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ERCC1 Antibody (8F1) - BSA Free

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Immunohistochemistry-Paraffin: ERCC1 Antibody (8F1) [NB500-704] - Cyto-histologic comparison of anti-ERCC1 immunoreactivity using the protocol 8F1 CC1-mono (top) or 8F1 H2-60 (bottom). Left: Pleural effusion sediment of ...read more

Product Details

Summary
Reactivity Hu, Rt, ChHaSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
8F1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

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Reviews & Publications
Protocols & FAQs
Support & Research

ERCC1 Antibody (8F1) - BSA Free Summary

Immunogen
Full length recombinant human ERCC1. [UniProt# P07992]
Localization
Nuclear.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
ERCC1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:200
  • Western Blot 1:100-1:2000
Application Notes
IHC: In an ABC method, we suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Antibody can be used in Immunocytochemistry/Immunoflourescence as reported in the literature (PMID: 21961533)Western Blot was reported in scientific literature.
Control
Human Tonsil Whole Tissue Lysate (Adult Whole Normal)
ERCC1 Overexpression Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB500-704 in the following applications:

Publications
Read Publications using
NB500-704 in the following applications:

Reactivity Notes

Human, rat. Chinese Hamster reactivity reported in scientific literature (PMID: 17962301) Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for ERCC1 Antibody (8F1) - BSA Free

  • COFS4
  • DNA excision repair protein ERCC-1
  • ERCC1
  • excision repair cross-complementing rodent repair deficiency, complementationgroup 1 (includes overlapping antisense sequence)
  • RAD10
  • UV20

Background

ERCC1, also known as RAD10, COFS4 and UV20, is a gene that functions in the nucleotide excision repair pathway. Specifically, ERCC1 is required for the repair of DNA lesions induced by UV light or formed by electrophilic compounds. ERCC1 is a heterodimer composed of ERCC1 and XPF/ERRC4 and is located in the nucleus. Mutations in ERCC1 result in cerebrooculofacio skeletal syndrome, as well as carcinogenesis. Recent research on DNA repair suggests that DNA damage may drive accelerated bone aging (PMID: 23281008), and that defaults in DNA repair mechanisms are prevalent in many lung cancers (PMID: 23276504).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Recent Reviews

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Verified Customer
12/14/2011
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol ERCC1
Entrez
Uniprot