The Beta III tubulin protein is abundantly present in both the central and peripheral nervous systems (CNS and PNS), where it is predominantly expressed during fetal and postnatal development. In cerebellar and sympathoadrenal neurogenesis, Beta III distribution is neuron-associated and present in distinct temporal-spatial gradients that are dictated by the regional neuroepithelia of origin. In addition, CNS subventricular zones consisting of neuronal and glial precursor cells exhibit transient beta III expression, where it may enable the identification of presumptive neurons derived from embryonic stem cells. In contrast, Beta III distribution is almost exclusively neuron-specific in adult tissues. Researchers published in Cell share their findings that the receptor tyrosine kinase Ret is required for motor axon attraction by integrating diffusible- and contact-axon guidance signaling in a hierarchical GPI-receptor signaling system1.
Immunocytochemistry/Immunofluorescence: Tubulin Beta 3 Antibody
Their experiments with the Beta III tubulin antibody allowed them to construct a network from combinatorial components. Rudenko’s group from NIH used the Beta III tubulin antibody in their aging studies centered on the role of autosomal-dominant missense mutations on the multidomain leucine-rich repeat kinase 2 (LRRK2) that contains both kinase and ATPase activity2. They determined that the G2385R variant is a partial loss-of-function mutant in Parkinson's disease (PD) that appears to be kinase-activating-independent. Immunoblotting and immunohistochemistry studies with the Beta III tubulin antibody performed in the Yoo lab focused on the chronic effects of pyridoxine vitamin B6 on forebrain ischemic damage and subsequent neuroblast differentiation3. Using the Beta III tubulin antibody, Ghod’s group examined the role of the anti-inflammatory drug dimethylfumarate (DMF) on proliferation, apoptosis, and differentiation in glioma models4. They found DMF to be promising as a new treatment modality for brain tumors. The Beta III tubulin antibody allowed Kong et al to monitor the effects of chronic exposure to low doses of methylmercury on adult rat somatosensory cortexes5. Their comparative proteomic studies demonstrated the induction of a state of metabolic deficit. Novus Biologicals offers Tubulin Beta 3 reagents for your research needs including:
PMIDs
