I was wondering about the Rabbit polyclonal antibody to Neurokinin-3-receptor, catalog #NB300-102. I am wondering about its information on the immunogen. You state that, NB300-102 was raised against a synthetic peptide at the c-terminus of rat Nk-3 conjugated to bovine thyroglobulin. I was wondering if you could tell me exactly which amino acids that this does correspond to? I am interested in comparing it to the Rabbit anti-Nk3R used for western blot, catalog#R-103-100, which states that it corresponds to the aa:434-452 of the absolute c-terminal of rat Nk3R protein.

Name: NK3R/TACR3/Neurokinin B Receptor Antibody
Brand: Novus Biologicals
SKU: NB300-102
Price: 399 USD

The immunogen for this antibody is between amino acids 375 and 452 of rat NK3R. Any further information regarding the immunogen is considered proprietary.

NK3R/TACR3/Neurokinin B Receptor Antibody (NB300-102): Novus Biologicals

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Mu, Rt, Gp, Sh, Hu(-)Species Glossary
Applications	ICC/IF, IHC, IHC, WB (-)
Clonality	Polyclonal
Host	Rabbit
Conjugate	Unconjugated

Immunogen	A synthetic peptide at the C-terminus of rat Neurokinin B Receptor, conjugated to bovine thyroglobulin. [UniProt# P16177]
Localization	In immunohistochemical experiments staining of the neuronal cell bodies and fibers is seen and staining is believed to be localized to the plasma membrane.
Specificity	NB300-102 is specific to the NK-3 receptor. Specificity was verified by the localization of immunohistochemical staining as well as pre-absorption studies with NK-3 peptide. (1)
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	TACR3
Purity	Unpurified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer	Whole antisera
Preservative	0.1% Sodium Azide
Purity	Unpurified

Publications using NB300-102	Applications	Species
Kodani Y, Kawata M, Suga H et al. Characterization of Hypothalamic MCH Neuron Development in a 3D Differentiation System of Mouse Embryonic Stem Cells eNeuro [PMID: 35437265]
Chen SL, Liu YG, Zhou YT et al. Morphological and Functional Evidence of Sexual Dimorphism in the Retrochiasmatic Area Population of NK3R-containing Neurons in Sheep Thesis (IHC-FrFl, Sheep)	IHC-FrFl	Sheep
Persephone A Miller, Jesukhogie G Williams-Ikhenoba, Aditi S Sankhe, Brendan H Hoffe, Melissa J Chee Neuroanatomical, electrophysiological, and morphological characterization of melanin-concentrating hormone cells coexpressing cocaine- and amphetamine-regulated transcript. The Journal of comparative neurology 2024-02-14 [PMID: 38335050]
Edvinsson JC, Reducha PV, Sheykhzade M et al. Neurokinins and their receptors in the rat trigeminal system: Differential localization and release with implications for migraine pain Molecular pain 2021-12-13 [PMID: 34898306] (IHC-Fr, Rat)	IHC-Fr	Rat
Ashley N Lindo, Jennifer F Thorson, Michelle N Bedenbaugh, Richard B McCosh, Justin A Lopez, Samantha A Young, Lanny J Meadows, Elizabeth C Bowdridge, Chrysanthi Fergani, Bradley A Freking, Michael N Lehman, Stanley M Hileman, Clay A Lents Localization of kisspeptin, NKB, and NK3R in the hypothalamus of gilts treated with the progestin altrenogest. Biology of reproduction 2022-01-03 [PMID: 34037695]
Lopez, J A, Bowdridge, E C Et al. Morphological and functional evidence for sexual dimorphism in neurokinin B signalling in the retrochiasmatic area of sheep. J Neuroendocrinol 2020-07-01 [PMID: 32572994] (IF/IHC, Mouse)	IF/IHC	Mouse
Yin C, Ishii T, Kaneda M Two Types of Cl Transporters Contribute to the Regulation of Intracellular Cl Concentrations in ON- and OFF-type Bipolar Cells in the Mouse Retina Neuroscience 2020-06-10 [PMID: 32531472] (IF/IHC, Mouse)	IF/IHC	Mouse
Goodman R L, He W et al. Evidence That the LH Surge in Ewes Involves Both Neurokinin B-Dependent and -Independent Actions of Kisspeptin. Endocrinology 2019-01-12 [PMID: 31599937] (IF/IHC, Sheep)	IF/IHC	Sheep
Sakata D, Uruno T et al. Selective role of neurokinin B in IL-31-induced itch response in mice. J Allergy Clin Immunol 2019-01-10 [PMID: 31405606] (IF/IHC, Mouse)	IF/IHC	Mouse
Fergani C, Leon S, Padilla SL et al. NKB signaling in the posterodorsal medial amygdala stimulates gonadotropin release in a kisspeptin-independent manner in female mice Elife 2018-12-19 [PMID: 30565563] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Hsu YW, Morton G, Guy EG et al. Dorsal Medial Habenula Regulation of Mood-Related Behaviors and Primary Reinforcement by Tachykinin-Expressing Habenula Neurons eNeuro 2016-08-02 [PMID: 27482535]
Ahn T, Fergani C, Coolen LM et al. Prenatal Testosterone Excess Decreases Neurokinin 3 Receptor Immunoreactivity within the Arcuate Nucleus KNDy cell population. J. Neuroendocrinol. 2014-12-10 [PMID: 25496429] (IHC-FrFl, Sheep) Details: NK3R antibody used for IHC-FrFl (IHC on free floating sections) application at 1 : 10,000 dilution in experiments involving control and prenatal testosterone-treated Suffolk ewes (see full text for detailed protocols and staining data).	IHC-FrFl	Sheep
Nuhn JS, Fuerst PG. Developmental localization of adhesion and scaffolding proteins at the cone synapse. Gene Expr. Patterns. 2014-08-28 [PMID: 25176525]
Sawai N, Iijima N, Ozawa H, Matsuzaki T. Neurokinin B- and kisspeptin-positive fibers as well as tuberoinfundibular dopaminergic neurons directly innervate periventricular hypophyseal dopaminergic neurons in rats and mice. Neurosci. Res. 2014-05-09 [PMID: 24815059] (IHC-Fr, Rat)	IHC-Fr	Rat
de Andrade GB, Long SS, Fleming H et al. DSCAM localization and function at the mouse cone synapse. J Comp. Neurol. 2014-01-30 [PMID: 24477985] (IHC-Fr, Mouse) Details: Neurokinin B Receptor antibody used at 1:2000 dilution for IHC-Fr staining of type 1 and 2 cone bipolar cells in mice. Figure 2 A-D - adult wild-type and Dscam2J/2J retina section. Figure 14 O - sections of DscamFD/FD retina.	IHC-Fr	Mouse
Hether S, Misono K, Lessard A. The neurokinin-3 receptor (NK3R) antagonist SB222200 prevents the apomorphine-evoked surface but not nuclear NK3R redistribution in dopaminergic neurons of the rat ventral tegmental area. Neuroscience 2013-05-11 [PMID: 23673279] (Rat)		Rat
Nivison-Smith L, Sun D, Fletcher EL et al. Mapping kainate activation of inner neurons in the rat retina. J Comp Neurol 2013-08-01 [PMID: 23348566] (Rat)		Rat
Sawai N, Iijima N, Takumi K et al. Immunofluorescent histochemical and ultrastructural studies on the innervation of kisspeptin/neurokinin B neurons to tuberoinfundibular dopaminergic neurons in the arcuate nucleus of rats Neurosci Res 2012-06-09 [PMID: 22691459] (IF/IHC, ICC/IF, Rat)	IF/IHC, ICC/IF	Rat
Misono K, Lessard A. Apomorphine-evoked redistribution of neurokinin-3 receptors in dopaminergic dendrites and neuronal nuclei of the rat ventral tegmental area. Neuroscience;203:27-38. 2012-02-17 [PMID: 22200547]
Krajewski SJ, Burke MC, Anderson MJ et al. Forebrain projections of arcuate neurokinin B neurons demonstrated by anterograde tract-tracing and monosodium glutamate lesions in the rat. Neuroscience. 2010-03-17 [PMID: 20038444]
Amstalden M, Coolen LM, Hemmerle AM et al. Neurokinin 3 receptor immunoreactivity in the septal region, preoptic area and hypothalamus of the female sheep: colocalisation in neurokinin B cells of the arcuate nucleus but not in gonadotrophin-releasing hormone neurones. J Neuroendocrinol 2010-01-01 [PMID: 19912479] (ICC/IF, Sheep)	ICC/IF	Sheep
Le Brun I, Dufour A, Crest M et al. Differential expression of Nk1 and NK3 neurokinin receptors in neurons of the nucleus tractus solitarius and the dorsal vagal motor nucleus of the rat and mouse. Neuroscience 2008-03-03 [PMID: 18222044]
Oh EC, Khan N, Novelli E et al. Transformation of cone precursors to functional rod photoreceptors by bZIP transcription factor NRL. Proc Natl Acad Sci U S A 104(5):1679-84. 2007-01-30 [PMID: 17242361] (ICC/IF, Mouse)	ICC/IF	Mouse
Levesque M, Wallman MJ, Parent R et al. Neurokinin-1 and neurokinin-3 receptors in primate substantia nigra. Neurosci Res 2007-03-01 [PMID: 17134780]
Burke MC, Letts PA, Krajewski SJ, Rance NE. Coexpression of dynorphin and neurokinin B immunoreactivity in the rat hypothalamus: Morphologic evidence of interrelated function within the arcuate nucleus. J Comp Neurol;498(5):712-26. 2006-10-10 [PMID: 16917850]
Levesque M, Parent R, Parent A. Cellular and subcellular localization of neurokinin-1 and neurokinin-3 receptors in primate globus pallidus. Eur J Neurosci 2006-05-01 [PMID: 16817879]
Broccardo M, Linari G, Agostini S et al. Expression of NK-1 and NK-3 tachykinin receptors in pancreatic acinar cells after acute experimental pancreatitis in rats. Am J Physiol Gastrointest Liver Physiol 2006-09-01 [PMID: 16782701] (Rat)		Rat
Haley, GE, Flynn, FW. Agonist and hypertonic saline-induced trafficking of the NK3-receptors on vasopressin neurons within the paraventricular nucleus of the hypothalamus. Am J Physiol Regul Integr Comp Physiol 290(5):R1242-50. 2006-05-01 [PMID: 16357093]
Pieri M, Severini C, Amadoro G et al. AMPA receptors are modulated by tachykinins in rat cerebellum neurons. J Neurophysiol 2005-10-01 [PMID: 16160091] (ICC/IF, Rat)	ICC/IF	Rat
Krajewski SJ, Anderson MJ, Iles-Shih L Morphologic evidence that neurokinin B modulates gonadotropin-releasing hormone secretion via neurokinin 3 receptors in the rat median eminence. J Comp Neurol. 2005-08-29 [PMID: 16025449] (IHC-Fr, Rat)	IHC-Fr	Rat
Broccardo M, Ciotti MT, Linari G et al. Immunocytochemical distribution of NK-1 and NK-3 tachykinin receptors in isolated pancreatic acini of guinea pigs and rats. Peptides 2005-11-01 [PMID: 15970359]
Mukda S, Chetsawang B, Govitrapong P et al. Tachykinins and tachykinin-receptors in the rat pineal gland. Eur J Neurosci 2005-05-01 [PMID: 15926922] (Rat)		Rat
Howe, H et al. Role of neurokinin 3 receptors in supraoptic vasopressin and oxytocin neurons. J Neurosci 24(45):10103-10. 2004-11-10 [PMID: 15537880] (IF/IHC, Rat)	IF/IHC	Rat
Feigenspan, A et al. Expression of connexin36 in cone pedicles and OFF-cone bipolar cells of the mouse retina. J Neurosci 24(13):3325-34. 2004-03-31 [PMID: 15056712] (IF/IHC, ICC, Mouse)	IF/IHC, ICC	Mouse
Dick, O. The presynaptic active zone protein bassoon is essential for photoreceptor ribbon synapse formation in the retina. Neuron 37(5):775-86. 2003-03-06 [PMID: 12628168]
Yip, J et al. Localization of NK1 and NK3 receptors in guinea-pig brain. Regul Pept 98(1-2):55-62. 2001-04-02 [PMID: 11179779] (IF/IHC, Guinea Pig)	IF/IHC	Guinea Pig
Tooney, Paul A et al. Localisation of tachykinin NK1 and NK3 receptors in the human prefrontal and visual cortex. Neurosci Lett;283(3):185-8. 2000-04-14 [PMID: 10754218] (IF/IHC, Human)	IF/IHC	Human
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NK3R/TACR3/Neurokinin B Receptor Antibody

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Gene Symbol	TACR3
Entrez	6870(Human) 24808(Rat)
Uniprot	P16177(Rat)