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beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody - BSA Free

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Immunocytochemistry/Immunofluorescence: beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody [NBP1-78982] - Antibody was tested in U2OS cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunocytochemistry/ Immunofluorescence: beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody [NBP1-78982] - Antibody was tested in U2OS cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) ...read more
Immunohistochemistry: beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody [NBP1-78982] - Analysis of CD57 in mouse brain using DAB with hematoxylin counterstain.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.18 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-78982AF350
Alexa Fluor 405
NBP1-78982AF405
Alexa Fluor 488
NBP1-78982AF488
Alexa Fluor 532
NBP1-78982AF532
Alexa Fluor 594
NBP1-78982AF594
Alexa Fluor 647
NBP1-78982AF647
Alexa Fluor 700
NBP1-78982AF700
Alexa Fluor 750
NBP1-78982AF750
Allophycocyanin
NBP1-78982APC
Biotin
NBP1-78982B
CoraFluor 1
NBP1-78982CL1
DyLight 350
NBP1-78982UV
DyLight 405
NBP1-78982V
DyLight 488
NBP1-78982G
DyLight 550
NBP1-78982R
DyLight 594
NBP1-78982DL594
DyLight 650
NBP1-78982C
DyLight 680
NBP1-78982FR
DyLight 755
NBP1-78982IR
FITC
NBP1-78982F
HRP
NBP1-78982H
Janelia Fluor 525
NBP1-78982JF525
Janelia Fluor 549
NBP1-78982JF549
Janelia Fluor 585
NBP1-78982JF585
Janelia Fluor 635
NBP1-78982JF635
Janelia Fluor 646
NBP1-78982JF646
Janelia Fluor 669
NBP1-78982JF669
PE
NBP1-78982PE
PerCP
NBP1-78982PCP
mFluor Violet 450 SE
NBP1-78982MFV450
mFluor Violet 500 SE
NBP1-78982MFV500
mFluor Violet 610 SE
NBP1-78982MFV610
View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP1-78982SS
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beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody - BSA Free Summary

Immunogen
A synthetic peptide made to a C-terminal portion of the human B3GAT1 protein (between residues 250-300) [UniProt Q9P2W7]
Localization
Golgi apparatus membrane; Single-pass type II membrane protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
B3GAT1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:600
  • Immunohistochemistry-Paraffin 1:600
Application Notes
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.18 mg/ml
Purity
Immunogen affinity purified

Alternate Names for beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody - BSA Free

  • B3GAT1
  • beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P)
  • Beta-1,3-glucuronyltransferase 1
  • CD57
  • EC 2.4.1.135
  • galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1
  • GLCATP
  • GlcAT-P
  • GLCATPGLCUATP
  • GLCUATP
  • glcUAT-P
  • Glucuronosyltransferase P
  • HNK1
  • LEU7
  • NK1
  • NK-1
  • UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase

Background

CD57 (also known as beta-1,3-glucuronyltransferase 1, Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, Glucuronosyltransferase P, Human Natural Killer 1, B3GAT1, NK1, HNK1, LEU7, NK-1, GLCUATP, and GLCATP) is a member of the glucuronyltransferase gene family. The enzymes produced by the members of this family recognize non-reducing terminal sugars and their anomeric linkages by exhibiting strong acceptor specificity. CD57 is also involved in the biosynthesis of glycosaminoglycan and L2/HNK-1 carbohydrate epitopes on glycoproteins. CD57 is expressed mainly in the brain and several alternate transcriptional splice variants have been characterized. In cancer research, CD57 positive lymphocytes have been used successfully in monitoring the progression of various cancers (PMID: 21491089). HNK1 is also involved in synaptic plasticity and neurodevelopment (PMID: 20950796).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Bioinformatics

Gene Symbol B3GAT1
Entrez
Uniprot