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Human BDNF ELISA Kit (Colorimetric)

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ELISA: Human BDNF ELISA Kit (Colorimetric) [NBP2-59954]
ELISA: Human BDNF ELISA Kit (Colorimetric) [NBP2-59954]
Human BDNF ELISA Kit (Colorimetric) [NBP2-59954] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell culture supernates and tissue homogenates
Standard Curve Range
0.3125 ng/mL - 20 ng/mL
Sensitivity
0.063 ng/mL

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Human BDNF ELISA Kit (Colorimetric) Summary

Description
Assay Length: 1-5 hours
Specificity
This assay has high sensitivity and excellent specificity for detection of human BDNF. No significant cross-reactivity or interference between human BDNF and analogues was observed.
Standard Curve Range
0.3125 ng/mL - 20 ng/mL
Sensitivity
0.063 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 10
Intra-Assay
%CV < 8
Sample Volume
50 - 100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
BDNF

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Adhesive Strip (For 96 wells)
  2. Assay plate (12 x 8 coated Microwells)
  3. Biotin-antibody (100 x concentrate)
  4. Biotin-antibody Diluent
  5. HRP-avidin (100 x concentrate)
  6. HRP-avidin Diluent
  7. Instruction manual
  8. Sample Diluent
  9. Standard (Freeze dried)
  10. Stop Solution
  11. TMB Substrate
  12. Wash Buffer (25 x concentrate)

Alternate Names for Human BDNF ELISA Kit (Colorimetric)

  • Abrineurin
  • ANON2
  • BDNF
  • brain-derived neurotrophic factor
  • BULN2
  • MGC34632
  • Neurotrophin

Background

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BDNF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BDNF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BDNF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BDNF bound in the initial step. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Product PDFs

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  Manual - Human BDNF ELISA Kit (Colorimetric)
  PDF Datasheet
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Bioinformatics

Gene Symbol BDNF