD44 positive cells in OA group as compared with OP (p < 0.0020). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26101529), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: BMP-2 Antibody - BSA Free [NBP1-19751] - BMP2 is a paracrine factor in WAT & regulates adipogenic differentiation in a depot-specific manner. a, b, c Western blots & summary graphs of BMP2 & alpha -tubulin expression in abdominal (A; black) & gluteal (G; white) AT (A; n = 5 women; 37–44 years, BMI 24.1–27.1 kg/m2), & BMP2 & beta -actin expression in proliferating (b) & differentiated (c) immortalised preadipocytes (n = 3). Relative protein expression data presented as mean ± SEM with individual data points overlaid (grey dots); data analysed by paired t-test. dBMP2 mRNA expression in primary abdominal (solid line) & gluteal (dashed line) preadipocytes during 3 days of proliferation (P) followed by 14 days of differentiation (d) (n = 8; 4 men, 4 women; age 32–44 years, BMI 20.5–26 kg/m2). mRNA expression normalised to PPIA. Data analysed by repeated measures ANOVA. e, f, g, h Proliferation analysis (e) in immortalised preadipocytes after 48 h culture in growth medium supplemented with BMP2 (n = 4). Triacylglycerol (TAG) accumulation (f) & PPARG2 (g) & ADIPOQ (h) mRNA expression in immortalised abdominal (black) & gluteal (white) preadipocytes after 14 day culture in BMP2-supplemented adipogenic medium (n = 3). mRNA expression normalised to 18 s. All data presented as mean fold change relative to vehicle ± SEM with individual data points overlaid (grey dots). Data analysed by ANOVA with Bonferroni post-hoc test (e, f, g, h); *p < 0.05, **p < 0.01, ***p < 0.001, compared to same depot vehicle; ‡p < 0.001, compared to same depot 5 ng/ml treatment Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31324879), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-19751_rabbit-polyclonal-bmp-2-antibody-310202416163764.jpg "Western Blot: BMP-2 Antibody - BSA Free [NBP1-19751] - BMP2 is a paracrine factor in WAT & regulates adipogenic differentiation in a depot-specific manner. a, b, c Western blots & summary graphs of BMP2 & alpha -tubulin expression in abdominal (A; black) & gluteal (G; white) AT (A; n = 5 women; 37–44 years, BMI 24.1–27.1 kg/m2), & BMP2 & beta -actin expression in proliferating (b) & differentiated (c) immortalised preadipocytes (n = 3). Relative protein expression data presented as mean ± SEM with individual data points overlaid (grey dots); data analysed by paired t-test. dBMP2 mRNA expression in primary abdominal (solid line) & gluteal (dashed line) preadipocytes during 3 days of proliferation (P) followed by 14 days of differentiation (d) (n = 8; 4 men, 4 women; age 32–44 years, BMI 20.5–26 kg/m2). mRNA expression normalised to PPIA. Data analysed by repeated measures ANOVA. e, f, g, h Proliferation analysis (e) in immortalised preadipocytes after 48 h culture in growth medium supplemented with BMP2 (n = 4). Triacylglycerol (TAG) accumulation (f) & PPARG2 (g) & ADIPOQ (h) mRNA expression in immortalised abdominal (black) & gluteal (white) preadipocytes after 14 day culture in BMP2-supplemented adipogenic medium (n = 3). mRNA expression normalised to 18 s. All data presented as mean fold change relative to vehicle ± SEM with individual data points overlaid (grey dots). Data analysed by ANOVA with Bonferroni post-hoc test (e, f, g, h); *p < 0.05, **p < 0.01, ***p < 0.001, compared to same depot vehicle; ‡p < 0.001, compared to same depot 5 ng/ml treatment Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31324879), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Bioactivity BMP-2 [Unconjugated]](https://images.novusbio.com/images/protein/355-bm_recombinant-human-mouse-rat-bmp-2-protein-bioactivity-181120209742.jpg)
![SDS-PAGE BMP-4 [Unconjugated]](https://images.novusbio.com/images2/BMP-4_314-BP_100.jpg)
![Bioactivity BMP-4 [Unconjugated]](https://images.novusbio.com/images2/314-bp_recombinant-human-bmp-4-protein-1882021123744.jpg)
![Bioactivity BMP-7 [Unconjugated]](https://images.novusbio.com/images/protein/354-bp_recombinant-human-bmp-7-protein-bioactivity-7122020141940.jpg)
![Cell Culture BMP-7 [Unconjugated]](https://images.novusbio.com/images/354-bp_recombinant-human-bmp-7-protein-cell-culture-252023113257..jpg)
![Immunocytochemistry RUNX2/CBFA1 Antibody (232902) [Unconjugated]](https://images.novusbio.com/images2/RUNX2_MAB2006_Immunocytochemistry_13309.jpg)
![Immunohistochemistry RUNX2/CBFA1 Antibody (232902) [Unconjugated]](https://images.novusbio.com/images/mab2006_human-runx2-cbfa1-mab-clone-232902-812025555286.jpg)
![N/A SLPI [HRP]](https://images.novusbio.com/images2/DATA_SLPI_DPI00_ELISA_792.jpg)
![N/A SLPI [HRP]](https://images.novusbio.com/images2/SLPI_DPI00_ELISA_160.jpg)
![N/A CCL27/CTACK [HRP]](https://images.novusbio.com/images2/CCL27_DCC270_ELISA_54.jpg)
![N/A CCL27/CTACK [HRP]](https://images.novusbio.com/images2/DATA_CCL27_DCC270_ELISA_615.jpg)
![Immunohistochemistry Smad1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Smad1_AF2039_Immunohistochemistry_10106.jpg)
![Western Blot Smad1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Smad1_AF2039_Western_Blot_5162.jpg)
![Simple Western Smad1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Smad1_AF2039_Simple_Western_16677.jpg)
![SDS-PAGE BMP-6 [Unconjugated]](https://images.novusbio.com/images2/BMP-6_507-BP_374.jpg)
![Bioactivity BMP-6 [Unconjugated]](https://images.novusbio.com/images2/BMP-6_507-BP_376.jpg)
![Bioactivity Noggin [Unconjugated]](https://images.novusbio.com/images2/Noggin_6057NG_2865.jpg)
![Bioactivity Noggin [Unconjugated]](https://images.novusbio.com/images2/Noggin_6057NG_923.jpg)
![Bioactivity Noggin [Unconjugated]](https://images.novusbio.com/images2/6057-ng_recombinant-human-noggin-protein-bioactivity-1022023121039.jpg)
![FGF basic/FGF2/bFGF [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "FGF basic/FGF2/bFGF [Unconjugated]")
![N/A VEGF [HRP]](https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_871.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_872.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/VEGF_DVE00_ELISA_208.jpg)
![Bioactivity Sonic Hedgehog/Shh [Unconjugated]](https://images.novusbio.com/images2/Sonic_Hedgehog_464SH_2840.jpg)
![SDS-PAGE Sonic Hedgehog/Shh [Unconjugated]](https://images.novusbio.com/images2/Sonic_Hedgehog_464-SH_341.jpg)
![SEC-MALS Sonic Hedgehog/Shh [Unconjugated]](https://images.novusbio.com/images/464-sh_recombinant-mouse-sonic-hedgehog-shh-c25ii-n-terminus-sec-mals-642023224324.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
