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ATF6 Antibody - BSA Free

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Immunocytochemistry/ Immunofluorescence: ATF6 Antibody [NBP1-76675] - Staining of ATF6 in human pancreas tissue with ATF6 antibody at 20 ug/ml.
Western Blot: ATF6 Antibody [NBP1-76675] - Analysis in EL4 cell lysate with antibody at (A) 0.5 and (B) 1 ug/mL.
Immunocytochemistry/ Immunofluorescence: ATF6 Antibody [NBP1-76675] - MCF7 cells using ATF6 antibody at 10 ug/ml.
Immunohistochemistry: ATF6 Antibody [NBP1-76675] - Staining of ATF6 in human breast tissue with ATF6 antibody at 5 ug/ml.
Immunocytochemistry/ Immunofluorescence: ATF6 Antibody [NBP1-76675] - Staining of ATF6 in human breast tissue with ATF6 antibody at 20 ug/ml.
Immunohistochemistry: ATF6 Antibody [NBP1-76675] - Staining of ATF6 in human pancreas tissue with ATF6 antibody at 5 ug/ml.
Immunocytochemistry/ Immunofluorescence: ATF6 Antibody [NBP1-76675] - Immunofluorescence of ATF6 in MCF7 cells with ATF6 antibody at 10 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

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ATF6 Antibody - BSA Free Summary

Immunogen
Antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human ATF6. The immunogen is located within amino acids 470 - 520 of ATF6. Amino Acid Squence: HRHEVERTKSRRMTNN
Predicted Species
Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATF6
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Western Blot 0.5 -1 ug/ml
Control
HeLa DTT Treated / Untreated Cell Lysate
Control Peptide
ATF6 Protein (NBP1-76675PEP)
Reviewed Applications
Read 1 Review rated 4
using
NBP1-76675 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for ATF6 Antibody - BSA Free

  • ACHM7
  • Activating transcription factor 6 alpha
  • activating transcription factor 6
  • atf6 a
  • ATF6 alpha
  • ATF6
  • ATF6A
  • ATF6-alpha
  • cAMP-dependent transcription factor ATF-6 alpha
  • cyclic AMP-dependent transcription factor ATF-6 alpha

Background

Disruptions of protein folding and maturation in the endoplasmic reticulum (ER) result in the activation of the unfolded protein response (UPR), an integrated cellular signaling pathway that transmits information from the ER lumen to the cytoplasm and nucleus. Activating transcription factor 6 (ATF6) as well as the ER-transmembrane protein kinases IRE1p and PERK are the major transducers of the UPR . ATF6 is an ER transmembrane protein that is normally bound to the ER chaperone GRP78, but upon ER stress is released from GRP78 and proteolytically cleaved to yield a cytosolic fragment which then migrates to the nucleus, and together with the transcription factor XBP-1, activates transcription of UPR-responsive genes. ATF6 has two isoforms (ATF6alpha and ATF6beta); only ATF6alpha is recognized by this antibody.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ATF6 Antibody (NBP1-76675) (0)

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Review for ATF6 Antibody (NBP1-76675) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NBP1-76675:
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WES protein simple
(1)
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Images Ratings Applications Species Date Details
Other ATF6 NBP1-76675
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4
reviewed by:
yann Jaudouin
WES protein simple Rat 09/14/2018
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Summary

ApplicationOther
Sample TestedCell culture cortical neuron lysate,culture cell embryonic cortical neuron lysate
SpeciesRat
Lot3681-0504

Comments

Commentsrat cortical neuron lysate 0,2 mg/ml
ATF6 1/10e dilution

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ATF6 Antibody (NBP1-76675). (Showing 1 - 2 of 2 FAQ).

  1. We are looking for an antibody specific to ATF6 alpha that will not cross-react with ATF6 beta. Would you please help confirm if any of the following are ATF6 alpha specific antibodies: NBP1-40256, NBP1-77251, NBP1-76675 & NBP1-41439?
    • The antibodies NBP1-77251 and NBP1-76675 are specific to ATF6 alpha and should not cross-react with ATF6 beta.
  2. May we ask if ATF6 is ATF6 alpha? We are looking for ATF6 alpha antibody for our customer, but found none from Novus. Only ATF6 antibodies. However, after checking http://www.uniprot.org/uniprot/P18850, I found ATF6 and ATF6 alpha seem identical. Would you please help confirm if NBP1-40256, NBP1-77251, NBP1-76675 & NBP1-41439 are all ATF6 alpha antibodies?
    • The antibodies NBP1-77251, NBP1-76675 are specific to ATF6A and should not cross-react with ATF6B.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional ATF6 Products

Research Areas for ATF6 Antibody (NBP1-76675)

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Blogs on ATF6.

ATF6 - monitoring and regulating protein folding under cellular stress
During times of cellular stress overloading of the protein folding machinery leads to the accumulation of incorrectly folded proteins. This triggers the unfolded protein response (UPR) in order to try to reestablish homeostasis or, if this fails, t...  Read full blog post.

ATF6 - a key target in alcohol-induced fatty liver disease?
The ATF6 endoplasmic reticulum (ER) stress-regulated transcription factor is constitutively expressed and plays a central role in the mammalian unfolded protein response (UPR). This fundamental pathway is responsible for balancing cellular hom...  Read full blog post.

A Key to Fight Stress: ATF6
The protein ATF6 is a constitutively expressed transcription factor that is a key mediator of the unfolded protein response (UPR) that allows mammalian cells to maintain cellular homeostasis under conditions of environmental and physiological stress. ...  Read full blog post.

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Recent Reviews

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1
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yann Jaudouin
09/14/2018
Application: WES protein simple
Species: Rat

Bioinformatics

Gene Symbol ATF6
Uniprot