![Western Blot: beta-Catenin Antibody (CTNNB1/1509) [NBP2-53366] - Breast cancer cell lysates were probed with Mouse anti-Human Beta-Catenin antibody (#NBP2-53366-20ug) at 0.5 ug/mL, followed by 1:5,000 anti-Mouse secondary antibody. Image is courtesy of customer review.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CTNNB1-1509-Western-Blot-NBP2-53366-img0005.jpg "Western Blot: beta-Catenin Antibody (CTNNB1/1509) [NBP2-53366] - Breast cancer cell lysates were probed with Mouse anti-Human Beta-Catenin antibody (#NBP2-53366-20ug) at 0.5 ug/mL, followed by 1:5,000 anti-Mouse secondary antibody. Image is courtesy of customer review.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, IHC |
| Clone | CTNNB1/1509 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54540) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Recombinant human beta-Catenin protein fragment (Uniprot: P35222) |
| Localization | Cell surface and Cytoplasmic |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CTNNB1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 0.1-0.2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
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| Theoretical MW | 92 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Reviewed Applications |
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| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
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| Images | Ratings | Applications | Species | Date | Details | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Enlarge |
reviewed by:
Verified Customer |
WB | Human | 04/03/2017 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
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