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Neurokinin B Antibody - BSA Free

Images

 
Biological Strategies: Immunohistochemistry: Neurokinin B Antibody - BSA Free [NB300-201] - Immunofluorescence photomicrograph showing that the expression of Neurokinin B in the arcuate nucleus (ARC). Scale bars: ...read more
Immunohistochemistry-Paraffin: Neurokinin B Antibody [NB300-201] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse brain using Neurokinin B antibody at 1:200 dilution. The antibody depicted an ...read more
Immunohistochemistry-Paraffin: Neurokinin B Antibody [NB300-201] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse brain using Neurokinin B antibody at 1:100 dilution. The antibody showed a ...read more
Immunohistochemistry: Neurokinin B Antibody [NB300-201] - IF analysis of Neurokinin B in mouse hypothalamus. Image courtesy of an anonymous customer review.
Immunohistochemistry: Neurokinin B Antibody [NB300-201] - Detection of Neurokinin B in rat brain tissue (Alexa 488 stain), using NB 300-201.
Immunocytochemistry/ Immunofluorescence: Neurokinin B Antibody - BSA Free [NB300-201] - Troxerutin reduced the expression of Kisspepetin1 & Neurokinin B in the ARC. a Immunofluorescence photomicrograph showing that the ...read more
Immunohistochemistry: Neurokinin B Antibody - BSA Free [NB300-201] - Characterization of Kiss1-null mice.(A-C) Immunofluorescence for kisspeptin (A,B) & NKB (C) in the ARN of wild-type (A) & Kiss1-null (B,C) female ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

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Neurokinin B Antibody - BSA Free Summary

Immunogen
A synthetic peptide made to an internal sequence of mouse proNeurokinin B (~93% homology to the rat sequence). [UniProt# P55099]
Localization
Secreted
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TAC3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:500-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:100-1:2000
Application Notes
This Neurokinin B antibody is useful for Immunocytochemistry/Immunofluorescence and Immunohistochemistry on paraffin-embedded and frozen sections. This antibody has been used on human hypothalamus and rat brain sections. In IHC, positive staining of the arcuate nucleus is seen. There has been customer feedback that this antibody works in Western blot; please note that this is a secreted protein.
Control
Neurokinin B Overexpression Lysate
Control Peptide
Neurokinin B Peptide (NB300-201PEP)
Reviewed Applications
Read 2 Reviews rated 4
using
NB300-201 in the following applications:

Publications
Read Publications using
NB300-201 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Neurokinin B Antibody - BSA Free

  • gamma tachykinin 3
  • neurokinin beta
  • neurokinin B-like
  • neuromedin K
  • preprotachykinin B
  • PRO1155
  • tachykinin 3
  • tachykinin-3
  • ZNEUROK1NKNBNKB

Background

Neurokinin B (NKB) belongs to tachykinin family of peptides that also includes substance P, NKA, neuropeptide K, neuropeptide-gamma, and hemokinin-1, and NKB is the only tachykinin synthesized from preprotachykinin-B gene (designated as TAC3 in mammals, except for rodents where it is named Tac2). Tachykinins are characterized by a common carboxyl-terminal amino acid sequence of FXGLM-NH2 , X being a hydrophobic residue and are active peptides which stimulates neurons, evoke behavioral responses, are potent vasodilators and secretagogues (secretion stimulators), and contract directly or indirectly smooth muscles. NKB is the cleaved product of neurokinin B-like protein Zneurok1 (also called preprotachykinin-B or tachykinin-3) and act as an endogenous agonist ligand for its receptor, the NK3 receptor (NKB receptor). NKB signaling has been reported to play critical role in pre-eclampsia which is a potentially life threatening disease and is essential to neuroprotection in neurodegenerative diseases as well as to normal reproductive development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Neurokinin B Antibody (NB300-201)(25)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: ICC/IF, IF/IHC, IHC-Fr, WB.


Filter By Application
ICC/IF
(1)
IF/IHC
(6)
IHC-Fr
(7)
WB
(3)
All Applications
Filter By Species
Human
(2)
Mouse
(5)
Rat
(12)
All Species
Showing Publications 1 - 10 of 25. Show All 25 Publications.
Publications using NB300-201 Applications Species
L Naulé, A Mancini, SA Pereira, BM Gassaway, JR Lydeard, JC Magnotto, HK Kim, J Liang, C Matos, SP Gygi, FT Merkle, RS Carroll, AP Abreu, UB Kaiser MKRN3 inhibits puberty onset via interaction with IGF2BP1 and regulation of hypothalamic plasticity JCI Insight, 2023-04-24;8(8):. 2023-04-24 [PMID: 37092553]
Roberts SA, NaulE L, Chouman S et al. Hypothalamic Overexpression of Makorin Ring Finger Protein 3 Results in Delayed Puberty in Female Mice Endocrinology 2022-08-17 [PMID: 35974456]
Senft RA, Freret ME, Sturrock N, Dymecki SM Neurochemically and hodologically distinct ascending VGLUT3 versus serotonin subsystems comprise the r2-Pet1 median raphe The Journal of neuroscience : the official journal of the Society for Neuroscience 2021-02-05 [PMID: 33547164]
Liu, X, Yeo, S H Et al. Highly redundant neuropeptide volume co-transmission underlying episodic activation of the GnRH neuron dendron. Elife 2021-01-19 [PMID: 33464205] (WB, Human) WB Human
Chen S, Gao XF, Zhou Y et al. A spinal neural circuitry for converting touch to itch sensation Nat Commun 2020-10-08 [PMID: 33033265] (IHC-Fr, Mouse) IHC-Fr Mouse
Gao Z, Ma X, et al. Troxerutin protects against DHT-induced polycystic ovary syndrome in rats. J Ovarian Res 2020-09-13 [PMID: 32921318] (IF/IHC, Rat) IF/IHC Rat
Dees WL, Hiney JK, Srivastava VK Regulation of prepubertal dynorphin secretion in the medial basal hypothalamus of the female rat J. Neuroendocrinol. 2019-12-01 [PMID: 31715027] (WB, Rat) WB Rat
Sakata D, Uruno T et al. Selective role of neurokinin B in IL-31-induced itch response in mice. J Allergy Clin Immunol 2019-01-10 [PMID: 31405606] (IF/IHC, Mouse) IF/IHC Mouse
Fergani C, Leon S, Padilla SL et al. NKB signaling in the posterodorsal medial amygdala stimulates gonadotropin release in a kisspeptin-independent manner in female mice Elife 2018-12-19 [PMID: 30565563] (IHC-Fr, Mouse) IHC-Fr Mouse
Srivastava Vinod K, Hiney Jill K, Dees William L et al. Alcohol Delays the Onset of Puberty in the Female Rat by Altering Key Hypothalamic Events. Alcoholism, Clinical and Experimental Research 2018-07-01 [PMID: 29689132] (WB, Rat) WB Rat
Show All 25 Publications.

Reviews for Neurokinin B Antibody (NB300-201) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Rat.

Reviews using NB300-201:
Filter by Applications
IHC
(1)
IF
(1)
All Applications
Filter by Species
Mouse
(1)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry Neurokinin B NB300-201
Enlarge
4
reviewed by:
Andree Lessard
IHC Rat 06/22/2012
View

Summary

ApplicationImmunohistochemistry
Sample Testedrat tissue fixed with PFA and acrolein
SpeciesRat
Immunofluorescence Neurokinin B NB300-201
Enlarge
4
reviewed by:
Christina Merkley
IF Mouse 05/15/2012
View

Summary

ApplicationImmunofluorescence
Sample TestedMouse hypothalamus
SpeciesMouse
LotA3

Product General Protocols

View specific protocols for Neurokinin B Antibody (NB300-201): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Neurokinin B Antibody (NB300-201). (Showing 1 - 1 of 1 FAQs).

  1. Has NB300-201 been tested by IHC on frozen of paraffin embedded tissue section?
    • This antibody is suitable for use on both frozen and paraffin-embedded tissues.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on Neurokinin B

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

Andree Lessard
06/22/2012
Application: IHC
Species: Rat

Christina Merkley
05/15/2012
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol TAC3
Entrez
Uniprot