Reactivity | Hu, Rt, ChHaSpecies Glossary |
Applications | WB, ICC/IF, IHC |
Clone | 8F1 |
Clonality | Monoclonal |
Host | Mouse |
Conjugate | Unconjugated |
Format | BSA Free |
Concentration | 1.0 mg/ml |
Immunogen | Full length recombinant human ERCC1. [UniProt# P07992] |
Localization | Nuclear. |
Isotype | IgG2b |
Clonality | Monoclonal |
Host | Mouse |
Gene | ERCC1 |
Purity | Protein G purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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Application Notes | IHC: In an ABC method, we suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Antibody can be used in Immunocytochemistry/Immunoflourescence as reported in the literature (PMID: 21961533)Western Blot was reported in scientific literature. |
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Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer | PBS |
Preservative | 0.02% Sodium Azide |
Concentration | 1.0 mg/ml |
Purity | Protein G purified |
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