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beta-Catenin Antibody (196621) [Unconjugated]

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beta-Catenin was detected in immersion fixed paraffin-embedded sections of human colon cancer using Mouse Anti-Human beta-Catenin Monoclonal Antibody (Catalog # MAB1329) at 15 µg/mL at 37° Celsius for 2 minutes. ...read more
Western blot shows lysates of Huh-7 human hepatoma cell line, C6 rat glioma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat beta ...read more
beta -Catenin was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human/Mouse/Rat beta -Catenin Monoclonal Antibody (Catalog # MAB1329) at 1.7 µg/mL overnight at 4 °C. ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, mIF
Clone
196621
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

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beta-Catenin Antibody (196621) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human beta -Catenin
Ala2-Leu781
Accession # P35222
Specificity
Detects rh beta -Catenin and endogenous human, mouse and rat beta -Catenin in Western blots.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CTNNB1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Multiplex Immunofluorescence 15 ug/mL
  • Western Blot 2 ug/mL
Publications
Read Publications using
MAB1329 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for beta-Catenin Antibody (196621) [Unconjugated]

  • bCatenin
  • beta 1 (88kD)
  • beta-Catenin
  • catenin (cadherin-associated protein), beta 1, 88kDa
  • catenin beta-1
  • CTNNB
  • CTNNB1
  • DKFZp686D02253
  • FLJ25606
  • FLJ37923

Background

beta -Catenin is a cadherin-associated protein that is involved in the regulation of cell adhesion. It also acts as a transcriptional co-activator in the nucleus and is involved in the canonical Wnt signal transduction pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for beta-Catenin Antibody (MAB1329)(5)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: ICC, IHC-P, Western Blot.


Filter By Application
ICC
(2)
IHC-P
(1)
Western Blot
(2)
All Applications
Filter By Species
Human
(1)
Mouse
(2)
Rat
(1)
All Species
Showing Publications 1 - 5 of 5.
Publications using MAB1329 Applications Species
V Veschi, LR Mangiapane, A Nicotra, S Di Franco, E Scavo, T Apuzzo, DS Sardina, M Fiori, A Benfante, ML Colorito, G Cocorullo, F Giuliante, C Cipolla, G Pistone, MR Bongiorno, A Rizzo, CM Tate, X Wu, S Rowlinson, LF Stancato, M Todaro, R De Maria, G Stassi Targeting chemoresistant colorectal cancer via systemic administration of a BMP7 variant Oncogene, 2019-10-07;0(0):. 2019-10-07 [PMID: 31591478] (ICC, Human) ICC Human
Jade E. Jones, Susheel Bhanu Busi, Jonathan B. Mitchem, James M. Amos-Landgraf, Michael R. Lewis Evaluation of a Tumor-Targeting, Near-Infrared Fluorescent Peptide for Early Detection and Endoscopic Resection of Polyps in a Rat Model of Colorectal Cancer Mol Imaging 8/2/2018 [PMID: 30064304]
Peng J, Lai Z, Fang Z, Xing S, Hui K, Hao C, Jin Q, Qi Z, Shen W, Dong Q, Bing Z, Fu D Dimethyloxalylglycine prevents bone loss in ovariectomized C57BL/6J mice through enhanced angiogenesis and osteogenesis. PLoS ONE, 2014-11-13;9(11):e112744. 2014-11-13 [PMID: 25394221] (Western Blot, Mouse) Western Blot Mouse
Wu , Yi Ju, Fang , Yen Hsin, Chi , Hsiang C, Chang , Li Chiun, Chung , Shih Yin, Huang , Wei Chie, Wang , Xiao Wen, Lee , Kuan Wei, Chen , Shen Lia Insulin and LiCl synergistically rescue myogenic differentiation of FoxO1 over-expressed myoblasts. PLoS ONE, 2014-02-13;9(2):e88450. 2014-02-13 [PMID: 24551104] (Western Blot, Mouse) Western Blot Mouse
Liu YT, Shang D, Akatsuka S, Ohara H, Dutta KK, Mizushima K, Naito Y, Yoshikawa T, Izumiya M, Abe K, Nakagama H, Noguchi N, Toyokuni S Chronic oxidative stress causes amplification and overexpression of ptprz1 protein tyrosine phosphatase to activate beta-catenin pathway. Am. J. Pathol., 2007-11-30;171(6):1978-88. 2007-11-30 [PMID: 18055543] (ICC, IHC-P, Rat) ICC, IHC-P Rat

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for beta-Catenin Antibody (MAB1329) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CTNNB1
Uniprot