![Western Blot: BANP Antibody [NB100-79784] - Detection of human and mouse SMAR1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMAR1 antibody NB100-79784 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/BANP-Antibody-Western-Blot-NB100-79784-img0013.jpg "Western Blot: BANP Antibody [NB100-79784] - Detection of human and mouse SMAR1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMAR1 antibody NB100-79784 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 425 and the C-terminus (residue 469) of human BTG3 associated nuclear protein using the numbering given in TrEMBL entry Q96GJ7 (GeneID 54971). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | BANP |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissues. |
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| Control |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunohistochemistry: BANP Antibody [NB100-79784] - Detection of human SMAR1 by immunohistochemistry. Samples: FFPE sections of human lung carcinoma. Antibodies: Affinity purified rabbit anti-SMAR1 Cat. No. NB100-79784 Lot 2 used at a dilution of 1:5,000 (0.2ug/ml) (left) and Cat. No. NB100-79784 Lot3 used at a dilution of 1:5,000 (0.2ug/ml) (right). Detection: DAB](http://images.novusbio.com/fullsize/BANP-Antibody-Immunohistochemistry-NB100-79784-img0015.jpg "Immunohistochemistry: BANP Antibody [NB100-79784] - Detection of human SMAR1 by immunohistochemistry. Samples: FFPE sections of human lung carcinoma. Antibodies: Affinity purified rabbit anti-SMAR1 Cat. No. NB100-79784 Lot 2 used at a dilution of 1:5,000 (0.2ug/ml) (left) and Cat. No. NB100-79784 Lot3 used at a dilution of 1:5,000 (0.2ug/ml) (right). Detection: DAB")
![Western Blot: BANP Antibody [NB100-79784] - Nuclear extract (NE) from HeLa cells. Antibody used at 0.1 ug/ml.](http://images.novusbio.com/fullsize/BANP-Antibody-Western-Blot-NB100-79784-img0003.jpg "Western Blot: BANP Antibody [NB100-79784] - Nuclear extract (NE) from HeLa cells. Antibody used at 0.1 ug/ml.")
![Western Blot: BANP Antibody [NB100-79784] - Detection of Human, Mouse and Rat SMAR1/BANP by Western Blot. Samples: Whole cell lysate (50 ug) from mouse 4T1, mouse CT26.WT, rat C6, HeLa, and 293T cells. Antibodies: Affinity purified rabbit anti-SMAR1/BANP antibody NB100-79784 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/BANP-Antibody-Western-Blot-NB100-79784-img0011.jpg "Western Blot: BANP Antibody [NB100-79784] - Detection of Human, Mouse and Rat SMAR1/BANP by Western Blot. Samples: Whole cell lysate (50 ug) from mouse 4T1, mouse CT26.WT, rat C6, HeLa, and 293T cells. Antibodies: Affinity purified rabbit anti-SMAR1/BANP antibody NB100-79784 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry-Paraffin: BANP Antibody [NB100-79784] - FFPE section of human breast tumor. Affinity purified rabbit anti-SMAR1/BANP used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit](http://images.novusbio.com/fullsize/BANP-Antibody-Immunohistochemistry-Paraffin-NB100-79784-img0001.jpg "Immunohistochemistry-Paraffin: BANP Antibody [NB100-79784] - FFPE section of human breast tumor. Affinity purified rabbit anti-SMAR1/BANP used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit")
![Immunohistochemistry: BANP Antibody [NB100-79784] - Sample: FFPE sections of human lung carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-fs18 SMAR1 fs20 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/BANP-Antibody-Immunohistochemistry-NB100-79784-img0009.jpg "Immunohistochemistry: BANP Antibody [NB100-79784] - Sample: FFPE sections of human lung carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-fs18 SMAR1 fs20 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB")
![Immunohistochemistry: BANP Antibody [NB100-79784] - Detection of mouse SMAR1 by immunohistochemistry. Samples: FFPE sections of mouse renal cell carcinoma. Antibodies: Affinity purified rabbit anti-SMAR1 Cat. No. NB100-79784 Lot 2 used at a dilution of 1:5,000 (0.2ug/ml) (left) and Cat. No. NB100-79784 Lot3 used at a dilution of 1:5,000 (0.2ug/ml) (right). Detection: DAB](http://images.novusbio.com/fullsize/BANP-Antibody-Immunohistochemistry-NB100-79784-img0014.jpg "Immunohistochemistry: BANP Antibody [NB100-79784] - Detection of mouse SMAR1 by immunohistochemistry. Samples: FFPE sections of mouse renal cell carcinoma. Antibodies: Affinity purified rabbit anti-SMAR1 Cat. No. NB100-79784 Lot 2 used at a dilution of 1:5,000 (0.2ug/ml) (left) and Cat. No. NB100-79784 Lot3 used at a dilution of 1:5,000 (0.2ug/ml) (right). Detection: DAB")
![Immunoprecipitation: BANP Antibody [NB100-79784] - Detection of human SMAR1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMAR1 antibody NB100-79784 (lot NB100-79784-3) used for IP at 6 ug per reaction. SMAR1 was also immunoprecipitated by a previous lot of this antibody (lot NB100-79784-2). For blotting immunoprecipitated SMAR1, NB100-79784 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/BANP-Antibody-Immunoprecipitation-NB100-79784-img0012.jpg "Immunoprecipitation: BANP Antibody [NB100-79784] - Detection of human SMAR1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMAR1 antibody NB100-79784 (lot NB100-79784-3) used for IP at 6 ug per reaction. SMAR1 was also immunoprecipitated by a previous lot of this antibody (lot NB100-79784-2). For blotting immunoprecipitated SMAR1, NB100-79784 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")