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beta-III Tubulin Antibody (TuJ-1) [NL557] - Neuron-Specific

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beta ‑III Tubulin was detected in immersion fixed 7 day differentiated rat cortical stem cells using a 1:10 dilution of Neuron-specific beta ‑III Tubulin (Clone TuJ‑1) NorthernLights™-557 Conjugated Monoclonal Antibody (Catalog # NL1195R) for 3 hours at room temperature. Cells were stained (red) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
beta ‑III Tubulin was detected in immersion fixed 7 day differentiated rat cortical stem cells using a 1:10 dilution of Neuron-specific beta ‑III Tubulin (Clone TuJ‑1) NorthernLights™-557 Conjugated ...read more
Assessment of the roles of macrophages in corneal development. a Co-staining of limbal blood vessels/corneal nerve fibers and macrophages in mice at postnatal day 7 (P7). The upper image shows the immunostaining of the ...read more

Product Details

Summary
Reactivity All-MultiSpecies Glossary
Applications ICC/IF
Clone
TuJ-1
Clonality
Monoclonal
Host
Mouse
Conjugate
NL557

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Allophycocyanin
IC1195A
Biotin
BAM1195
NL493
NL1195G
NL637
NL1195V
PerCP (Peridinin-chlorophyll Protein Complex)
IC1195C
Unconjugated
MAB1195
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB1195
Carrier Free
IC1195C
Carrier Free
MAB1195
Carrier Free
IC1195A
Carrier Free
NL1195G
Carrier Free
NL1195V
Carrier Free
MAB1195-SP
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beta-III Tubulin Antibody (TuJ-1) [NL557] - Neuron-Specific Summary

Immunogen
Rat brain-derived microtubules
Specificity
Detects mammalian and chicken neuron-specific beta -III tubulin but not other beta -tubulin isotypes in Western blots.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
TUBB3
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 1:10 dilution
Publications
Read Publications using
NL1195R in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Buffer
Supplied as a 10X solution of antibody in PBS containing sodium azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for beta-III Tubulin Antibody (TuJ-1) [NL557] - Neuron-Specific

  • Beta3-tubulin
  • beta-4
  • betaIII Tubulin
  • beta-III Tubulin
  • beta-Tubulin III
  • Class III beta-tubulin
  • TUBB3
  • TUBB4 CFEOM3A
  • Tubulin Beta 3
  • tubulin beta-3 chain
  • Tubulin beta-4 chain
  • Tubulin beta-4
  • Tubulin beta-III
  • tubulin, beta 3
  • TUJ1 antigen
  • TUJ-1 antigen

Background

beta -III Tubulin, also known as tubulin beta -4, is regarded as a neuron-specific marker. The expression of beta -III Tubulin has been suggested to be one of the earliest markers to signal commitment in primitive neuroepithelium.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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beta-III Tubulin
Antibody (TuJ-1) [NL5 ...
NL1195R
beta-III Tubulin Antibody (TuJ-1) [NL557] - Neuron-Specific
Species: All-Multi
Applications: ICC/IF

Publications for beta-III Tubulin Antibody (NL1195R)(10)

We have publications tested in 3 confirmed species: Human, Mouse, Rabbit.

We have publications tested in 3 applications: Flow Cytometry, IHC, IHC-Fr.


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Flow Cytometry
(1)
IHC
(5)
IHC-Fr
(2)
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Human
(1)
Mouse
(6)
Rabbit
(1)
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Showing Publications 1 - 10 of 10.
Publications using NL1195R Applications Species
K Yuan, J Zheng, X Shen, Y Wu, Y Han, X Jin, X Huang Sensory nerves promote corneal inflammation resolution via CGRP mediated transformation of macrophages to the M2 phenotype through the PI3K/AKT signaling pathway International immunopharmacology, 2021-12-11;102(0):108426. 2021-12-11 [PMID: 34906854] (IHC, Mouse) IHC Mouse
Y Okada, Y Zhang, L Zhang, LK Yeh, YC Wang, S Saika, CY Liu Shp2-mediated MAPK pathway regulates &deltaNp63 in epithelium to promote corneal innervation and homeostasis Lab. Invest., 2019-10-25;100(4):630-642. 2019-10-25 [PMID: 31653968] (IHC, Mouse) IHC Mouse
Mingjuan Wu, Jun Liu, Fanying Li, Shuoya Huang, Jingxin He, Yunxia Xue et al. Antibiotic-induced dysbiosis of gut microbiota impairs corneal development in postnatal mice by affecting CCR2 negative macrophage distribution Mucosal Immunology 2020-01-01 [PMID: 31434991]
JE Ahlfors, A Azimi, R El-Ayoubi, A Velumian, I Vonderwald, C Boscher, O Mihai, S Mani, M Samoilova, M Khazaei, MG Fehlings, CM Morshead Examining the fundamental biology of a novel population of directly reprogrammed human neural precursor cells Stem Cell Res Ther, 2019-06-13;10(1):166. 2019-06-13 [PMID: 31196173] (Flow Cytometry, Human) Flow Cytometry Human
Jun Liu, Mingjuan Wu, Jingxin He, Chengju Xiao, Yunxia Xue, Ting Fu et al. Antibiotic-Induced Dysbiosis of Gut Microbiota Impairs Corneal Nerve Regeneration by Affecting CCR2-Negative Macrophage Distribution The American Journal of Pathology 2019-12-01 [PMID: 30470496]
DL Harris, T Yamaguchi, P Hamrah A Novel Murine Model of Radiation Keratopathy Invest. Ophthalmol. Vis. Sci., 2018-08-01;59(10):3889-3896. 2018-08-01 [PMID: 30073349] (IHC-Fr, Mouse) IHC-Fr Mouse
EI Paschalis, F Lei, C Zhou, V Kapoulea, A Thanos, R Dana, D Vavvas, J Chodosh, CH Dohlman The Role of Microglia and Peripheral Monocytes in Retinal Damage Following Corneal Chemical Injury Am. J. Pathol., 2018-04-06;0(0):. 2018-04-06 [PMID: 29630857] (IHC, Mouse) IHC Mouse
H Wang, C Xiao, D Dong, C Lin, Y Xue, J Liu, M Wu, J He, T Fu, H Pan, X Jiao, D Lu, Z Li Epothilone B Speeds Corneal Nerve Regrowth and Functional Recovery through Microtubule Stabilization and Increased Nerve Beading Sci Rep, 2018-02-08;8(1):2647. 2018-02-08 [PMID: 29422528] (IHC, Mouse) IHC Mouse
EI Paschalis, C Zhou, F Lei, N Scott, V Kapoulea, MC Robert, D Vavvas, R Dana, J Chodosh, CH Dohlman Mechanisms of Retinal Damage after Ocular Alkali Burns Am. J. Pathol., 2017-04-13;0(0):. 2017-04-13 [PMID: 28412300] (IHC, Rabbit) IHC Rabbit
Intranasal delivery of nanomicelle curcumin promotes corneal epithelial wound healing in streptozotocin-induced diabetic mice Sci Rep, 2016-07-11;6(0):29753. 2016-07-11 [PMID: 27405815] (IHC-Fr, Mouse) IHC-Fr Mouse

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Secondary Antibodies

 

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Beta tubulin III, also known as Tuj-1, is a class III member of the beta tubulin protein family. Beta tubulins are one of two structural components that form our microtubule network. While general tubulins play a role in a wide range of cellular pr...  Read full blog post.
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Bioinformatics

Gene Symbol TUBB3