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beta-Catenin Antibody (12F7) [DyLight 680]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Av-Du, Ch, PmSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IP
Clone
12F7
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 680

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-54467AF350
Alexa Fluor 405
NBP1-54467AF405
Alexa Fluor 488
NBP1-54467AF488
Alexa Fluor 532
NBP1-54467AF532
Alexa Fluor 594
NBP1-54467AF594
Alexa Fluor 647
NBP1-54467AF647
Alexa Fluor 700
NBP1-54467AF700
Alexa Fluor 750
NBP1-54467AF750
Biotin
NBP1-54467B
DyLight 350
NBP1-54467UV
DyLight 405
NBP1-54467V
DyLight 488
NBP1-54467G
DyLight 550
NBP1-54467R
DyLight 594
NBP1-54467DL594
DyLight 650
NBP1-54467C
DyLight 755
NBP1-54467IR
FITC
NBP1-54467F
HRP
NBP1-54467H
Janelia Fluor 549
NBP1-54467JF549
Janelia Fluor 646
NBP1-54467JF646
Unconjugated
NBP1-54467
mFluor Violet 450 SE
NBP1-54467MFV450
mFluor Violet 500 SE
NBP1-54467MFV500
mFluor Violet 610 SE
NBP1-54467MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP1-54467
BSA Free
NBP1-54467
BSA Free
NBP1-54467SS
Azide and BSA Free
NBP2-80596
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beta-Catenin Antibody (12F7) [DyLight 680] Summary

Immunogen
Recombinant chicken beta Catenin fused to maltose binding protein. [UniProt# O42486]
Localization
Nucleus, Cytoplasm, Cytoskeleton, Adherens junction, Centrosome, Spindle pole.
Marker
Epithelial Cell Marker, Adherens Junction Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CTNNB1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Simple Western
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Publications
Read Publication using
NBP1-54467FR in the following applications:

  • ICC
    1 publication

Reactivity Notes

Use in Duck reported in scientific literature (PMID:32692763).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for beta-Catenin Antibody (12F7) [DyLight 680]

  • bCatenin
  • beta 1 (88kD)
  • beta-Catenin
  • catenin (cadherin-associated protein), beta 1, 88kDa
  • catenin beta-1
  • CTNNB
  • CTNNB1
  • DKFZp686D02253
  • FLJ25606
  • FLJ37923

Background

The protein encoded by the beta Catenin gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs arenecessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion betweencells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contactinhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this proteinbinds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this geneare a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Three transcript variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-54467FR
beta-Catenin Antibody (12F7) [DyLight 680]
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Applications: WB, Simple Western, Flow, ICC/IF, IHC, IP

Publications for beta-Catenin Antibody (NBP1-54467FR)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC.


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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CTNNB1