| Najarro G, Brackett K, Woosley H et al. BiP/GRP78 is a pro-viral factor for diverse dsDNA viruses that promotes the survival and proliferation of cells upon KSHV infection. PLoS pathogens 2024-10-29 [PMID: 39471213] |
|
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| Kaur, B;Miglioranza Scavuzzi, B;Yang, M;Yao, J;Jia, L;Abcouwer, SF;Zacks, DN; ER Stress and Mitochondrial Perturbations Regulate Cell Death in Retinal Detachment: Exploring the Role of HIF1? Investigative ophthalmology & visual science 2024-09-03 [PMID: 39325470] |
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| Shin, GC;Lee, HM;Kim, N;Seo, SU;Kim, KP;Kim, KH; PRKCSH contributes to TNFSF resistance by extending IGF1R half-life and activation in lung cancer Experimental & molecular medicine 2024-01-10 [PMID: 38200153] |
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| Kandhari K, Kant R, Mishra N et al. Phenylarsine oxide induced corneal injury involves oxidative stress mediated unfolded protein response and ferroptotic cell death: Amelioration by NAC Free Radical Biology and Medicine 2023-11-01 [PMID: 38088264] |
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| Barroso E, Díaz M, Reguera AC et al. CHOP upregulation and dysregulation of the mature form of the SNAT2 amino acid transporter in the placentas from small for gestational age newborns Cell communication and signaling : CCS 2023-11-13 [PMID: 37957724] (WB, Human) |
WB |
Human |
| Zhang W, Yang J, Wang B et al. HRS mediates tumor immune evasion by regulating proteostasis-associated interferon pathway activation Cell reports 2023-11-28 [PMID: 37948180] (KO, Mouse) |
KO |
Mouse |
| |
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| Nancy Ahuja, Shalini Gupta, Rashmi Arora, Ella Bhagyaraj, Drishti Tiwari, Sumit Kumar, Pawan Gupta Nr1h4 and Thrb ameliorate ER stress and provide protection in the MPTP mouse model of Parkinson’s Life Science Alliance 2024-04-12 [PMID: 38609183] |
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| Md Khalilur Rahman, Balasubrahmanyam Umashankar, Hassan Choucair, Kirsi Bourget, Tristan Rawling, Michael Murray The inositol-requiring enzyme 1 (IRE1) endoplasmic reticulum stress pathway promotes MDA-MB-231 cell survival and renewal in response to the aryl-ureido fatty acid CTU. The international journal of biochemistry & cell biology 2024-04-11 [PMID: 38608921] |
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| Liu XQ, Hu T, Wu GL et al. Tanshinone IIA, the key compound in Salvia miltiorrhiza, improves cognitive impairment by upregulating A?-degrading enzymes in APP/PS1 mice International journal of biological macromolecules 2023-11-07 [PMID: 37944734] (WB, Mouse) |
WB |
Mouse |
| Kim J, Kim NH, Youn I et al. Effects of Allium macrostemon Bunge Extract on Adipose Tissue Inflammation and Hepatic Endoplasmic Reticulum Stress in High-Fat Diet-Fed and Bisphenol A-Treated C57BL/6N Mice Foods (Basel, Switzerland) 2023-10-14 [PMID: 37893670] (WB, Mouse) |
WB |
Mouse |
| Bossuat M, Rullière P, Preuilh N et al. Phenyl dialkynylcarbinols, a Bioinspired Series of Synthetic Antitumor Acetylenic Lipids Journal of medicinal chemistry 2023-10-26 [PMID: 37816126] |
|
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| Moellmann J, Krueger K, Wong DWL et al. 2,8-Dihydroxyadenine-induced nephropathy causes hexosylceramide accumulation with increased mTOR signaling, reduced levels of protective SirT3 expression and impaired renal mitochondrial function Biochimica et biophysica acta. Molecular basis of disease 2023-08-01 [PMID: 37536502] (WB, Mouse) |
WB |
Mouse |
Espina M, Di Franco N, Brañas-Navarro M et al. The GRP78-PERK axis contributes to memory and synaptic impairments in Huntington's disease R6/1 mice Neurobiology of disease 2023-07-11 [PMID: 37442396] (WB, Mouse)
Details:
Dilution: 1:1000 |
WB |
Mouse |
| Mun SH, Lee CS, Kim HJ et al. Marchf6 E3 ubiquitin ligase critically regulates endoplasmic reticulum stress, ferroptosis, and metabolic homeostasis in POMC neurons Cell reports 2023-07-25 [PMID: 37421621] (IHC, Mouse) |
IHC |
Mouse |
| Cui J, Xu H, Shi J et al. Carbonic anhydrase IX inhibitor S4 triggers release of DAMPs related to immunogenic cell death in glioma cells via endoplasmic reticulum stress pathway Cell communication and signaling : CCS 2023-06-29 [PMID: 37386564] (WB, Human) |
WB |
Human |
| Zhou F, Gao H, Shang L et al. Oridonin promotes endoplasmic reticulum stress via TP53-repressed TCF4 transactivation in colorectal cancer Journal of experimental & clinical cancer research : CR 2023-06-19 [PMID: 37337284] (WB, Human) |
WB |
Human |
| Wang W, Hawkridge AM, Ma Y et al. Ubiquitin-like protein 5 is a novel player in the UPR-PERK arm and ER stress-induced cell death The Journal of biological chemistry 2023-06-12 [PMID: 37315790] (WB, Mouse) |
WB |
Mouse |
| Varghese D, Oommen D, John A, Ali B GRP78/BiP alleviates oxLDL-induced hepatotoxicity in familial hypercholesterolemia caused by missense variants of LDLR Research Square 2023-02-24 [PMID: 37248472] (WB, Human) |
WB |
Human |
| Kiang KM, Tang W, Song Q et al. Targeting unfolded protein response using albumin-encapsulated nanoparticles attenuates temozolomide resistance in glioblastoma British journal of cancer 2023-05-01 [PMID: 36927978] (WB) |
WB |
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| Xiao J, Huang J, Jian X et al. IRE1? arm of unfolded protein response in muscle-specific TGF-? signaling-mediated regulation of muscle cell immunological properties Cellular & molecular biology letters 2023-02-27 [PMID: 36849929] (IHC, WB, Mouse) |
IHC, WB |
Mouse |
| Yu J, Shen S, Yan Y et al. Iodide Excess Inhibits Thyroid Hormone Synthesis Pathway Involving XBP1-Mediated Regulation Nutrients 2023-02-09 [PMID: 36839245] (WB, Mouse) |
WB |
Mouse |
| Drake LY, Koloko Ngassie ML, Roos BB et al. Asthmatic lung fibroblasts promote type 2 immune responses via endoplasmic reticulum stress response dependent thymic stromal lymphopoietin secretion Frontiers in physiology 2023-01-25 [PMID: 36760534] |
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| Yu D, Li J, Wang Y et al. Oridonin ameliorates acetaminophen-induced acute liver injury through ATF4/PGC-1 alpha pathway Drug development research 2022-12-26 [PMID: 36567664] |
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| Rahman MK, Umashankar B, Choucair H et al. Inclusion of the in-chain sulfur in 3-thiaCTU increases the efficiency of mitochondrial targeting and cell killing by anticancer aryl-urea fatty acids European journal of pharmacology 2023-01-15 [PMID: 36543287] (Western Blot, Human) |
Western Blot |
Human |
| Reut D, Jhee H, Caroline B et al. Neuronal IRE-1 coordinates an organism-wide cold stress response by regulating fat metabolism Cell reports 2022-11-29 [PMID: 36450261] (WB) |
WB |
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| Wang X, Xin H, Zhang C et al. GRP94 Inhabits the Immortalized Porcine Hepatic Stellate Cells Apoptosis under Endoplasmic Reticulum Stress through Modulating the Expression of IGF-1 and Ubiquitin International Journal of Molecular Sciences 2022-11-14 [PMID: 36430538] (Western Blot) |
Western Blot |
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| Madhamanchi K, Madhamanchi P, Jayalakshmi S et al. Endoplasmic reticulum stress and unfolded protein accumulation correlate to seizure recurrence in focal cortical dysplasia patients Cell stress & chaperones 2022-10-18 [PMID: 36258150] |
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| M Oro?, M Grochowski, A Jaiswar, J Legierska, K Jastrz?bsk, M Nowak-Niez, M Ko?os, W Ka?miercza, T Olesi?ski, M Lenarcik, M Cybulska, M Mikula, A ?ylicz, M Mi?czy?ska, K Zettl, JR Wi?niewski, D Walerych The molecular network of the proteasome machinery inhibition response is orchestrated by HSP70, revealing vulnerabilities in cancer cells Cell Reports, 2022-09-27;40(13):111428. 2022-09-27 [PMID: 36170818] |
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| Ming-Fo Hsu, Yoshihiro Ito, Maryam Afkarian, Fawaz G. Haj Deficiency of the Src homology phosphatase 2 in podocytes is associated with renoprotective effects in mice under hyperglycemia Cellular and molecular life sciences : CMLS 2024-04-02 [PMID: 36102977] |
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| Pritchard KA, Jing X, Teng M et al. Role of endoplasmic reticulum stress in impaired neonatal lung growth and bronchopulmonary dysplasia PloS one 2022-08-26 [PMID: 36018859] (IHC-P, WB, Human, Rat) |
IHC-P, WB |
Human, Rat |
| Peng D, Wang YX, Huang TH et al. Ligustilide Improves Cognitive Impairment via Regulating the SIRT1/IRE1 alpha/XBP1s/CHOP Pathway in Vascular Dementia Rats Oxidative medicine and cellular longevity 2022-08-16 [PMID: 36017237] (IHC-P, WB, Rat) |
IHC-P, WB |
Rat |
Jian Z, Han Y, Zhang W et al. Anti-tumor effects of dual PI3K-HDAC inhibitor CUDC-907 on activation of ROS-IRE1 alpha-JNK-mediated cytotoxic autophagy in esophageal cancer Cell & bioscience 2022-08-21 [PMID: 35989326] (IHC-P, Human)
Details:
Dilutions: 1:500 |
IHC-P |
Human |
| Soto I, Couvillion M, Hansen KG et al. Balanced mitochondrial and cytosolic translatomes underlie the biogenesis of human respiratory complexes Genome biology 2022-08-09 [PMID: 35945592] (WB) |
WB |
|
| Lei X, Lin H, Wang J et al. Mitochondrial fission induces immunoescape in solid tumors through decreasing MHC-I surface expression Nature communications 2022-07-06 [PMID: 35794100] (WB, Human) |
WB |
Human |
| Chen Y, Zhang T, Zeng S et al. Transketolase in human Muller cells is critical to resist light stress through the pentose phosphate and NRF2 pathways Redox Biology 2022-08-01 [PMID: 35779441] (WB, Human) |
WB |
Human |
| Chen JH, Wu CH, Jheng JR et al. The down-regulation of XBP1, an unfolded protein response effector, promotes acute kidney injury to chronic kidney disease transition Journal of biomedical science 2022-06-28 [PMID: 35765067] (WB, Mouse) |
WB |
Mouse |
| Bastola P, Leiserowitz GS, Chien J. Multiple Components of Protein Homeostasis Pathway Can Be Targeted to Produce Drug Synergies with VCP Inhibitors in Ovarian Cancer Cancers (Basel) 2022-06-15 [PMID: 35740614] (Western Blot) |
Western Blot |
|
| Chakraborty S, Basu A miR-451a Regulates Neuronal Apoptosis by Modulating 14-3-3 zeta-JNK Axis upon Flaviviral Infection mSphere 2022-06-21 [PMID: 35727063] (WB, Mouse) |
WB |
Mouse |
| Fanning S, Cirka H, Thies JL et al. Lipase regulation of cellular fatty acid homeostasis as a Parkinson's disease therapeutic strategy NPJ Parkinson's disease 2022-06-09 [PMID: 35680956] |
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| Grajales D, V�zquez P, Al�n R et al. Attenuation of Olanzapine-Induced Endoplasmic Reticulum Stress Improves Insulin Secretion in Pancreatic Beta Cells Metabolites 2022-05-16 [PMID: 35629947] |
|
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| Wang X, Xin H, Xing M et al. Acute Endoplasmic Reticulum Stress Induces Inflammation Reaction, Complement System Activation, and Lipid Metabolism Disorder of Piglet Livers: A Proteomic Approach Frontiers in Physiology 2022-04-13 [PMID: 35492579] (WB, Porcine) |
WB |
Porcine |
| Ghilardi AF, Yaaghubi E, Ferreira RB et al. Anticancer Agents Derived from Cyclic Thiosulfonates: Structure-Reactivity and Structure-Activity Relationships ChemMedChem 2022-07-19 [PMID: 35491396] (Western Blot) |
Western Blot |
|
| Li Q, Fan X, Lu W et al. Novel NPR2 Gene Mutations Affect Chondrocytes Function via ER Stress in Short Stature Cells 2022-04-08 [PMID: 35455946] (WB) |
WB |
|
| Komatsu A, Iida I, Nasu Y et al. Ammonia induces amyloidogenesis in astrocytes by promoting amyloid precursor protein translocation into the endoplasmic reticulum Journal of Biological Chemistry 2022-05-01 [PMID: 35427648] (Western Blot) |
Western Blot |
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| Visa, A;Alza, L;CantI, C;Herreros, J; Tetralol derivative NNC-55-0396 induces glioblastoma cell death by activating IRE1alpha, JNK1 and calcium signaling Biomedicine & Pharmacotherapy [PMID: 35367758] |
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| Lu H, Gomaa A, Wang-Bishop L et al. Unfolded Protein Response Is Activated by Aurora Kinase A in Esophageal Adenocarcinoma Cancers 2022-03-09 [PMID: 35326553] (IHC-P, Human) |
IHC-P |
Human |
| Xiaofeng Zheng, Qing Wei Calvin Ho, Minni Chua, Olga Stelmashenko, Xin Yi Yeo, Sneha Muralidharan, Federico Torta, Elaine Guo Yan Chew, Michelle Mulan Lian, Jia Nee Foo, Sangyong Jung, Sunny Hei Wong, Nguan Soon Tan, Nanwei Tong, Guy A. Rutter, Markus R. Wenk, David L. Silver, Per-Olof Berggren, Yusuf Ali Destabilization of beta Cell FIT2 by saturated fatty acids alter lipid droplet numbers and contribute to ER stress and diabetes Proceedings of the National Academy of Sciences of the United States of America 2022-03-07 [PMID: 35254894] |
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| Rudalska R, Harbig J, Snaebjornsson M et al. LXR alpha activation and Raf inhibition trigger lethal lipotoxicity in liver cancer Nature Cancer 2021-02-01 [PMID: 35122079] |
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| Yiwei Xu, Jie Chen, Jianguo Chen, Junlin Teng EI24 promotes cell adaption to ER stress by coordinating IRE1 signaling and calcium homeostasis. EMBO reports 2022-03-14 [PMID: 35005829] |
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| Srinivasan MP, Bhopale KK, Caracheo AA et al. Exposure to binge ethanol and fatty acid ethyl esters exacerbates chronic ethanol-induced pancreatic injury in hepatic alcohol dehydrogenase deficient deer mice American journal of physiology. Gastrointestinal and liver physiology 2022-01-05 [PMID: 34984929] (WB, Mouse) |
WB |
Mouse |
| Abdullah CS, Aishwarya R, Alam S et al. The molecular role of Sigmar1 in regulating mitochondrial function through mitochondrial localization in cardiomyocytes Mitochondrion 2022-01-01 [PMID: 34902622] (WB, Rat, Mouse, Human) |
WB |
Rat, Mouse, Human |
| Tanaka K, Kandori S, Sakka S Et al. ELOVL2 promotes cancer progression by inhibiting cell apoptosis in renal cell carcinoma Oncology reports 2022-02-01 [PMID: 34841437] (WB, Human) |
WB |
Human |
| Xia H, Zhao H, Yang W Et al. MiR-195-5p represses inflammation, apoptosis, oxidative stress, and endoplasmic reticulum stress in sepsis-induced myocardial injury by targeting activating transcription factor 6 Cell biology international 2021-11-24 [PMID: 34816499] (WB, Mouse) |
WB |
Mouse |
| Li S, Zhao F, Tang Q Et al. Sarco/endoplasmic reticulum Ca2+ -ATPase 2b mediates oxidation-induced endoplasmic reticulum stress to regulate neuropathic pain British journal of pharmacology 2021-11-22 [PMID: 34811737] |
|
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| Fan Y, Wolford B, Lu H Et al. Type 2 diabetes sex-specific effects associated with E167K coding variant in TM6SF2 iScience 2021-11-01 [PMID: 34746691] (WB, Mouse) |
WB |
Mouse |
| Chunhacha P, Pinkaew D, Sinthujaroen P Et al. Fortilin inhibits p53, halts cardiomyocyte apoptosis, and protects the heart against heart failure Cell death discovery 2021-10-23 [PMID: 34689154] (WB, Mouse) |
WB |
Mouse |
| Khoi CS, Lin YW, Chen JH Et al. Selective Activation of Endoplasmic Reticulum Stress by Reactive-Oxygen-Species-Mediated Ochratoxin A-Induced Apoptosis in Tubular Epithelial Cells International journal of molecular sciences 2021-10-11 [PMID: 34681610] (WB, Human) |
WB |
Human |
| Mareninova OA, Dillon DL, Wightman CJM Et al. Rab9 Mediates Pancreatic Autophagy Switch From Canonical to Noncanonical, Aggravating Experimental Pancreatitis Cellular and molecular gastroenterology and hepatology 2021-10-02 [PMID: 34610499] |
|
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| Shaban MS, MUller C, Mayr-Buro C et al. Multi-level inhibition of coronavirus replication by chemical ER stress Nature communications 2021-09-20 [PMID: 34545074] (WB) |
WB |
|
| Zhao P, Huang P, Xu T Et Al. Fat body Ire1 regulates lipid homeostasis through the Xbp1s-FoxO axis in Drosophila iScience 2021-08-01 [PMID: 34381963] |
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| Einstein Jm, Perelis M, Chaim Ia Et Al. Inhibition of YTHDF2 triggers proteotoxic cell death in MYC-driven breast cancer Molecular cell 2021-06-29 [PMID: 34216543] (WB) |
WB |
|
| Witte F, Ruiz-Orera J, Mattioli C et al. A trans locus causes a ribosomopathy in hypertrophic hearts that affects mRNA translation in a protein length-dependent fashion Genome Biology 2021-12-01 [PMID: 34183069] (WB, Rat) |
WB |
Rat |
| Zhou Y, Ye X, Zhang C et al. Ufl1 deficiency causes kidney atrophy associated with disruption of endoplasmic reticulum homeostasis Journal of Genetics and Genomics 2021-05-01 [PMID: 34148841] |
|
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| Ricci D, Tutton S, Marrocco I et al. An interdomain helix in IRE1 alpha mediates the conformational change required for the sensor's activation The Journal of biological chemistry 2021-05-14 [PMID: 34000298] (WB, Human) |
WB |
Human |
| Baudi I, Isogawa M, Moalli F et al. Interferon signaling suppresses the unfolded protein response and induces cell death in hepatocytes accumulating hepatitis B surface antigen PLOS Pathogens 2021-05-12 [PMID: 33979382] (Block/Neutralize) |
Block/Neutralize |
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| Zhemkov V, Ditlev JA, Lee WR et al. The role of sigma 1 receptor in organization of endoplasmic reticulum signaling microdomains eLife 2021-05-11 [PMID: 33973848] (WB, Human) |
WB |
Human |
| Zhang Y, Wu N, Gan-Schreier H et al. FATP4 inactivation in cultured macrophages attenuates M1- and ER stress-induced cytokine release via a metabolic shift towards triacylglycerides The Biochemical journal 2021-04-26 [PMID: 33900381] |
|
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| Cao X, Shu Y, Chen Y et al. Mettl14-Mediated m6A Modification Facilitates Liver Regeneration by Maintaining Endoplasmic Reticulum Homeostasis Cellular and molecular gastroenterology and hepatology 2021-04-10 [PMID: 33848642] (WB, Mouse) |
WB |
Mouse |
| Rigby MJ, Lawton AJ, Kaur G et al. Endoplasmic reticulum acetyltransferases Atase1 and Atase2 differentially regulate reticulophagy, macroautophagy and cellular acetyl-CoA metabolism Communications biology 2021-04-12 [PMID: 33846551] (WB, Mouse) |
WB |
Mouse |
| Lim Y, Kim S, Kim EK Palmitate reduces starvation-induced ER stress by inhibiting ER-phagy in hypothalamic cells Molecular brain 2021-04-06 [PMID: 33823883] |
|
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| Pandey R, Bakay M, Strenkowski BP et al. JAK/STAT inhibitor therapy partially rescues the lipodystrophic autoimmune phenotype in Clec16a KO mice Scientific reports 2021-04-01 [PMID: 33795715] (WB, Mouse) |
WB |
Mouse |
| Parthibane V, Lin J, Acharya D et al. SSSPTA is essential for serine palmitoyltransferase function during development and hematopoiesis Journal of Biological Chemistry 2021-03-01 [PMID: 33662400] (WB, Mouse) |
WB |
Mouse |
| Chen N, Zhang Y, Wang M et al. Maternal Obesity Interrupts the Coordination of the Unfolded Protein Response and Heat Shock Response in the Postnatal Developing Hypothalamus of Male Offspring in Mice Molecular and cellular endocrinology 2021-02-23 [PMID: 33636254] (WB, Mouse) |
WB |
Mouse |
| Cao X, An J, Cao Y et al. EMC3 Is Essential for Retinal Organization and Neurogenesis During Mouse Retinal Development Investigative ophthalmology & visual science 2021-02-01 [PMID: 33605987] (ICC/IF, WB, Mouse) |
ICC/IF, WB |
Mouse |
| Elahe Zarini-Gakiye, Gholamhassan Vaezi, Kazem Parivar, Nima Sanadgol Age and Dose-Dependent Effects of Alpha-Lipoic Acid on Human Microtubule- Associated Protein Tau-Induced Endoplasmic Reticulum Unfolded Protein Response: Implications for Alzheimer's Disease. CNS & neurological disorders drug targets 2022-01-12 [PMID: 33573583] |
|
|
| De Nisco NJ, Casey AK, Kanchwala M et al. Manipulation of IRE1-Dependent MAPK Signaling by a Vibrio Agonist-Antagonist Effector Pair mSystems 2021-02-09 [PMID: 33563785] (WB, Mouse) |
WB |
Mouse |
| Wanschel ACBA, Guizoni DM, Lorza-Gil E, et al. The Presence of Cholesteryl Ester Transfer Protein (CETP) in Endothelial Cells Generates Vascular Oxidative Stress and Endothelial Dysfunction Biomolecules 2021-01-07 [PMID: 33430172] |
|
|
| Zhou L, Song Z, Hu J et al. ACSS3 represses prostate cancer progression through downregulating lipid droplet-associated protein PLIN3 Theranostics 2020-11-06 [PMID: 33391508] (WB, Human) |
WB |
Human |
| Kalish BT, Kim E, Finander B et al. Maternal immune activation in mice disrupts proteostasis in the fetal brain Nature neuroscience 2020-12-23 [PMID: 33361822] |
|
|
| Bontemps-Karcher A, Magneron V, Conquet L et al. Renal adaptive response to exposure to low doses of uranyl nitrate and sodium fluoride in mice Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS) 2020-12-17 [PMID: 33360916] (IHC-P, Mouse) |
IHC-P |
Mouse |
| Arioka Y, Shishido E, Kushima I et al. Chromosome 22q11.2 deletion causes PERK-dependent vulnerability in dopaminergic neurons EBioMedicine 2020-12-17 [PMID: 33341442] (WB, Human) |
WB |
Human |
| Lanzillotta C, Greco V, Valentini D et al. Proteomics Study of Peripheral Blood Mononuclear Cells in Down Syndrome Children Stem Cells 2020-11-07 [PMID: 33187268] (WB, Mouse) |
WB |
Mouse |
| O'Brien CE, Santos PT, Kulikowicz E et al. Neurologic effects of short-term treatment with a soluble epoxide hydrolase inhibitor after cardiac arrest in pediatric swine Elife 2020-10-19 [PMID: 33129262] |
|
|
| Mello A, Hsu MF, Koike S et al. Soluble epoxide hydrolase hepatic deficiency ameliorates alcohol-associated liver disease Cell Mol Gastroenterol Hepatol 2020-10-14 [PMID: 33068774] (WB, Mouse) |
WB |
Mouse |
| Xiong Z, Yuan C, Shi J et al. Restoring the epigenetically silenced PCK2 suppresses renal cell carcinoma progression and increases sensitivity to sunitinib by promoting endoplasmic reticulum stress Theranostics 2020-09-15 [PMID: 33052225] (WB, Human) |
WB |
Human |
| Chen Y, Griffiths A, Wang J et al. Inositol requiring enzyme 1 alpha (IRE1a) links palmitate-induced mTOR activation and lipotoxicity in Hepatocytes Am J Physiol Cell Physiol 2020-10-14 [PMID: 33052067] |
|
|
| Kim H, Cho Y, Kim HS et al. A system-level approach identifies HIF-2 alpha as a critical regulator of chondrosarcoma progression Nat Commun 2020-10-06 [PMID: 33024108] (WB, Rat, Human) |
WB |
Rat, Human |
| Ding R, Ou W, Chen C et al. Endoplasmic reticulum stress and oxidative stress contribute to neuronal pyroptosis caused by cerebral venous sinus thrombosis in rats: involvement of TXNIP/peroxynitrite-NLRP3 inflammasome activation Neurochem. Int. 2020-09-24 [PMID: 32980492] (WB, Rat) |
WB |
Rat |
| Zhao Q, Zhong J, Bi Y et al. Gambogenic acid induces Noxa-mediated apoptosis in colorectal cancer through ROS-dependent activation of IRE1alpha/JNK Phytomedicine 2020-01-01 [PMID: 32854039] |
|
|
| Lanzillotta C, Zuliani I, Tramutola A et al. Chronic PERK induction promotes Alzheimer-like neuropathology in Down syndrome: Insights for therapeutic intervention Prog. Neurobiol. 2020-08-11 [PMID: 32795489] (WB, Mouse, Human) |
WB |
Mouse, Human |
| Aryal YP, Lee ES, Kim TY et al. Stage-specific expression patterns of ER stress-related molecules in mice molars: Implications for tooth development Gene Expr. Patterns 2020-08-03 [PMID: 32758541] (IHC-P, Mouse) |
IHC-P |
Mouse |
| Ganesan M, Mathews S, Makarov E et al. Acetaldehyde Suppresses HBV-MHC Class I Complex Presentation on Hepatocytes Via Induction of ER Stress and Golgi Fragmentation Am. J. Physiol. Gastrointest. Liver Physiol. 2020-08-05 [PMID: 32755306] |
|
|
| Winnay JN, Solheim MH, Sakaguchi M et al. Inhibition of the PI 3-kinase pathway disrupts the unfolded protein response and reduces sensitivity to ER stress-dependent apoptosis FASEB J. 2020-08-03 [PMID: 32744782] |
|
|
| Galeone A, Adams JM, Matsuda S et al. Regulation of BMP4/Dpp retrotranslocation and signaling by deglycosylation Elife 2020-07-28 [PMID: 32720893] |
|
|
| Yue XY, Sun XF, Che K et al. Lpl-C310R mutation is associated with impaired glucose tolerance and endoplasmic reticulum stress in skeletal muscle Biochem. Biophys. Res. Commun. 2020-08-20 [PMID: 32703455] (WB, Mouse) |
WB |
Mouse |
| Cicalese S, Okuno K, Elliott KJ et al. 78 kDa Glucose-Regulated Protein Attenuates Protein Aggregation and Monocyte Adhesion Induced by Angiotensin II in Vascular Cells Int J Mol Sci 2020-07-15 [PMID: 32679678] (WB, Rat) |
WB |
Rat |
| Hotokezaka Y, Katayama I, Nakamura T ATM-associated signalling triggers the unfolded protein response and cell death in response to stress Commun Biol 2020-07-14 [PMID: 32665601] (WB, Human) |
WB |
Human |
| Murray, H C, Dieriks, B V Et al. The unfolded protein response is activated in the olfactory system in Alzheimer's disease. Acta Neuropathol Commun 2020-07-14 [PMID: 32665027] (IF/IHC, Mouse) |
IF/IHC |
Mouse |
| Zhao Y, Ma S, Cao M, Sun C G604S-HERG mutation in LQT2 leads to autophagy via the UPR-related pathway J. Biosci. 2020-07-15 [PMID: 32661217] (WB, Human) |
WB |
Human |
| Vallejo-Gracia A, Chen IP, Perrone R et al. FOXO1 promotes HIV latency by suppressing ER stress in T cells Nature Microbiology 2020-06-15 [PMID: 32541947] |
|
|
| Bae SJ, Kim JE, Choi HJ et al. ?-Linolenic Acid-Enriched Cold-Pressed Perilla Oil Suppress High-Fat Diet-Induced Hepatic Steatosis through Amelioration of the ER Stress-Mediated Autophagy Molecules 2020-06-08 [PMID: 32521713] |
|
|
| Wu XL, Deng MZ, Gao ZJ et al. Neferine alleviates memory and cognitive dysfunction in diabetic mice through modulation of the NLRP3 inflammasome pathway and alleviation of endoplasmic-reticulum stress Int. Immunopharmacol. 2020-05-08 [PMID: 32402951] (WB, Mouse) |
WB |
Mouse |
| Caroline Dafflon, Swann Gaulis, Louise Barys, Karen Kapur, Vanessa Cornacchione, Lina Schukur, Sebastian Bergling, Elisabetta Traggiai, Selina Jansky, Leon Hellmann, Barbara Schacher Engstler, Grainne Kerr, Antoine de Weck, David A. Ruddy, Ulrike Naumann, Frédéric Stauffer, Christoph Gaul, Ying Lin, Eric Billy, Andreas Weiss, Francesco Hofmann, Moriko Ito, Ralph Tiedt DOT1L inhibition is lethal for multiple myeloma due to perturbation of the endoplasmic reticulum stress pathway Oncotarget 2020-03-17 [PMID: 32215184] |
|
|
| Venkatesh P, Kumar S, Kumar N, et al. Molecular mechanisms underlying attenuation of live attenuated Japanese encephalitis virus vaccine SA14-14-2 bioRxiv 2021-01-15 (WB, Mouse, Porcine) |
WB |
Mouse, Porcine |
| Bellini L, Strub T, Habel N et al. Endoplasmic reticulum stress mediates resistance to BCL-2 inhibitor in uveal melanoma cells Cell Death Discov 2020-04-17 [PMID: 32337074] |
|
|
| Garufi A, Federici G, Gilardini Montani Ms et Al. Interplay between Endoplasmic Reticulum (ER) Stress and Autophagy Induces Mutant p53H273 Degradation Biomolecules 2020-03-03 [PMID: 32138264] (WB, Human) |
WB |
Human |
| Liu XQ, Deng YX, Dai Z et al. Sodium tanshinone IIA sulfonate protects against A alpha 1-42-induced cellular toxicity by modulating A beta-degrading enzymes in HT22 cells Int. J. Biol. Macromol. 2020-02-06 [PMID: 32035959] (WB, Human) |
WB |
Human |
| Maeyashiki C, Melhem H, Hering L et al. Activation of pH-Sensing Receptor OGR1 (GPR68) Induces ER Stress Via the IRE1 alpha/JNK Pathway in an Intestinal Epithelial Cell Model Sci Rep 2020-01-29 [PMID: 31996710] (WB, Human) |
WB |
Human |
| Han L, Bittner S, Dong D et al. Molecular changes in hepatic metabolism in ZDSD rats-A new polygenic rodent model of obesity, metabolic syndrome, and diabetes Biochim Biophys Acta Mol Basis Dis 2020-01-24 [PMID: 31987840] (WB, Rat) |
WB |
Rat |
| Younan P, Santos RI, Ramanathan P et al. Ebola virus-mediated T-lymphocyte depletion is the result of an abortive infection PLoS Pathog. 2019-10-01 [PMID: 31648236] (WB) |
WB |
|
| Li J, Lei X, Ruan Y et al. Inhibition of Mitochondrial Fission Reverses the Immunoescape of Solid Tumors via IRE1 alpha-XBP-1s-TPP2 axis Research Square 2020-08-14 (WB, Mouse, Human) |
WB |
Mouse, Human |
| Woo M, Choi HI, Park SH et al. The unc-51 like autophagy activating kinase 1-autophagy related 13 complex has distinct functions in tunicamycin-treated cells Biochem. Biophys. Res. Commun. 2020-02-05 [PMID: 32035621] |
|
|
| |
|
|
| Li C, Deng M, Gao Z et al. Effects of compound K, a metabolite of ginsenosides, on memory and cognitive dysfunction in db/db mice involves inhibition of ER stress and NLRP3 inflammasome pathway Food Funct. 2020-04-13 [PMID: 32374299] (WB) |
WB |
|
| Marcos F. Fondevila, Uxia Fernandez, Maria J. Gonzalez‐Rellan, Natalia Da Silva Lima, Xabier Buque, Agueda Gonzalez‐Rodriguez, Cristina Alonso, Marta Iruarrizaga‐Lejarreta, Teresa C. Delgado, Marta Varela‐Rey, Ana Senra, Vera Garcia‐Outeiral, Eva Novoa, Cristina Iglesias, Begoña Porteiro, Daniel Beiroa, Cintia Folgueira, Marta Tojo, Jorge L. Torres, Lourdes Hernández‐Cosido, Óscar Blanco, Juan Pablo Arab, Francisco Barrera, Diana Guallar, Miguel Fidalgo, Miguel López, Carlos Dieguez, Miguel Marcos, Maria L. Martinez‐Chantar, Marco Arrese, Carmelo Garcia‐Monzon, Jose M. Mato, Patricia Aspichueta, Ruben Nogueiras The L‐ alpha ‐Lysophosphatidylinositol/G Protein–Coupled Receptor 55 System Induces the Development of Nonalcoholic Steatosis and Steatohepatitis Hepatology (Baltimore, Md.) 2020-11-13 [PMID: 32329085] |
|
|
| Liu D, Zhang P, Zhou J et al. TNFAIP3 Interacting Protein 3 Overexpression Suppresses Nonalcoholic Steatohepatitis by Blocking TAK1 Activation Cell Metab. 2020-04-07 [PMID: 32268115] |
|
|
| Hinte F, van Anken E, Tirosh B, Brune W Repression of viral gene expression and replication by the unfolded protein response effector XBP1u Elife 2020-02-17 [PMID: 32065579] (WB, Mouse) |
WB |
Mouse |
| Yoshikawa Y, Sugimoto K, Ochiai Y, Ohashi N Intracellular proliferation of Anaplasma phagocytophilum is promoted via modulation of endoplasmic reticulum stress signaling in host cells Microbiol. Immunol. 2020-01-07 [PMID: 31909489] |
|
|
| Wang M, Law ME, Davis BJ, et al. Disulfide bond-disrupting agents activate the tumor necrosis family-related apoptosis-inducing ligand/death receptor 5 pathway Cell Death Discov 2019-12-10 [PMID: 31839995] |
|
|
| Gu R, Huang T, Xiao J et al. The IRE1 alpha Arm of UPR Regulates Muscle Cells Immune Characters by Restraining p38 MAPK Activation Front Physiol 2019-09-19 [PMID: 31607947] (IF/IHC, WB, Mouse) |
IF/IHC, WB |
Mouse |
| Velazquez KT, Enos RT, Bader JE et al. Prolonged high-fat-diet feeding promotes non-alcoholic fatty liver disease and alters gut microbiota in mice World J Hepatol 2019-08-27 [PMID: 31528245] (WB, Mouse) |
WB |
Mouse |
| Mu K, Sun Y, Zhao Y et al. Hepatic nitric oxide synthase 1 adaptor protein regulates glucose homeostasis and hepatic insulin sensitivity in obese mice depending on its PDZ binding domain EBioMedicine 2019-08-28 [PMID: 31473185] (WB, Mouse) |
WB |
Mouse |
| Ming Y, Zhu X, Tuma-Kellner S et al. iPla2v beta Deficiency Suppresses Hepatic ER UPR, Fxr, and Phospholipids in Mice Fed with MCD Diet, Resulting in Exacerbated Hepatic Bile Acids and Biliary Cell Proliferation Cells 2019-08-12 [PMID: 31409057] (WB, Mouse) |
WB |
Mouse |
| Comitato A, Schiroli D, Montanari M, Marigo V Calpain Activation Is the Major Cause of Cell Death in Photoreceptors Expressing a Rhodopsin Misfolding Mutation Mol. Neurobiol. 2019-08-10 [PMID: 31401765] |
|
|
| Xia Z, Wu S, Wei X et al. Hypoxic ER stress suppresses beta-catenin expression and promotes cooperation between the transcription factors XBP1 and HIF1 alpha for cell survival J. Biol. Chem. 2019-07-26 [PMID: 31350332] (WB, Human) |
WB |
Human |
| Shin GC, Moon SU, Kang HS et al. PRKCSH contributes to tumorigenesis by selective boosting of IRE1 signaling pathway Nat Commun 2019-07-18 [PMID: 31320625] (WB) |
WB |
|
| Han S, Mao L et al. Sec62 Suppresses Foot-and-Mouth Disease Virus Proliferation by Promotion of IRE1 alpha-RIG-I Antiviral Signaling. J Immunol 2019-07-15 [PMID: 31167774] (WB, Golden Syrian Hamster) |
WB |
Golden Syrian Hamster |
| Zheng W, Xie W, Yin D, et al. ATG5 and ATG7 induced autophagy interplays with UPR via PERK signaling Cell Commun. Signal 2019-05-06 [PMID: 31060556] (WB, Human) |
WB |
Human |
| Cui J, Xu H, Shi J et al. Carbonic anhydrase IX inhibitor S4 triggers immunogenic cell death in glioma cells via endoplasmic reticulum stress pathway Research Square 2022-06-17 (WB, Human) |
WB |
Human |
| Naughton M, Healy S, McMahon J, FitzGerald U Profile of the Unfolded Protein Response in Rat Cerebellar Cortical Development J. Comp. Neurol. 2019-05-27 [PMID: 31132146] |
|
|
| O'Brien, C E, Santos, P T Et al. Hypoxia-Ischemia and Hypothermia Independently and Interactively Affect Neuronal Pathology in Neonatal Piglets with Short-Term Recovery. Dev Neurosci 2019-05-21 [PMID: 31108487] (IF/IHC, Mouse) |
IF/IHC |
Mouse |
| Schiattarella, GG;Altamirano, F;Tong, D;French, KM;Villalobos, E;Kim, SY;Luo, X;Jiang, N;May, HI;Wang, ZV;Hill, TM;Mammen, PPA;Huang, J;Lee, DI;Hahn, VS;Sharma, K;Kass, DA;Lavandero, S;Gillette, TG;Hill, JA; Nitrosative stress drives heart failure with preserved ejection fraction Nature 2019-04-01 [PMID: 30971818] (WB, Rat) |
WB |
Rat |
| Cicalese S Exploring the Role of Smooth Muscle GRP78 in Angiotensin II-Induced Vascular Amyloid Deposition and Remodeling Thesis 2022-01-01 |
|
|
| Wiersma VI, van Ziel AM, Vazquez-Sanchez S, et al. Granulovacuolar degeneration bodies are neuron-selective lysosomal structures induced by intracellular tau pathology Acta Neuropathol. 2019-12-01 [PMID: 31456031] (IF/IHC, Mouse) |
IF/IHC |
Mouse |
| Inoue T, Yamakage H, Tanaka M et al. Oxytocin Suppresses Inflammatory Responses Induced by Lipopolysaccharide through Inhibition of the eIF-2-ATF4 Pathway in Mouse Microglia Cells. 2019-05-31 [PMID: 31159306] (Knockdown Validated, WB, Mouse) |
Knockdown Validated, WB |
Mouse |
| Li H, O'Meara M, Zhang X et al. Ameliorating Methylglyoxal-Induced Progenitor Cell Dysfunction for Tissue Repair in Diabetes Diabetes 2019-03-18 [PMID: 30885990] (WB, Mouse) |
WB |
Mouse |
| Lee MR, Bae SJ, Kim JE et al. Inhibition of endoplasmic reticulum stress in high-fat-diet-induced obese C57BL/6 mice: Efficacy of a novel extract from mulberry (Morus alba) leaves fermented with Cordyceps militaris. Lab Anim Res 2018-12-01 [PMID: 30671117] (WB, Mouse) |
WB |
Mouse |
| Yang F, Liu Y, Ren H et al. ER-stress regulates macrophage polarization through pancreatic EIF-2alpha kinase Cell. Immunol. 2018-12-21 [PMID: 30594305] (WB, Mouse) |
WB |
Mouse |
| Hong M. Regulation of Human Pancreatic B-cell Proliferation by Localization, Interaction, and Transcription of Endogenously Expressed Cell Cycle Molecules Thesis 2017-01-01 (Human) |
|
Human |
| |
|
|
| Otto AC, Gan-Schreier H, Zhu X et al. Group VIA phospholipase A2 deficiency in mice chronically fed with high-fat-diet attenuates hepatic steatosis by correcting a defect of phospholipid remodeling Biochim Biophys Acta Mol Cell Biol Lipids 2019-02-05 [PMID: 30735855] (WB, Mouse) |
WB |
Mouse |
| Wheeler MA, Jaronen M, Covacu R et al. Environmental Control of Astrocyte Pathogenic Activities in CNS Inflammation Cell 2019-01-24 [PMID: 30661753] (WB, Mouse) |
WB |
Mouse |
| Wu CH, Silvers CR, Messing EM, Lee YF. Bladder cancer extracellular vesicles drive tumorigenesis by inducing the unfolded protein response in endoplasmic reticulum of non-malignant cells. J. Biol. Chem. 2018-12-28 [PMID: 30593508] (WB, Human) |
WB |
Human |
| Sun MS, Zhang J, Jiang LQ et al. TMED2 Potentiates Cellular IFN Responses to DNA Viruses by Reinforcing MITA Dimerization and Facilitating Its Trafficking Cell Rep 2018-12-11 [PMID: 30540941] (Human) |
|
Human |
| Aggarwal S, Ahmad I, Lam A et al. Heme scavenging reduces pulmonary endoplasmic reticulum stress, fibrosis, and emphysema. JCI Insight. 2018-11-02 [PMID: 30385726] (ELISA, Mouse) |
ELISA |
Mouse |
| Pramanik Jhuma, Chen Xi, Kar Gozde et al. Genome-wide analyses reveal the IRE1a-XBP1 pathway promotes T helper cell differentiation by resolving secretory stress and accelerating proliferation. Neurochemistry International 2018-10-24 [PMID: 30355343] (WB, Mouse) |
WB |
Mouse |
| Chang TK, Lawrence DA, Lu M1 et al. Coordination between Two Branches of the Unfolded Protein Response Determines Apoptotic Cell Fate. Mol?Cell 2018-08-16 [PMID: 30118681] (WB) |
WB |
|
| Cho YM, Kim DH, Lee KH et al. The IRE1a-XBP1s pathway promotes insulin-stimulated glucose uptake in adipocytes by increasing PPAR gamma activity Exp. Mol. Med. 2018-08-15 [PMID: 30111834] (WB, Mouse) |
WB |
Mouse |
| Ricci D, Tutton S, Marrocco I et al. The connector between the kinase and RNase domains of IRE1alpha transmits the conformational change that underlies ER stress-induced activation. bioRxiv 2020-01-01 (WB, Human) |
WB |
Human |
| Fussi Natascha, Hollerhage Matthias, Chakroun Tasnim et al. Exosomal secretion of a-synuclein as protective mechanism after upstream blockage of macroautophagy. Cell Death & Disease 2018-01-01 [PMID: 29988147] (WB, Human) |
WB |
Human |
| Yang F, Wang S, Liu Y et al. IRE1a aggravates ischemia reperfusion injury of fatty liver by regulating phenotypic transformation of kupffer cells. Free Radic. Biol. Med. 2018-06-30 [PMID: 29969718] (WB, ICC/IF, Mouse) |
WB, ICC/IF |
Mouse |
| Zhang L, Cheng X, Xu S et al. Curcumin induces endoplasmic reticulum stress-associated apoptosis in human papillary thyroid carcinoma BCPAP cells via disruption of intracellular calcium homeostasis Medicine (Baltimore) 2018-06-01 [PMID: 29901626] (WB, Human) |
WB |
Human |
| Chatterjee B, Fatima F, Seth S, Sinha Roy S Moderate Elevation of Homocysteine Induces Endothelial Dysfunction through Adaptive UPR Activation and Metabolic Rewiring preprints.org 2023-09-05 (WB, Human) |
WB |
Human |
| Rubio-Patino C, Bossowski JP, De Donatis GM. Low-Protein Diet Induces IRE1A-Dependent Anticancer Immunosurveillance. Cell metabolism. 1905-07-10 [PMID: 29551590] |
|
|
| Niu Xiaowei, Zhang Jingjing, Ling Chun et al. Polysaccharide from Angelica sinensis protects H9c2 cells against oxidative injury and endoplasmic reticulum stress by activating the ATF6 pathway. The Journal of International Medical Research 2018-01-01 [PMID: 29517941] (WB, Human) |
WB |
Human |
| Xiang Q, Li N, Zhang Y et al. Adhesion GPCR GPR116 Alleviates Acetaminophen-Induced Liver Injury by Inhibiting ER Stress SSRN 2023-04-18 |
|
|
| Najarro G, Brackett K, Woosley H et al. BiP/GRP78 is a pro-viral factor for diverse dsDNA viruses that promotes the survival and proliferation of cells upon KSHV infection bioRxiv 2023-10-01 (Western Blot, Human) |
Western Blot |
Human |
| Pommier A, Anaparthy N et al. Unresolved endoplasmic reticulum stress engenders immune-resistant, latent pancreatic cancer metastases. Science 2018-06-15 [PMID: 29773669] (IF/IHC, FLOW, Mouse) |
IF/IHC, FLOW |
Mouse |
| Steiger D, Yokota T, Li J et al. The serine/threonine-protein kinase/endoribonuclease IRE1alpha protects the heart against pressure overload-induced heart failure. J Biol Chem 2018-06-22 [PMID: 29769316] (Mouse) |
|
Mouse |
| Choi S, Snider JM, Olakkengil N et al. Myristate-induced endoplasmic reticulum stress requires ceramide synthases 5/6 and generation of C14-ceramide in intestinal epithelial cells FASEB J. 2018-05-16 [PMID: 29768040] |
|
|
| Wang B, Su C, Liu T et al. The Neuroprotection of Low-Dose Morphine in Cellular and Animal Models of Parkinson’s Disease Through Ameliorating Endoplasmic Reticulum (ER) Stress and Activating Autophagy. Front. Mol. Neurosci. 2018-04-20 [PMID: 29731707] (WB, Human) |
WB |
Human |
| Kazama H, Hiramoto M, Miyahara K et al. Designing an effective drug combination for ER stress loading in cancer therapy using a real-time monitoring system Biochem. Biophys. Res. Commun. 2018-06-18 [PMID: 29729272] (WB, Human) |
WB |
Human |
| Bi X, Zhang G, Wang X et al. ER Chaperone GRP78 Protects Heart from Ischemia/Reperfusion Injury Through Akt Activation. Circ. Res. 2018-04-18 [PMID: 29669712] (Rat) |
|
Rat |
| Xiaoding Wang, Xukun Bi, Guangyu Zhang, Yingfeng Deng, Xiang Luo, Lin Xu, Philipp E. Scherer, Anwarul Ferdous, Guosheng Fu, Thomas G. Gillette, Amy S. Lee, Xuejun Jiang, Zhao V. Wang Glucose-regulated protein 78 is essential for cardiac myocyte survival Cell Death and Differentiation 2018-04-17 [PMID: 29666470] |
|
|
| Li C, Fan Q, Quan H et al. The three branches of the unfolded protein response exhibit differential significance in breast cancer growth and stemness. Exp. Cell Res. 2018-03-27 [PMID: 29601799] (Human) |
|
Human |
| Crider A, Nelson T, Davis T, Fagan K. Estrogen Receptor b Agonist Attenuates Endoplasmic Reticulum Stress-Induced Changes in Social Behavior and Brain Connectivity in Mice. Mol. Neurobiol. 2018-02-12 [PMID: 29430617] (Mouse) |
|
Mouse |
| Liu X, Guo GL, Kong B, Hilburn DB. Farnesoid X Receptor Signaling Activates the Hepatic X-box Binding Protein 1 Pathway in vitro and in Mice. Hepatology. 2018-01-29 [PMID: 29377207] (Mouse) |
|
Mouse |
| Amer SM, Bhopale KK, Kakumanu RD et al. Hepatic alcohol dehydrogenase deficiency induces pancreatic injury in chronic ethanol feeding model of deer mice. Exp Mol Pathol 2018-02-01 [PMID: 29337245] |
|
|
| Perteiro B, Fondevila M, Buque X et al. Pharmacological stimulation of p53 with low-dose doxorubicin ameliorates diet-induced nonalcoholic steatosis and steatohepatitis Mol Metab. 2017-12-15 [PMID: 29290620] (WB, Human, Rat) |
WB |
Human, Rat |
| Ma L, Liu Y, Landry NK et al. Point mutation in D8C domain of Tamm-Horsfall protein/uromodulin in transgenic mice causes progressive renal damage and hyperuricemia PLoS ONE 2017-11-16 [PMID: 29145399] (ICC/IF) |
ICC/IF |
|
| Yasui Tadashi, Gomi Hiroshi, Kitahara Taishi, Tsukise Azuma Ultrastructure and immunohistochemical characterization of proteins concerned with the secretory machinery in goat ceruminous glands. European Journal of Histochemistry : Ejh 2017-08-08 [PMID: 29046053] (IHC-P, Goat) |
IHC-P |
Goat |
| Ji C, Yang B, Huang SY et al. Salubrinal protects human skin fibroblasts against UVB-induced cell death by blocking endoplasmic reticulum (ER) stress and regulating calcium homeostasis. Biochem. Biophys. Res. Commun. 2017-12-02 [PMID: 28988108] (Human) |
|
Human |
| Sun J, Ren D. IER3IP1 deficiency leads to increased B-cell death and decreased B-cell proliferation Oncotarget 2017-08-22 [PMID: 28915629] (WB, Human) |
WB |
Human |
| Plaas M, Seppa K, Reimets R et al. Wfs1- deficient rats develop primary symptoms of Wolfram syndrome: insulin-dependent diabetes, optic nerve atrophy and medullary degeneration Sci Rep 2017-08-31 [PMID: 28860598] (IF/IHC, Rat) |
IF/IHC |
Rat |
| Zhang X, Wu WK, Xu W et al. CXC Motif Chemokine 10 Impairs Autophagy and Autolysosome Formation in Non-alcoholic Steatohepatitis Theranostics. [PMID: 28824718] (WB, Human) |
WB |
Human |
| Zhang XH, Wang XY, Zhou ZW et al. The combination of digoxin and GSK2606414 exerts synergistic anticancer activity against leukemia in vitro and in vivo Biofactors 2017-08-17 [PMID: 28817203] (Human) |
|
Human |
| Rius B, Duran-Guell M, Flores-Costa R et al. The specialized pro-resolving lipid mediator maresin 1 protects hepatocytes from lipotoxic and hypoxia-induced endoplasmic reticulum stress FASEB J. 2017-08-02 [PMID: 28768719] (Mouse) |
|
Mouse |
| Guo H, Wang Y, Zhang X et al. Astragaloside IV protects against podocyte injury via SERCA2-dependent ER stress reduction and AMPKalpha-regulated autophagy induction in streptozotocin-induced diabetic nephropathy Sci Rep 2017-07-31 [PMID: 28761152] (WB, Mouse) |
WB |
Mouse |
| Nakazawa H, Ikeda K, Shinozaki S et al. Burn-induced muscle metabolic derangements and mitochondrial dysfunction are associated with activation of HIF-1alpha and mTORC1: Role of protein farnesylation Sci Rep 2017-07-26 [PMID: 28747716] (WB, Mouse) |
WB |
Mouse |
| Okumura N, Kitahara M, Okuda H et al. Sustained Activation of the Unfolded Protein Response Induces Cell Death in Fuchs' Endothelial Corneal Dystrophy Invest. Ophthalmol. Vis. Sci. 2017-07-01 [PMID: 28727885] (WB, Human) |
WB |
Human |
| Alam S, Abdullah CS, Aishwarya R et al. Sigmar1 regulates endoplasmic reticulum stress-induced C/EBP-homologous protein expression in cardiomyocytes Biosci. Rep. 2017-08-31 [PMID: 28667101] (WB, Rat) |
WB |
Rat |
| Tsai TH, Chen E, Li L et al. The Constitutive Lipid Droplet Protein Plin2 Regulates Autophagy in Liver. Autophagy 2017-05-26 [PMID: 28548876] (WB, Mouse) |
WB |
Mouse |
| Shan B, Wang X, Wu Y et al. The metabolic ER stress sensor IRE1alpha suppresses alternative activation of macrophages and impairs energy expenditure in obesity. Nat. Immunol. 2017-05-01 [PMID: 28346409] (WB, IF/IHC, Mouse) |
WB, IF/IHC |
Mouse |
| Duran-Aniotz C, Cornejo VH, Espinoza S et al. IRE1 signaling exacerbates Alzheimer's disease pathogenesis. Acta Neuropathol. 2017-03-24 [PMID: 28341998] (Human) |
|
Human |
| Marcassa E, Raimondi M, Anwar T et al. Calpain mobilizes Atg9/Bif-1 vesicles from Golgi stacks upon autophagy induction by thapsigargin. Biol Open. 2017-03-16 [PMID: 28302665] (WB, Human) |
WB |
Human |
| liu x, Huang S, Wan C et al. Melatonin Attenuates Scopolamine-induced Cognitive Dysfunction through SIRT1/IRE1?/XBP1 pathway Research Square 2023-04-20 (Immunohistochemistry, Western Blot, Mouse) |
Immunohistochemistry, Western Blot |
Mouse |
| Okumura N, Hashimoto K, Kitahara M et al. Activation of TGF-alpha signaling induces cell death via the unfolded protein response in Fuchs endothelial corneal dystrophy Sci Rep 2017-07-28 [PMID: 28754918] (WB, Human) |
WB |
Human |
| Mahmassani ZS, Son K, Pincu Y et al. a7b1 Integrin regulation of gene transcription in skeletal muscle following an acute bout of eccentric exercise. Am. J. Physiol., Cell Physiol. 2017-03-08 [PMID: 28274919] (WB, Mouse) |
WB |
Mouse |
| Teng RJ, Jing X, Michalkiewicz T et al. Attenuation of endoplasmic reticulum stress by caffeine ameliorates hyperoxia-induced lung injury. Am. J. Physiol. Lung Cell Mol. Physiol. 2017-05-01 [PMID: 28213471] (Rat) |
|
Rat |
| Liu J, Wang Y, Song L et al. A critical role of DDRGK1 in endoplasmic reticulum homoeostasis via regulation of IRE1a stability Nat Commun 2017-01-27 [PMID: 28128204] (WB, Human) |
WB |
Human |
| Guo H, Cao A, Chu S et al. Astragaloside IV Attenuates Podocyte Apoptosis Mediated by Endoplasmic Reticulum Stress through Upregulating Sarco/Endoplasmic Reticulum Ca2+-ATPase 2 Expression in Diabetic Nephropathy. Front. Pharmacol. 2016-12-21 [PMID: 28066247] (WB, Mouse) |
WB |
Mouse |
| Nathalie Launay, Montserrat Ruiz, Laia Grau, Francisco J. Ortega, Ekaterina V. Ilieva, Juan José Martínez, Elena Galea, Isidre Ferrer, Erwin Knecht, Aurora Pujol, Stéphane Fourcade Tauroursodeoxycholic bile acid arrests axonal degeneration by inhibiting the unfolded protein response in X-linked adrenoleukodystrophy Acta Neuropathologica 2016-12-21 [PMID: 28004277] |
|
|
| Enos R T, Velazquez K T et al. A Low Dose of Dietary Quercetin Fails to Protect against the Development of an Obese Phenotype in Mice. PLoS One 2016-12-14 [PMID: 27959936] (WB, Mouse) |
WB |
Mouse |
| Mukhopadhyay C, Triplett A et al. Casitas B-cell lymphoma (Cbl) proteins protect mammary epithelial cells from proteotoxicity of active c-Src accumulation. Proc Natl Acad Sci U S A 2016-12-20 [PMID: 27930322] (WB, Mouse) |
WB |
Mouse |
| Tripathi M, Zhang CW, Singh BK et al. Hyperhomocysteinemia causes ER stress and impaired autophagy that is reversed by Vitamin B supplementation. Cell Death Dis. 2016-12-08 [PMID: 27929536] |
|
|
| Takatori O, Usui S, Okajima M et al. Sodium 4-Phenylbutyrate Attenuates Myocardial Reperfusion Injury by Reducing the Unfolded Protein Response. J. Cardiovasc. Pharmacol. Ther. 2016-12-01 [PMID: 27909014] (WB, Mouse) |
WB |
Mouse |
| Liu X, Zhao J, Liu Q et al. MicroRNA-124 promotes hepatic triglyceride accumulation through targeting tribbles homolog 3. Sci Rep. 2016-11-15 [PMID: 27845424] (WB, Human) |
WB |
Human |
| Wiersma VI, van Hecke W, Scheper W et al. Activation of the unfolded protein response and granulovacuolar degeneration are not common features of human prion pathology. Acta Neuropathol Commun 2016-10-28 [PMID: 27793194] |
|
|
| Imbernon Piedra M. Efectos centrales de MCH sobre el metabolismo periferico. Thesis 2016-06-01 (IF/IHC, WB) |
IF/IHC, WB |
|
| Zanotto-Filho A, Masamsetti VP, Loranc E et al. Alkylating agent induced NRF2 blocks endoplasmic reticulum stress-mediated apoptosis via control of glutathione pools and protein thiol homeostasis Mol. Cancer Ther. 2016-09-16 [PMID: 27638861] (WB, Human) |
WB |
Human |
| Oka T, Kurozumi K, Shimazu Y et al. A super gene expression system enhances the anti-glioma effects of adenovirus-mediated REIC/Dkk-3 gene therapy Sci Rep 2016-09-14 [PMID: 27625116] (WB, Mouse) |
WB |
Mouse |
| Xiao Y, Xia T, Yu J et al. Knockout of inositol-requiring enzyme 1alpha in pro-opiomelanocortin neurons decreases fat mass via increasing energy expenditure. Open Biol. 2016-08-01 [PMID: 27558934] (ICC/IF, WB, Mouse) |
ICC/IF, WB |
Mouse |
| Kim J, Youn I, Seo E, Kim C Protective effects of Allium macrostemon extract on adipose tissue and liver dysregulation induced by high-fat diet and bisphenol A in C57BL/6 mice Research Square 2023-06-27 (WB, Mouse) |
WB |
Mouse |
| Bastola P, Neums L, Schoenen FJ, Chien J VCP inhibitors induce endoplasmic reticulum stress, cause cell cycle arrest, trigger caspase-mediated cell death and synergistically kill ovarian cancer cells in combination with Salubrinal. Mol Oncol 2016-09-28 [PMID: 27729194] (WB) |
WB |
|
| Nguyen KNY Examination of Endoplasmic Reticulum Stress, the Unfolded Protein Response and Autophagy in Iron Overload-Induced Insulin Resistance Thesis 2023-01-01 (WB, Rat) |
WB |
Rat |
| M. Catarina Silva, Chialin Cheng, Waltraud Mair, Sandra Almeida, Helen Fong, M. Helal U. Biswas, Zhijun Zhang, Yadong Huang, Sally Temple, Giovanni Coppola, Daniel H. Geschwind, Anna Karydas, Bruce L. Miller, Kenneth S. Kosik, Fen-Biao Gao, Judith A. Steen, Stephen J. Haggarty Human iPSC-Derived Neuronal Model of Tau-A152T Frontotemporal Dementia Reveals Tau-Mediated Mechanisms of Neuronal Vulnerability Stem Cell Reports 2016-09-01 [PMID: 27594585] |
|
|
| Kishi H, Sato M, Shibata Y et al. Role of chemokine C-C motif ligand-1 in acute and chronic pulmonary inflammations. Springerplus. 2016-08-18 [PMID: 27536524] (IF/IHC, Mouse) |
IF/IHC |
Mouse |
| Kwanten W J, Vandewynckel Y P et al. Hepatocellular autophagy modulates the unfolded protein response and fasting-induced steatosis in mice. Am J Physiol Gastrointest Liver Physiol 2016-01-10 [PMID: 27514483] (IF/IHC, Mouse) |
IF/IHC |
Mouse |
| Takatani T, Shirakawa J, Roe MW et al. IRS1 deficiency protects Beta -cells against ER stress-induced apoptosis by modulating sXBP-1 stability and protein translation Sci Rep 2016-07-05 [PMID: 27378176] (WB) |
WB |
|
| Jialin G, Yao Z, Cui Y et al. Spontaneous nonalcoholic fatty liver disease and ER stress in Sidt2 deficiency mice. Biochem. Biophys. Res. Commun. 2016-05-24 [PMID: 27233614] (WB, Mouse) |
WB |
Mouse |
| Cao A, Wang L, Chen X et al. Ursodeoxycholic acid ameliorated diabetic nephropathy by attenuating hyperglycemia-mediated oxidative stress. Biol. Pharm. Bull. 2016-05-18 [PMID: 27193377] (WB, Mouse) |
WB |
Mouse |
| Mandal A, Mandal S, Park MH. Global quantitative proteomics reveal up-regulation of endoplasmic reticulum stress response proteins upon depletion of eIF5A in HeLa cells. Nature Publishing Group. 2016-05-16 [PMID: 27180817] (WB, Mammal) |
WB |
Mammal |
| Ning B, Deng M, Zhang Q et al. beta-Asarone Inhibits IRE1/XBP1 Endoplasmic Reticulum Stress Pathway in 6-OHDA-Induced Parkinsonian Rats. Neurochem Res. [PMID: 27097550] (WB, Rat) |
WB |
Rat |
| Merlot AM, Shafie NH, Yu Y et al. Mechanism of the induction of endoplasmic reticulum stress by the anti-cancer agent, di-2-pyridylketone 4,4-dimethyl-3-thiosemicarbazone (Dp44mT): Activation of PERK/eIF2alpha, IRE1alpha, ATF6 and calmodulin kinase. Biochem Pharmacol. [PMID: 27059255] (WB, Human) |
WB |
Human |
| Park MJ, Oh KS, Nho JH et al. Asymmetric dimethylarginine (ADMA) treatment induces apoptosis in cultured rat mesangial cells via endoplasmic reticulum stress activation. Cell Biol Int. [PMID: 26992443] (WB, Rat) |
WB |
Rat |
| Soeda J, Mouralidarane A, Cordero P et al. Maternal obesity alters endoplasmic reticulum homeostasis in offspring pancreas. J Physiol Biochem. [PMID: 26979740] (WB, Human) |
WB |
Human |
| Kang K, Tarchick MJ, Yu X et al. Carnosic acid slows photoreceptor degeneration in the Pde6b(rd10) mouse model of retinitis pigmentosa. Sci Rep. [PMID: 26961159] (WB, Mouse) |
WB |
Mouse |
| Aumailley L, Warren A, Garand C et al. Vitamin C modulates the metabolic and cytokine profiles, alleviates hepatic endoplasmic reticulum stress, and increases the life span of Gulo-/- mice. Aging (Albany NY) 2016-03-01 [PMID: 26922388] (WB) |
WB |
|
| Hong MN, Nam KY, Kim KK et al. The small molecule '1-(4-biphenylylcarbonyl)-4-(5-bromo-2-methoxybenzyl) piperazine oxalate' and its derivatives regulate global protein synthesis by inactivating eukaryotic translation initiation factor 2-alpha. Cell Stress Chaperones 2016-05-01 [PMID: 26873011] (WB, Rat) |
WB |
Rat |
| Hong K, Xu G, Grayson TB, Shalev A Cytokines Regulate beta-Cell Thioredoxin-interacting Protein (TXNIP) via Distinct Mechanisms and Pathways. J Biol Chem 2016-04-15 [PMID: 26858253] |
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| Li Y, Zalzala M, Jadhav K et al. Carboxylesterase 2 prevents liver steatosis by modulating lipolysis, ER stress and lipogenesis and is regulated by HNF4alpha. Hepatology. 2016-01-25 [PMID: 26806650] |
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| Stijnen P, Brouwers B, Dirkx E et al. Endoplasmic reticulum-associated degradation of the mouse PC1/3-N222D hypomorph and human PCSK1 mutations contributes to obesity. Int J Obes (Lond) 2016-06-01 [PMID: 26786350] |
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| Tao K, Wang B, Feng D et al. Salidroside Protects Against 6-Hydroxydopamine-Induced Cytotoxicity by Attenuating ER Stress. Neurosci Bull. 2016-02-01 [PMID: 26762342] (WB, ICC/IF, Mouse) |
WB, ICC/IF |
Mouse |
Tampakakis E, Tabit CE, Holbrook M et al. Intravenous Lipid Infusion Induces Endoplasmic Reticulum Stress in Endothelial Cells and Blood Mononuclear Cells of Healthy Adults. J Am Heart Assoc. 2016-01-12 [PMID: 26755554] (ICC/IF, Human)
Details:
p-IRE1 (Ser724) antibody used for Immunofluorescence staining on human PMBCs that were untreated or treated with intralipid for 5 hours (Figure 4C and D). |
ICC/IF |
Human |
| Lopez-Gonzalez I, Perez-Mediavilla A, Zamarbide M et al. Limited Unfolded Protein Response and Inflammation in Neuroserpinopathy. J Neuropathol Exp Neurol. [PMID: 26733586] (WB, Human) |
WB |
Human |
| Vannuvel K, Van Steenbrugge M, Demazy C et al. Effects of a Sublethal and Transient Stress of the Endoplasmic Reticulum on the Mitochondrial Population. J. Cell. Physiol. 2015-12-17 [PMID: 26680008] (WB, Human) |
WB |
Human |
| El-Khattouti A, Selimovic D, Hannig M et al. Imiquimod-induced apoptosis of melanoma cells is mediated by ER stress-dependent Noxa induction and enhanced by NF-kB inhibition. J Cell Mol Med. [PMID: 26578344] (WB, Human) |
WB |
Human |
| Kim Ok-Kyung, Nam Da-Eun, Jun Woojin, Lee Jeongmin. Cudrania tricuspidata water extract improved obesity-induced hepatic insulin resistance in db/db mice by suppressing ER stress and inflammation. Food Nutr Res 2015-01-01 [PMID: 26507490] (WB, Mouse) |
WB |
Mouse |
| Jensen BK, Monnerie H, Mannell MV et al. Altered Oligodendrocyte Maturation and Myelin Maintenance: The Role of Antiretrovirals in HIV-Associated Neurocognitive Disorders. J Neuropathol Exp Neurol. [PMID: 26469251] (WB, Mouse) |
WB |
Mouse |
| Aumailley L, Garand C, Dubois MJ et al. Metabolic and Phenotypic Differences between Mice Producing a Werner Syndrome Helicase Mutant Protein and Wrn Null Mice. PLoS One 2015-01-01 [PMID: 26447695] (ICC/IF, Mouse) |
ICC/IF |
Mouse |
| Concha NO, Smallwood A, Bonnette W et al. Long-Range Inhibitor-Induced Conformational Regulation of Human IRE1alpha Endoribonuclease Activity. Mol Pharmacol. [PMID: 26438213] (WB, Human) |
WB |
Human |
| Gao X, van der Veen JN1, Vance JE et al. Lack of phosphatidylethanolamine N-methyltransferase alters hepatic phospholipid composition and induces endoplasmic reticulum stress. Biochim Biophys Acta. [PMID: 26391255] (WB, Rat, Mouse) |
WB |
Rat, Mouse |
| Cai X, Chen L, McGinnis JF. Correlation of ER stress and retinal degeneration in tubby mice. Exp. Eye Res. 2015-09-08 [PMID: 26325328] (WB, Mouse) |
WB |
Mouse |
| Wang Y, Yamada E, Zong H, Pessin JE. Fyn Activation of mTORC1 Stimulate the IRE1a-JNK Pathway Leading to Cell Death. J. Biol. Chem. 2015-08-25 [PMID: 26306048] (WB, Mouse, Human) |
WB |
Mouse, Human |
| Naughton MC, McMahon JM, FitzGerald U. Differential activation of ER stress pathways in myelinating cerebellar tracts. Int. J. Dev. Neurosci. 2015-08-20 [PMID: 26297908] (IHC-P, Rat) |
IHC-P |
Rat |
| Wang Y, Kuramitsu Y, Baron B et al. PERK/CHOP contributes to the CGK733-induced vesicular calcium sequestration which is accompanied by non-apoptotic cell death. Oncotarget 2015-09-22 [PMID: 26259235] |
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| Ma H, Butler MR, Thapa A et al. cGMP/Protein Kinase G Signaling Suppresses Inositol 1,4,5-Trisphosphate Receptor Phosphorylation and Promotes Endoplasmic Reticulum Stress in Photoreceptors of Cyclic Nucleotide-gated Channel-deficient Mice. J Biol Chem. [PMID: 26124274] |
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Quan X, Wang J, Liang C et al. Melatonin inhibits tunicamycin-induced endoplasmic reticulum stress and insulin resistance in skeletal muscle cells Biochem. Biophys. Res. Commun. 2015-06-12 [PMID: 26079879] (WB, Mouse)
Details:
IRE1 alpha antibody was used for WB on the lysates of C2C12 cells ( a primary line of murine myoblasts) that were serum-starved and treated with 0.5 ug/mL tunicamycin or without in the presence or absence of 100 nM melatonin or 10 uM Luzindole for 16 hours (Fig. 3). |
WB |
Mouse |
| Bi X, He X, Xu M et al. Acetylcholine ameliorates endoplasmic reticulum stress in endothelial cells after hypoxia/reoxygenation via M3 AChR-AMPK signaling Cell Cycle. 2015-06-11 [PMID: 26066647] (WB, Human) |
WB |
Human |
| Hrincius ER, Liedmann S, Finkelstein D et al. Acute Lung Injury Results from Innate Sensing of Viruses by an ER Stress Pathway Cell Rep. 2015-06-16 [PMID: 26051937] (WB, Human) |
WB |
Human |
| Liang L, Chen J, Zhan L et al. Endoplasmic reticulum stress impairs insulin receptor signaling in the brains of obese rats. PLoS One. [PMID: 25978724] (WB, Rat) |
WB |
Rat |
| Borsa M, Ferreira PL, Petry A et al. HIV infection and antiretroviral therapy lead to unfolded protein response activation. Virol J. [PMID: 25976933] (WB, Human) |
WB |
Human |
Won Suk Jahng J, Turdi S, Kovacevic V et al. Pressure overload-induced cardiac dysfunction in aged male adiponectin knockout mice is associated with autophagy deficiency. Endocrinology. 2015-05-11 [PMID: 25961840] (WB, Mouse)
Details:
IRE1 alpha [p Ser724] antibody used for WB analysis of mouse left ventricular (LV) heart tissue after 3 weeks of pressure overload (PO) induction (Fig 6). |
WB |
Mouse |
| Shen Y, Yang J, Zhao J et al. The switch from ER stress-induced apoptosis to autophagy via ROS-mediated JNK/p62 signals: A survival mechanism in methotrexate-resistant choriocarcinoma cells Exp. Cell Res. 2015-04-24 [PMID: 25912909] (WB, Human) |
WB |
Human |
| Hotokezaka Y, Katayama I, van Leyen K, Nakamura, T. GSK-3beta-dependent downregulation of gamma-taxilin and alphaNAC merge to regulate ER stress responses Cell Death Dis 2015-04-17 [PMID: 25880086] (WB, Mouse) |
WB |
Mouse |
| Qiu B, Ackerman D, Sanchez DJ et al. HIF-2alpha dependent lipid storage promotes endoplasmic reticulum homeostasis in clear cell renal cell carcinoma Cancer Discov 2015-03-31 [PMID: 25829424] (WB, Human) |
WB |
Human |
| Narsale AA, Enos RT, Puppa MJ et al. Liver Inflammation and Metabolic Signaling in ApcMin/+ Mice: The Role of Cachexia Progression. PLoS ONE. 2015-03-20 [PMID: 25789991] (WB, Mouse) |
WB |
Mouse |
| Jeon YJ, Khelifa S, Ratnikov B et al. Regulation of Glutamine Carrier Proteins by RNF5 Determines Breast Cancer Response to ER Stress-Inducing Chemotherapies. Cancer Cell. 2015-03-09 [PMID: 25759021] (WB, Human) |
WB |
Human |
| Iwasaki N, Sugiyama Y, Miyazaki S et al. An ATF4-Signal-Modulating Machine Other Than GADD34 Acts in ATF4-to-CHOP Signaling to Block CHOP Expression in ER-Stress-Related Autophagy J. Cell. Biochem. 2015-03-03 [PMID: 25737469] (WB, Human) |
WB |
Human |
| Wu H, Wei L, Fan F et al. Integration of Hippo signalling and the unfolded protein response to restrain liver overgrowth and tumorigenesis. Nat Commun. [PMID: 25695629] |
|
|
| Walter F, Schmid J, Dussmann H et al. Imaging of single cell responses to ER stress indicates that the relative dynamics of IRE1/XBP1 and PERK/ATF4 signalling rather than a switch between signalling branches determine cell survival Cell Death Differ. 2015-01-30 [PMID: 25633195] (WB, Human) |
WB |
Human |
| Clarke JR, Lyra E Silva NM, Figueiredo CP et al. Alzheimer-associated Abeta oligomers impact the central nervous system to induce peripheral metabolic deregulation. EMBO Mol Med 2015-02-01 [PMID: 25617315] (WB) |
WB |
|
| Jaishy B, Zhang Q, Chung HS et al. Lipid-induced NOX2 activation inhibits autophagic flux by impairing lysosomal enzyme activity. J Lipid Res. [PMID: 25529920] (WB, Mouse) |
WB |
Mouse |
| Kim DI, Park MJ, Choi JH et al. Hyperglycemia-induced GLP-1R downregulation causes RPE cell apoptosis. Int J Biochem Cell Biol. [PMID: 25483438] (WB, Human) |
WB |
Human |
Ishimura S, Furuhashi M, Mita T et al. Reduction of endoplasmic reticulum stress inhibits neointima formation after vascular injury Sci Rep et al. 2014-11-06 [PMID: 25373918] (IHC-P, WB, Mouse)
Details:
IRE1 alpha [p Ser724] antibody used for WB and IHC-P on experiments involving mouse's wire injury model (endoluminal injury of the left femoral artery). IHC-P - femoral arteries fixed in 10% formalin, sectioned at ten locations at 100-um intervals, On 4C primary antibody incubation (Figure 1D). WB - Antibody used as ER stress marker in Xbp1-knockdown human coronary artery smooth muscle cells (CASMC) treated or not with PDGF-BB, 4-PBA and TUDCA under various in-vitro treatment conditions (Figure 2H, Figure 4A, Figure 4B, Figure S4, ) |
IHC-P, WB |
Mouse |
| Yang Y, Sun M, Shan Y et al. Endoplasmic Reticulum Stress-Mediated Apoptotic Pathway Is Involved in Corpus Luteum Regression in Rats. Reprod Sci. 2014-10-20 [PMID: 25332219] (IHC-P, Mouse) |
IHC-P |
Mouse |
| Wu Y, Yang M, Fan J et al. Deficiency of osteoblastic Arl6ip5 impaired osteoblast differentiation and enhanced osteoclastogenesis via disturbance of ER calcium homeostasis and induction of ER stress-mediated apoptosis. Cell Death Dis. 2014-10-17 [PMID: 25321471] (WB, Mouse) |
WB |
Mouse |
| Park KS, Bayles I, Szlachta-McGinn A et al. Transcription Elongation Factor ELL2 Drives Ig Secretory-Specific mRNA Production and the Unfolded Protein Response. J Immunol. 2014-09-19 [PMID: 25238757] |
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|
| Zhu X, Zhang J, Sun H et al. Ubiquitination of inositol-requiring enzyme 1 (IRE1) by the E3 ligase CHIP mediates the IRE1/TRAF2/JNK pathway. J Biol Chem. 2014-09-15 [PMID: 25225294] |
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|
| Li E, Xu Z, Liu F et al. Continual exposure to cigarette smoke extracts induces tumor-like transformation of human nontumor bronchial epithelial cells in a microfluidic chip. J Thorac Oncol. [PMID: 25157762] (WB, Human) |
WB |
Human |
| Kurahashi T, Kwon M, Homma T et al. Reductive detoxification of acrolein as a potential role for aldehyde reductase (AKR1A) in mammals. Biochem. Biophys. Res. Commun. 2014-08-21 [PMID: 25152401] (WB, Mouse) |
WB |
Mouse |
Kohler C, Dinekov M, Gotz J. Granulovacuolar degeneration and unfolded protein response in mouse models of tauopathy and Ab amyloidosis. Neurobiol. Dis. 2014-07-27 [PMID: 25073087] (IHC-P, Mouse)
Details:
IRE1 alpha (phospho-Ser 724) antibody used for IHC-P in transgenic pR5 mice (which express the longest human tau isoform, htau40, together with the pathogenic mutation P301L). Brains fixed in 4%PFA, antigen retrieval performed by boiling the sections 3 times for 5 min in citrate buffer pH 6.0 using microwave, primary dilution used - 1:3500, detection by avidin-biotin peroxidase - DAB/0.6% ammonium nickel sulfate - deep blue-gray reaction product (Fig 1d). |
IHC-P |
Mouse |
Negroni A, Prete E, Vitali R et al. Endoplasmic reticulum stress and unfolded protein response are involved in paediatric inflammatory bowel disease Dig Liver Dis. 2014-06-19 [PMID: 24953208] (WB, Human)
Details:
phospho Ser724 - IRE1 alpha antibody used for WB (1:500 dilution, ON 4C incubation in 3% milk) on 20ug lysates of mucosal biopsy specimens collected from ileal and colonic district of inflammatory bowel disease paediatric patients and controls. See full text for details on tissue lysate preparation and WB analysis. Fig. 4 : WB image shows an increased pIRE expression in tissue lysates of inflamed colon of ulcerative colitis (UC) and Crohn's disease (CD) patients when compared to control subjects (Ctrl). |
WB |
Human |
| Kosakowska-Cholody T, Lin J, Srideshikan S M et al. HKH40A downregulates GRP78/BiP expression in cancer cells. Cell Death Dis. 2014-05-22 [PMID: 24853418] (WB) |
WB |
|
| Liew CW, Assmann A, Templin AT et al. Insulin regulates carboxypeptidase E by modulating translation initiation scaffolding protein eIF4G1 in pancreatic beta cells. Proc. Natl. Acad. Sci. U.S.A. 2014-05-19 [PMID: 24843127] (WB, Mouse) |
WB |
Mouse |
| Bonnet Wersinger D, Benkafadar N, Jagodzinska J et al. Impairment of Visual Function and Retinal ER Stress Activation in Wfs1-Deficient Mice. PLoS ONE. 2014-05-14 [PMID: 24823368] (IHC-Fr, Mouse) |
IHC-Fr |
Mouse |
| Cai XH, Li XC, Jin SW et al. Endoplasmic reticulum stress plays critical role in brain damage after chronic intermittent hypoxia in growing rats. Exp. Neurol. 2014-05-05 [PMID: 24810321] (WB, Rat) |
WB |
Rat |
| Hosoi T, Inoue Y, Nakatsu K et al. TERT attenuated ER stress-induced cell death. Biochem. Biophys. Res. Commun. 2014-04-16 [PMID: 24746472] (WB, Human) |
WB |
Human |
| Shao Mengle, Shan Bo, Liu Yang et al. Hepatic IRE1alpha regulates fasting-induced metabolic adaptive programs through the XBP1s-PPARalpha axis signalling. Nat Commun. 2014-03-27 [PMID: 24670948] (WB) |
WB |
|
| Koyama M, Furuhashi M et al. Reduction of endoplasmic reticulum stress by 4-phenylbutyric acid prevents the development of hypoxia-induced pulmonary arterial hypertension. Am J Physiol Heart Circ Physiol 2014-01-05 [PMID: 24610918] (WB, Human) |
WB |
Human |
| Suh HN, Lee YJ, Kim MO, Han HJ. Glucosamine-Induced Sp1 O-GlcNAcylation Ameliorates Hypoxia-Induced SGLT Dysfunction in Primary Cultured Renal Proximal Tubule Cells. J. Cell. Physiol. 2014-03-04 [PMID: 24591095] (WB, Rabbit) |
WB |
Rabbit |
| Lu G, Ota A, Ren S et al. PPM1l encodes an inositol requiring-protein 1 (IRE1) specific phosphatase that regulates the functional outcome of the ER stress response. Mol Metab 2013-01-01 [PMID: 24327956] |
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| Liu L, Fan Z, Tang Y, Ke Z. The Resveratrol Attenuates Ethanol-Induced Hepatocyte Apoptosis Via Inhibiting ER-Related Caspase-12 Activation and PDE Activity In Vitro. Alcohol Clin Exp Res. 2013-11-13 [PMID: 24224909] (WB, Human) |
WB |
Human |
| Zhao M, Zang B, Cheng M et al. Differential responses of hepatic endoplasmic reticulum stress and inflammation in diet-induced obese rats with high-fat diet rich in lard oil or soybean oil. PLoS One. [PMID: 24223162] (WB, Rat) |
WB |
Rat |
| Wang YI, Bettaieb A, Sun C et al. Triglyceride-rich lipoprotein modulates endothelial vascular cell adhesion molecule (VCAM)-1 expression via differential regulation of endoplasmic reticulum stress. PLoS One. [PMID: 24205197] (WB, Human) |
WB |
Human |
| Matsumoto H, Miyazaki S, Matsuyama S et al. Selection of autophagy or apoptosis in cells exposed to ER-stress depends on ATF4 expression pattern with or without CHOP expression. Biol Open. 2013-08-27 [PMID: 24167719] (WB, Human) |
WB |
Human |
| Jung DY, Chalasani U, Pan N et al. KLF15 Is a Molecular Link between Endoplasmic Reticulum Stress and Insulin Resistance. PLoS One. 2013-10-22 [PMID: 24167585] (WB, Mouse) |
WB |
Mouse |
| Omikorede O, Qi C, Gorman T et al. ER stress in rodent islets of Langerhans is concomitant with obesity and beta-cell compensation but not with beta-cell dysfunction and diabetes. Nutr Diabetes. 2013-10-21 [PMID: 24145577] (WB, Mouse) |
WB |
Mouse |
| Lim SK, Choi H, Park MJ et al. The ER stress-mediated decrease in DDAH1 expression is involved in formaldehyde-induced apoptosis in lung epithelial cells. Food Chem Toxicol. 2013-10-16 [PMID: 24140967] (WB, Human) |
WB |
Human |
| Yang C, Diiorio P, Jurczyk A et al. Pathological endoplasmic reticulum stress mediated by the IRE1 pathway contributes to pre-insulitic beta cell apoptosis in a virus-induced rat model of type 1 diabetes. Diabetologia 2013-12-01 [PMID: 24121653] (WB, Rat) |
WB |
Rat |
| Kemp KL, Lin Z, Zhao F et al. The Serine-threonine Kinase Inositol-requiring Enzyme 1alpha (IRE1alpha) Promotes IL-4 Production in T Helper Cells. J Biol Chem. 2013-11-15 [PMID: 24100031] (WB, Mouse) |
WB |
Mouse |
| Adolph TE, Tomczak MF, Niederreiter L et al. Paneth cells as a site of origin for intestinal inflammation. Nature. [PMID: 24089213] |
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|
| Chen YC, Zeng XY, He Y et al. Rutaecarpine Analogues Reduce Lipid Accumulation in Adipocytes via Inhibiting Adipogenesis/Lipogenesis with AMPK Activation and UPR Suppression. ACS Chem Biol. 2013-10-18 [PMID: 23962138] (WB, Mouse) |
WB |
Mouse |
| Kujiraoka T, Satoh Y, Ayaori M et al. Hepatic extracellular signal-regulated kinase 2 suppresses endoplasmic reticulum stress and protects from oxidative stress and endothelial dysfunction. J Am Heart Assoc. 2013-08-16 [PMID: 23954796] (WB, Mouse) |
WB |
Mouse |
| Stahl S, Burkhart JM, Hinte F et al. Cytomegalovirus Downregulates IRE1 to Repress the Unfolded Protein Response. PLoS Pathog. 2013-08-01 [PMID: 23950715] (WB, Human) |
WB |
Human |
| Xu B, Shan M, Wang F et al. Endoplasmic reticulum stress signaling involvement in manganese-induced nerve cell damage in organotypic brain slice cultures. Toxicol Lett. [PMID: 23948121] (WB, Rat) |
WB |
Rat |
| Kitai Y, Ariyama H, Kono N et al. Membrane lipid saturation activates IRE1alpha without inducing clustering. Genes Cells 2013-06-27 [PMID: 23803178] (WB, Human) |
WB |
Human |
| Song Y, Shen H, Du W, Goldstein DR. Inhibition of x-box binding protein 1 reduces tunicamycin-induced apoptosis in aged murine macrophages. Aging Cell 2013-05-28 [PMID: 23711292] (WB, Mouse) |
WB |
Mouse |
| Koh HJ, Toyoda T, Didesch MM et al. Tribbles 3 mediates endoplasmic reticulum stress-induced insulin resistance in skeletal muscle. Nat Commun 2013-01-01 [PMID: 23695665] (WB, Mouse) |
WB |
Mouse |
| Akiyama M, Liew CW, Lu S et al. X-box binding protein 1 is essential for insulin regulation of pancreatic alpha cell function Diabetes 2013-03-14 [PMID: 23493568] |
|
|
| Zheng M, Zhang Q, Joe Y et al. Curcumin induces apoptotic cell death of activated human CD4+ T cells via increasing endoplasmic reticulum stress and mitochondrial dysfunction. Int Immunopharmacol 2013-02-15 [PMID: 23415873] (WB, Human) |
WB |
Human |
| Lind KR, Ball KK, Cruz NF, Dienel GA. The unfolded protein response to endoplasmic reticulum stress in cultured astrocytes and rat brain during experimental diabetes. Neurochem Int. [PMID: 23411409] (WB, Rat) |
WB |
Rat |
| Arshad M, Ye Z, Gu X et al. RNF13, a RING Finger Protein, Mediates Endoplasmic Reticulum Stress-induced Apoptosis through the Inositol-requiring Enzyme (IRE1alpha)/c-Jun NH2-terminal Kinase Pathway. J Biol Chem 2013-03-22 [PMID: 23378536] (WB, Human) |
WB |
Human |
| Han J, Murthy R, Wood B et al. ER stress signalling through eIF2alpha and CHOP, but not IRE1alpha, attenuates adipogenesis in mice Diabetologia 2013-01-12 [PMID: 23314846] (WB, Mouse) |
WB |
Mouse |
| Otoda T, Takamura T, Misu H et al. Proteasome Dysfunction Mediates Obesity-Induced Endoplasmic Reticulum Stress and Insulin Resistance in the Liver Diabetes 2012-12-03 [PMID: 23209186] (WB, Mouse, Rat) |
WB |
Mouse, Rat |
| Cha SI, Ryerson CJ, Lee JS et al. Cleaved cytokeratin-18 is a mechanistically informative biomarker in idiopathic pulmonary fibrosis Respir Res 2012-11-20 [PMID: 23167970] (WB, Human) |
WB |
Human |
| Bartolome A, Guillen C, Benito M et al. Autophagy plays a protective role in endoplasmic reticulum stress-mediated pancreatic beta cell death. Autophagy 2012-12-01 [PMID: 22951927] |
|
|
| Ri M, Tashiro E, Oikawa D et al. Identification of Toyocamycin, an agent cytotoxic for multiple myeloma cells, as a potent inhibitor of ER stress-induced XBP1 mRNA splicing Blood Cancer J 2012-07-01 [PMID: 22852048] (WB, Human) |
WB |
Human |
| Kawabata S, Gills JJ, Mercado-Matos JR et al. Synergistic effects of nelfinavir and bortezomib on proteotoxic death of NSCLC and multiple myeloma cells. Cell Death Dis. [PMID: 22825471] (WB, Human) |
WB |
Human |
| Toosi S, Orlow SJ, Manga P. Vitiligo-Inducing Phenols Activate the Unfolded Protein Response in Melanocytes Resulting in Upregulation of IL6 and IL8 J Invest Dermatol 2012-06-14 [PMID: 22696056] (WB, Human) |
WB |
Human |
| Zheng X, Zheng X, Wang X et al. Acute hypoxia induces apoptosis of pancreatic B-cell by activation of the unfolded protein response and upregulation of CHOP. Cell Death Dis. 2012-06-14 [PMID: 22695615] (WB, Mouse) |
WB |
Mouse |
| Dickie LJ, Aziz AM, Savic S et al. Involvement of X-box binding protein 1 and reactive oxygen species pathways in the pathogenesis of tumour necrosis factor receptor-associated periodic syndrome. Ann Rheum Dis. [PMID: 22679299] |
|
|
| Jheng JR, Lin CY, Horng JT, Lau KS. Inhibition of enterovirus 71 entry by transcription factor XBP1. Biochem Biophys Res Commun. [PMID: 22469468] (WB, Human) |
WB |
Human |
| Zheng M, Kim SK, Joe Y, Back SH, Cho HR, Kim HP, Ignarro LJ, Chung HT. Sensing endoplasmic reticulum stress by protein kinase RNA-like endoplasmic reticulum kinase promotes adaptive mitochondrial DNA biogenesis and cell survival via heme oxygenase-1/carbon monoxide activity. FASEB J;26(6):2558-68. 2012-06-01 [PMID: 22391129] (WB, Mouse) |
WB |
Mouse |
| Wu LL, Russell DL, Norman RJ, Robker RL. Endoplasmic Reticulum (ER) Stress in Cumulus-Oocyte Complexes Impairs Pentraxin-3 Secretion, Mitochondrial Membrane Potential ({Delta}{Psi}m), and Embryo Development. Mol Endocrinol;26(4):562-73. 2012-04-01 [PMID: 22383462] (WB, Mouse) |
WB |
Mouse |
| Morcavallo A, Genua M, Palummo A et al. Insulin and insulin-like growth factor II differentially regulate endocytic sorting and stability of the insulin receptor isoform A J Biol Chem 2012-02-08 [PMID: 22318726] (ELISA, Mouse) |
ELISA |
Mouse |
| Ota A, Wang Y. Cdc37/Hsp90-mediated regulation of IRE1[alpha] activity in ER stress response and insulin synthesis in INS-1 cells. The Journal of Biological Chemistry. 2011-12-23 [PMID: 22199355] (WB, Human) |
WB |
Human |
| Teodoro T, Odisho T, Sidorova E, Volchuk A. Pancreatic beta-cells depend on basal expression of active ATF6?-p50 for cell survival even under nonstress conditions. Am J Physiol Cell Physiol;302(7):C992-C1003. 2012-04-01 [PMID: 22189555] (WB, Human) |
WB |
Human |
| Yamagishi N, Ueda T, Mori A et al. Decreased expression of endoplasmic reticulum chaperone GRP78 in liver of diabetic mice. Biochemical and Biophysical Research Communications. 2011-12-01 [PMID: 22155243] (Mouse) |
|
Mouse |
| Kimura K, Yamada T, Matsumoto M et al. Endoplasmic reticulum stress inhibits STAT3-dependent suppression of hepatic gluconeogenesis via dephosphorylation and deacetylation Diabetes 2012-01-01 [PMID: 22124464] (WB, Mouse) |
WB |
Mouse |
| Nijholt DA, van Haastert ES, Rozemuller AJ, Scheper W, Hoozemans JJ. The unfolded protein response is associated with early tau pathology in the hippocampus of tauopathies. J Pathol doi: 10.1002/path.3969. 2011-11-21 [PMID: 22102449] (IHC-P, Human) |
IHC-P |
Human |
| Wang C-T, Jetzt AE, Cheng J-S, Cohick WS. Inhibition of the unfolded protein response by ricin a-chain enhances its cytotoxicity in Mammalian cells. Toxins (Basel)3(5):453-68. 2011-05-01 [PMID: 22069719] (WB, Human) |
WB |
Human |
| Michallet A-S, Mondiere P, Taillardet M et al. Compromising the unfolded protein response induces autophagy-mediated cell death in multiple myeloma cells. PLoS ONE;6(10). 2011-01-01 [PMID: 22028791] (WB, Human) |
WB |
Human |
| Hage Hassan R, Hainault I, Vilquin J-T et al. Endoplasmic reticulum stress does not mediate palmitate-induced insulin resistance in mouse and human muscle cells. Diabetologia. 2011-10-18 [PMID: 22006247] (WB, Mouse) |
WB |
Mouse |
| Moumen A, Virard I, Raoul C. Accumulation of wildtype and ALS-linked mutated VAPB impairs activity of the proteasome. PLoS One. 1905-07-03 [PMID: 21998752] (WB, Primate) |
WB |
Primate |
| Shimada Y, Kobayashi H, Kawagoe S et al. Endoplasmic reticulum stress induces autophagy through activation of p38 MAPK in fibroblasts from Pompe disease patients carrying c. 546G>T mutation. Molecular Genetics and Metabolism. 2011-09-14 [PMID: 21982629] (WB, Human) |
WB |
Human |
| Merquiol E, Uzi D, Mueller T et al. HCV causes chronic endoplasmic reticulum stress leading to adaptation and interference with the unfolded protein response. PLoS One. 1905-07-03 [PMID: 21949742] (WB, Human) |
WB |
Human |
| Castillo K, Rojas-Rivera D, Lisbona F et al. BAX inhibitor-1 regulates autophagy by controlling the IRE1? branch of the unfolded protein response. The EMBO Journal. 2011-09-16 [PMID: 21926971] (WB, Mouse) |
WB |
Mouse |
| Chiang Chih-Kang, Hsu Shih-Ping, Wu Cheng-Tien et al. Endoplasmic reticulum stress implicated in the development of renal fibrosis. Mol Med. 2011-01-01 [PMID: 21863214] (WB, Rat) |
WB |
Rat |
| Chen K, Jin P, He HH et al. Overexpression of Insig-1 protects beta cell against glucolipotoxicity via SREBP-1c. J Biomed Sci 2011-01-01 [PMID: 21843373] (WB, Rat) |
WB |
Rat |
| Chuang T-C, Hsu S-C, Cheng Y-T et al. Magnolol down-regulates HER2 gene expression, leading to inhibition of HER2-mediated metastatic potential in ovarian cancer cells. Cancer Lett;311(1):11-9. 2011-12-01 [PMID: 21757288] |
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| Wang FM, Galson DL, Roodman GD, Ouyang H. Resveratrol triggers the pro-apoptotic endoplasmic reticulum stress response and represses pro-survival XBP1 signaling in human multiple myeloma cells. Exp Hematol. [PMID: 21723843] (WB, Human) |
WB |
Human |
| Lim MP, Devi LA, Rozenfeld R. Cannabidiol causes activated hepatic stellate cell death through a mechanism of endoplasmic reticulum stress-induced apoptosis. Cell Death Dis;2:e170. 2011-06-09 [PMID: 21654828] (WB, Rat, Mouse) |
WB |
Rat, Mouse |
| Oslowski CM, Urano F. Measuring ER stress and the unfolded protein response using mammalian tissue culture system. Methods Enzymol 2011-01-01 [PMID: 21266244] (Human) |
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Human |
| Zheng Z, Zhang C, Zhang K. Measurement of ER stress response and inflammation in the mouse model of nonalcoholic fatty liver disease. Methods Enzymol. 1905-07-03 [PMID: 21266239] (WB, Mouse) |
WB |
Mouse |
| Makioka K, Yamazaki T, Fujita Y et al. Involvement of endoplasmic reticulum stress defined by activated unfolded protein response in multiple system atrophy. J Neurol Sci 2010-10-15 [PMID: 20667553] (IF/IHC, Human) |
IF/IHC |
Human |
| Langou K, Moumen A, Pellegrino C et al. AAV-mediated expression of wild-type and ALS-linked mutant VAPB selectively triggers death of motoneurons through a Ca2+-dependent ER-associated pathway. J Neurochem. [PMID: 20477942] (WB, ICC/IF, Mouse) |
WB, ICC/IF |
Mouse |
| Winnay JN, Boucher J, Mori MA et al. A regulatory subunit of phosphoinositide 3-kinase increases the nuclear accumulation of X-box-binding protein-1 to modulate the unfolded protein response. Nat Med 2010-04-01 [PMID: 20348923] (Mouse) |
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Mouse |
| Qiu Y, Mao T, Zhang Y et al. A crucial role for RACK1 in the regulation of glucose-stimulated IRE1alpha activation in pancreatic beta cells. Sci Signal;3(106):ra7. 2010-01-26 [PMID: 20103773] (WB, Human) |
WB |
Human |
| Cretenet G, Le Clech M, Gachon F. Circadian clock-coordinated 12 Hr period rhythmic activation of the IRE1alpha pathway controls lipid metabolism in mouse liver. Cell Metab. [PMID: 20074527] (WB, Mouse) |
WB |
Mouse |
| Wang N, Zhang D, Mao X et al. Astragalus polysaccharides decreased the expression of PTP1B through relieving ER stress induced activation of ATF6 in a rat model of type 2 diabetes. Mol Cell Endocrinol. [PMID: 19524131] (WB, Rat) |
WB |
Rat |
| Hoozemans JJ, van Haastert ES, Nijholt DA et al. The Unfolded Protein Response Is Activated in Pretangle Neurons in Alzheimer';s Disease Hippocampus. Am J Pathol. 2009-03-05 [PMID: 19264902] (IHC-P, Human) |
IHC-P |
Human |
| Kashiwakura Y, Ochiai K, Watanabe M et al. Down-regulation of Inhibition of Differentiation-1 via Activation of Activating Transcription Factor 3 Smad Regulates REIC/Dickkopf-3-Induced Apoptosis. Cancer Res;68(20):8333-8341. 2008-01-01 [PMID: 18922905] (WB, Human) |
WB |
Human |
| Sugeno N, Takeda A, Hasegawa T et al. Serine 129 Phosphorylation of {alpha}-Synuclein Induces Unfolded Protein Response-mediated Cell Death. J Biol Chem;283(34):23179-23188. 2008-01-01 [PMID: 18562315] (WB, Human) |
WB |
Human |
| Luo D, He Y, Zhang H et al. AIP1 is critical in transducing IRE1-mediated endoplasmic reticulum stress response. J Biol Chem;283(18):11905-12. 2008-05-02 [PMID: 18281285] (WB, IP, Mouse) |
WB, IP |
Mouse |
| Lipson, KL et al. Regulation of insulin biosynthesis in pancreatic beta cells by an endoplasmic reticulum-resident protein kinase IER1. Cell Metabolism 4: 245-254. 2006-01-01 [PMID: 16950141] (WB, IP, Mouse, Rat, Human) |
WB, IP |
Mouse, Rat, Human |
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Show All 337 Publications.
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![IRE1 alpha [p Ser724] Antibody - BSA Free](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "IRE1 alpha [p Ser724] Antibody - BSA Free")
![Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - ABT-263 induces ER stress & activation of the unfolded protein response.a Western blot of ER stress molecules in uveal melanoma cells treated with ABT-263 5 μM for 24 h & 48 h. HSP90 is used as a loading control. Representative western blots are shown. b XBP1 splicing in uveal melanoma cells exposed to ABT-263 5 μM for 15 h. *P-value < 0.05, **P-value < 0.01. c Kinetic analysis of ER stress proteins in Mel270 cells treated with ABT-263 5 μM in the absence or presence of qVD-OPh 20 μM. HSP90 is used as a loading control. Representative western blots are shown. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32337074), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2323_rabbit-polyclonal-ire1-alpha-p-ser724-antibody-310202415175222.jpg "Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - ABT-263 induces ER stress & activation of the unfolded protein response.a Western blot of ER stress molecules in uveal melanoma cells treated with ABT-263 5 μM for 24 h & 48 h. HSP90 is used as a loading control. Representative western blots are shown. b XBP1 splicing in uveal melanoma cells exposed to ABT-263 5 μM for 15 h. *P-value < 0.05, **P-value < 0.01. c Kinetic analysis of ER stress proteins in Mel270 cells treated with ABT-263 5 μM in the absence or presence of qVD-OPh 20 μM. HSP90 is used as a loading control. Representative western blots are shown. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32337074), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - Effect of cancer on ER stress markers.Bip1, IRE-1, ATF-6 p50 & CHOP expression in the liver of non—cachectic ApcMin/+ mice (N = 6 per group), compared to healthy C57BL/6 mice. Dotted line on the western blot indicates two different sections of the same gel. Values are expressed as Mean ± SE. * denotes significantly different from the healthy C57BL/6 mice as analyzed by a pre—planned t—test. p < 0.05. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0119888), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2323_rabbit-polyclonal-ire1-alpha-p-ser724-antibody-310202415384120.jpg "Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - Effect of cancer on ER stress markers.Bip1, IRE-1, ATF-6 p50 & CHOP expression in the liver of non—cachectic ApcMin/+ mice (N = 6 per group), compared to healthy C57BL/6 mice. Dotted line on the western blot indicates two different sections of the same gel. Values are expressed as Mean ± SE. * denotes significantly different from the healthy C57BL/6 mice as analyzed by a pre—planned t—test. p < 0.05. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0119888), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - ER stress is induced by acidosis activated OGR1-mediated signalling. Caco-2 cells subjected to different pH medium, following 4–6 h incubation in pH 7.6 serum free medium.(B) After 24 h pH shift, total protein was isolated & WB was performed. The results representative of 3 independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31996710), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2323_rabbit-polyclonal-ire1-alpha-p-ser724-antibody-31020241536371.jpg "Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - ER stress is induced by acidosis activated OGR1-mediated signalling. Caco-2 cells subjected to different pH medium, following 4–6 h incubation in pH 7.6 serum free medium.(B) After 24 h pH shift, total protein was isolated & WB was performed. The results representative of 3 independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31996710), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - Expression of ER stress markers in mouse & human skeletal muscle(a) TA muscle lysates from high fat fed mice were used to determine the expression of ER stress markers by Western blot analysis (n=6). (b-g) Western blot analysis was done to measure proteins involved in ER stress in vastus lateralis muscle from lean, obese, or diabetic subjects (n=6-13). Data are the means ± S.E.M. * indicates p<0.05 & ** indicates p<0.01 vs. chow or lean controls, using Student’s t-test (a) & One way ANOVA. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms2851), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2323_rabbit-polyclonal-ire1-alpha-p-ser724-antibody-310202415175214.jpg "Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - Expression of ER stress markers in mouse & human skeletal muscle(a) TA muscle lysates from high fat fed mice were used to determine the expression of ER stress markers by Western blot analysis (n=6). (b-g) Western blot analysis was done to measure proteins involved in ER stress in vastus lateralis muscle from lean, obese, or diabetic subjects (n=6-13). Data are the means ± S.E.M. * indicates p<0.05 & ** indicates p<0.01 vs. chow or lean controls, using Student’s t-test (a) & One way ANOVA. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms2851), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - The inositol-requiring enzyme 1 alpha (IRE1 alpha )/ X-box-binding-protein 1 spliced isoform (XBP1s) arm of unfolded protein response (UPR) is induced by angiotensin II in VSMCs. (A–C) The rat aortic VSMCs infected with adenovirus encoding GRP78 or control GFP (100 moi) for 48 h were stimulated with 100 nM AngII (AII) for 1–6 h & immunoblotting was performed as indicated. (A) Representative blots from 4 independent experiments. (B) Signal intensity was used to calculate the expression ratio of XBP1s to GAPDH. (C) Signal intensity was used to calculate the IRE1 alpha Ser724 phosphorylation ratio to the total IRE1 alpha . The bars in the graphs show the mean ± SD from 4 independent experiments. * indicates p < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32679678), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2323_rabbit-polyclonal-ire1-alpha-p-ser724-antibody-310202415291930.jpg "Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - The inositol-requiring enzyme 1 alpha (IRE1 alpha )/ X-box-binding-protein 1 spliced isoform (XBP1s) arm of unfolded protein response (UPR) is induced by angiotensin II in VSMCs. (A–C) The rat aortic VSMCs infected with adenovirus encoding GRP78 or control GFP (100 moi) for 48 h were stimulated with 100 nM AngII (AII) for 1–6 h & immunoblotting was performed as indicated. (A) Representative blots from 4 independent experiments. (B) Signal intensity was used to calculate the expression ratio of XBP1s to GAPDH. (C) Signal intensity was used to calculate the IRE1 alpha Ser724 phosphorylation ratio to the total IRE1 alpha . The bars in the graphs show the mean ± SD from 4 independent experiments. * indicates p < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32679678), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - Morphine suppressed 6-OHDA-induced ER stress through activation of UPR. (A,B) Morphine induced UPR in SH-SY5Y cells. Protein levels of GRP78, p-IRE1 alpha , IRE1 alpha , p-PERK, PERK, ATF6, CHOP & Tubulin in SH-SY5Y cells were analyzed (A) & quantified (B) by western blot. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001 vs. control. (C,D) Protein levels of GRP78, CHOP & Actin in SH-SY5Y cells were analyzed (C) & quantified (D) by western blot in the indicated groups. ∗P < 0.05, ∗∗P < 0.01 vs. control & #P < 0.05, ##P < 0.01 vs. 6-OHDA-treated group. All data are expressed as mean ± SEM. Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fnmol.2018.00120/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2323_rabbit-polyclonal-ire1-alpha-p-ser724-antibody-310202415171313.jpg "Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - Morphine suppressed 6-OHDA-induced ER stress through activation of UPR. (A,B) Morphine induced UPR in SH-SY5Y cells. Protein levels of GRP78, p-IRE1 alpha , IRE1 alpha , p-PERK, PERK, ATF6, CHOP & Tubulin in SH-SY5Y cells were analyzed (A) & quantified (B) by western blot. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001 vs. control. (C,D) Protein levels of GRP78, CHOP & Actin in SH-SY5Y cells were analyzed (C) & quantified (D) by western blot in the indicated groups. ∗P < 0.05, ∗∗P < 0.01 vs. control & #P < 0.05, ##P < 0.01 vs. 6-OHDA-treated group. All data are expressed as mean ± SEM. Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fnmol.2018.00120/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - Hepatic ER Stress.Representative hepatic western blots of BiP, phosphorylated (Ser51), total EIF2 alpha & phosphorylated:total EIF2 alpha , phosphorylated (Ser724), total IRE1 alpha & phosphorylated:total IRE1 alpha , & CHOP (n = 9). Diets not sharing a common letter differ significantly from one another (P≤.05). Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0167979), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2323_rabbit-polyclonal-ire1-alpha-p-ser724-antibody-310202415284519.jpg "Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - Hepatic ER Stress.Representative hepatic western blots of BiP, phosphorylated (Ser51), total EIF2 alpha & phosphorylated:total EIF2 alpha , phosphorylated (Ser724), total IRE1 alpha & phosphorylated:total IRE1 alpha , & CHOP (n = 9). Diets not sharing a common letter differ significantly from one another (P≤.05). Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0167979), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - Effect of Insig-1 overexpression on nSREBP-1c & proteins related to ER stress pathway in INS-1 cells. INS-1 & INS-1-Insig-1 cells were stimulated with 11.2 mM & 25.0 mM glucose for 0, 24 & 72 h. A) nSREBP-1c & p-IRE1 alpha pathway of ER stress related proteins were detected by Western blot. GAPDH was used as control for protein loading. Equal amount of proteins (50 μg) were loaded. B) Relative protein expression compared to GAPDH, data are the means ± SE of three independent experiments; *, P < 0.05, **, P < 0.01 VS INS-1 cells at the same time point. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21843373), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2323_rabbit-polyclonal-ire1-alpha-p-ser724-antibody-31020241535662.jpg "Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - Effect of Insig-1 overexpression on nSREBP-1c & proteins related to ER stress pathway in INS-1 cells. INS-1 & INS-1-Insig-1 cells were stimulated with 11.2 mM & 25.0 mM glucose for 0, 24 & 72 h. A) nSREBP-1c & p-IRE1 alpha pathway of ER stress related proteins were detected by Western blot. GAPDH was used as control for protein loading. Equal amount of proteins (50 μg) were loaded. B) Relative protein expression compared to GAPDH, data are the means ± SE of three independent experiments; *, P < 0.05, **, P < 0.01 VS INS-1 cells at the same time point. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21843373), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - cCK-18 is formed following activation of the UPR. A. Immunoblots for phospho-IRE1 (p-IRE1), total IRE1 (IRE1), & cleaved cytokeratin 18 (cCK-18) showing time-dependent increase of cCK-18 in A549 cells exposed to 0.5 μM of thapsigargin (TG, +) for various lengths of time. Immunoblots showing an increase in total & phospho-IRE1 confirm thapsigargin activates the UPR in A549 cells compared to vehicle controls (−). B. Immunoblots for cCK-18 showing time-dependent appearance of cCK-18 in annexin V– or V+ A549 cells exposed to 0.5 μM of thapsigargin for various lengths of time. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23167970), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2323_rabbit-polyclonal-ire1-alpha-p-ser724-antibody-310202415175288.jpg "Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - cCK-18 is formed following activation of the UPR. A. Immunoblots for phospho-IRE1 (p-IRE1), total IRE1 (IRE1), & cleaved cytokeratin 18 (cCK-18) showing time-dependent increase of cCK-18 in A549 cells exposed to 0.5 μM of thapsigargin (TG, +) for various lengths of time. Immunoblots showing an increase in total & phospho-IRE1 confirm thapsigargin activates the UPR in A549 cells compared to vehicle controls (−). B. Immunoblots for cCK-18 showing time-dependent appearance of cCK-18 in annexin V– or V+ A549 cells exposed to 0.5 μM of thapsigargin for various lengths of time. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23167970), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - Protein expression of downstream markers of ER stress in islets from lean, obese & high-fat-diet-fed fZDF rats. Protein lysates from islets isolated from three age-matched fZDF rats (obese), HF-fZDF (HFD) rats & their heterozygous lean littermates (lean) were separated by SDS-PAGE & analysed by western blotting. As controls, lysates from the islets isolated from Wistar rats or MIN6 cells treated with 1 μM thapsigargin for 2 h or sorbitol for 1 h were run alongside. Proteins were detected using antisera against (a) Calreticulin, HYOU1, GRP78, p-IRE1 alpha , p-eIF2 alpha & CHOP & as protein loading control rpS6 & (b) p-JNK & total JNK1/2 as loading control. Western blots were quantified using Image-J software. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/nutd201335), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2323_rabbit-polyclonal-ire1-alpha-p-ser724-antibody-310202415175287.jpg "Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - Protein expression of downstream markers of ER stress in islets from lean, obese & high-fat-diet-fed fZDF rats. Protein lysates from islets isolated from three age-matched fZDF rats (obese), HF-fZDF (HFD) rats & their heterozygous lean littermates (lean) were separated by SDS-PAGE & analysed by western blotting. As controls, lysates from the islets isolated from Wistar rats or MIN6 cells treated with 1 μM thapsigargin for 2 h or sorbitol for 1 h were run alongside. Proteins were detected using antisera against (a) Calreticulin, HYOU1, GRP78, p-IRE1 alpha , p-eIF2 alpha & CHOP & as protein loading control rpS6 & (b) p-JNK & total JNK1/2 as loading control. Western blots were quantified using Image-J software. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/nutd201335), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - CETP inhibition reduces endoplasmic reticulum stress. HAECs transfected with CETP or control scrambled siRNA. (A) Western blot analysis of PERK (n = 3, * p < 0.01) & qPCR analysis of ATF6 mRNA. (B) Phosphorylated & total IRE (n = 3, ns). (C) Representative microscopic images of HAECs stained with GADD153, n = 5, & (D) Grp78/BIP, & DAPI. Scale bar, 10 μm. Mean ± SEM, n = 5, * p < 0.05. HAECs, human aortic endothelial cells. CETP, cholesteryl ester transfer protein. NS, non-silenced. siCETP, silenced CETP. PERK, protein kinase RNA-like ER kinase. ATF6, Activating transcription factor 6. IRE, inhibitor resistant esterase. BIP, binding immunoglobulin protein. GADD153, growth arrest- & DNA damage-inducible gene 153. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33430172), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2323_rabbit-polyclonal-ire1-alpha-p-ser724-antibody-31020241529334.jpg "Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - CETP inhibition reduces endoplasmic reticulum stress. HAECs transfected with CETP or control scrambled siRNA. (A) Western blot analysis of PERK (n = 3, * p < 0.01) & qPCR analysis of ATF6 mRNA. (B) Phosphorylated & total IRE (n = 3, ns). (C) Representative microscopic images of HAECs stained with GADD153, n = 5, & (D) Grp78/BIP, & DAPI. Scale bar, 10 μm. Mean ± SEM, n = 5, * p < 0.05. HAECs, human aortic endothelial cells. CETP, cholesteryl ester transfer protein. NS, non-silenced. siCETP, silenced CETP. PERK, protein kinase RNA-like ER kinase. ATF6, Activating transcription factor 6. IRE, inhibitor resistant esterase. BIP, binding immunoglobulin protein. GADD153, growth arrest- & DNA damage-inducible gene 153. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33430172), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - Activation of UPR signaling pathways after UIRI. a & b The expression of p-PERK & t-PERK in kidneys of UIRI mice were evaluated with western blot analysis & quantified. c UIRI induces the activation of ATF6 as demonstrated by enhanced expression of cleaved ATF6 (cATF6). GAPDH was used as an internal control. d Fold-change expression of cATF6 in kidneys of UIRI mouse as compared with that of sham group mouse. e & f The expression of p-IRE1 & t-IRE1 in mice kidneys were evaluated with western blotting & quantified. Data are expressed as means ± SEM, n = 3 ~ 6 in each group. *P < 0.05, **P < 0.01, & ***P < 0.001, as compared with sham group Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35765067), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2323_rabbit-polyclonal-ire1-alpha-p-ser724-antibody-310202415371925.jpg "Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - Activation of UPR signaling pathways after UIRI. a & b The expression of p-PERK & t-PERK in kidneys of UIRI mice were evaluated with western blot analysis & quantified. c UIRI induces the activation of ATF6 as demonstrated by enhanced expression of cleaved ATF6 (cATF6). GAPDH was used as an internal control. d Fold-change expression of cATF6 in kidneys of UIRI mouse as compared with that of sham group mouse. e & f The expression of p-IRE1 & t-IRE1 in mice kidneys were evaluated with western blotting & quantified. Data are expressed as means ± SEM, n = 3 ~ 6 in each group. *P < 0.05, **P < 0.01, & ***P < 0.001, as compared with sham group Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35765067), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - Apoptosis mediated by Arl6ip5 interference is regulated by Chop. (a) Immunoblotting was used to detect the protein level for p-IRE1 alpha , IRE1, Chop, Arl6ip5 & beta -actin in the UAMS-32 cell with NC-siRNA or Arl6ip5-siRNA treatment for 72 h. Representative results were shown from three independent experiments. (b–e) UAMS-32 cells first received NC-siRNA or Chop-siRNA for 24 h, then the medium was changed & further received NC-siRNA or Arl6ip5-siRNA for 72 h. Q-PCR was used to analyze the mRNA levels of Chop (b), GADD34 (c) & Trib3 (d). The apoptotic cells were stained with Annexin-V & the proportion were calculated (e). n=4. In all panels, error bars represent the mean±S.E.M. **P<0.01, *P<0.05 based on ANOVA Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/cddis2014427), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2323_rabbit-polyclonal-ire1-alpha-p-ser724-antibody-310202415392557.jpg "Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - Apoptosis mediated by Arl6ip5 interference is regulated by Chop. (a) Immunoblotting was used to detect the protein level for p-IRE1 alpha , IRE1, Chop, Arl6ip5 & beta -actin in the UAMS-32 cell with NC-siRNA or Arl6ip5-siRNA treatment for 72 h. Representative results were shown from three independent experiments. (b–e) UAMS-32 cells first received NC-siRNA or Chop-siRNA for 24 h, then the medium was changed & further received NC-siRNA or Arl6ip5-siRNA for 72 h. Q-PCR was used to analyze the mRNA levels of Chop (b), GADD34 (c) & Trib3 (d). The apoptotic cells were stained with Annexin-V & the proportion were calculated (e). n=4. In all panels, error bars represent the mean±S.E.M. **P<0.01, *P<0.05 based on ANOVA Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/cddis2014427), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - Downregulation of gamma -taxilin & alpha NAC, & ER stress responses in mouse brain slice cultured under hypoxic conditions. (a) Immunohistochemistry shows downregulation of gamma -taxilin & alpha NAC expression in brain slices cultured for 48 h under hypoxic conditions. Scale bar, 20 μm. (b) Western blot shows downregulation of gamma -taxilin & alpha NAC, phosphorylation of PERK & IRE1 alpha & induction of CHOP in mouse brain slices cultured for 48 h under normoxic (−) or hypoxic conditions (+) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25880086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2323_rabbit-polyclonal-ire1-alpha-p-ser724-antibody-310202415304295.jpg "Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - Downregulation of gamma -taxilin & alpha NAC, & ER stress responses in mouse brain slice cultured under hypoxic conditions. (a) Immunohistochemistry shows downregulation of gamma -taxilin & alpha NAC expression in brain slices cultured for 48 h under hypoxic conditions. Scale bar, 20 μm. (b) Western blot shows downregulation of gamma -taxilin & alpha NAC, phosphorylation of PERK & IRE1 alpha & induction of CHOP in mouse brain slices cultured for 48 h under normoxic (−) or hypoxic conditions (+) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25880086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - Differential ER stress response pathways initiated by gamma -taxilin or alpha NAC depletion. Proteins involved in UPR sensor (PERK, IRE1 alpha ), eIF2 alpha , JNK, Bcl-2, & Bcl-2-related protein (Puma, Bim, Bak, & Bax) signaling pathways were analyzed by Western blotting of HeLa S3 cells that were depleted of gamma -taxilin or alpha NAC by RNA intereference for 72 & 96 h. Upper panels, taxilin & NAC proteins; middle panels, UPR proteins; lower panels; apoptotic proteins; & bottom panel, beta -actin Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25880086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2323_rabbit-polyclonal-ire1-alpha-p-ser724-antibody-310202415334944.jpg "Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - Differential ER stress response pathways initiated by gamma -taxilin or alpha NAC depletion. Proteins involved in UPR sensor (PERK, IRE1 alpha ), eIF2 alpha , JNK, Bcl-2, & Bcl-2-related protein (Puma, Bim, Bak, & Bax) signaling pathways were analyzed by Western blotting of HeLa S3 cells that were depleted of gamma -taxilin or alpha NAC by RNA intereference for 72 & 96 h. Upper panels, taxilin & NAC proteins; middle panels, UPR proteins; lower panels; apoptotic proteins; & bottom panel, beta -actin Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25880086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - Expression of ER stress markers in mouse & human skeletal muscle(a) TA muscle lysates from high fat fed mice were used to determine the expression of ER stress markers by Western blot analysis (n=6). (b-g) Western blot analysis was done to measure proteins involved in ER stress in vastus lateralis muscle from lean, obese, or diabetic subjects (n=6-13). Data are the means ± S.E.M. * indicates p<0.05 & ** indicates p<0.01 vs. chow or lean controls, using Student’s t-test (a) & One way ANOVA. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms2851), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2323_rabbit-polyclonal-ire1-alpha-p-ser724-antibody-310202415384182.jpg "Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - Expression of ER stress markers in mouse & human skeletal muscle(a) TA muscle lysates from high fat fed mice were used to determine the expression of ER stress markers by Western blot analysis (n=6). (b-g) Western blot analysis was done to measure proteins involved in ER stress in vastus lateralis muscle from lean, obese, or diabetic subjects (n=6-13). Data are the means ± S.E.M. * indicates p<0.05 & ** indicates p<0.01 vs. chow or lean controls, using Student’s t-test (a) & One way ANOVA. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms2851), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - ER stress is induced by acidosis activated OGR1-mediated signalling. Caco-2 cells subjected to different pH medium, following 4–6 h incubation in pH 7.6 serum free medium. (F) A specific small molecule OGR1 inhibitor (10 µM) was tested & the cells subjected to low pH for 24 h, following 4–6 h incubation in pH 7.6 serum free medium. After 24 h pH shift, total protein was isolated & WB performed. Results representative of two independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31996710), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2323_rabbit-polyclonal-ire1-alpha-p-ser724-antibody-31020241529194.jpg "Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - ER stress is induced by acidosis activated OGR1-mediated signalling. Caco-2 cells subjected to different pH medium, following 4–6 h incubation in pH 7.6 serum free medium. (F) A specific small molecule OGR1 inhibitor (10 µM) was tested & the cells subjected to low pH for 24 h, following 4–6 h incubation in pH 7.6 serum free medium. After 24 h pH shift, total protein was isolated & WB performed. Results representative of two independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31996710), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - The UPR is activated in type II AECs isolated from IPF lungs. Phosphorylated IRE-1 alpha (panel A) & spliced XBP-1 (panel B) were present in increased amounts in AECs from 3 different IPF lungs, but not in the 3 control subjects (* P < 0.05, **P = 0.0495 by Wilcoxon rank sum test). # indicates amplicon of unspliced XBP1 digested with Pst1. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23167970), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2323_rabbit-polyclonal-ire1-alpha-p-ser724-antibody-31020241537195.jpg "Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - The UPR is activated in type II AECs isolated from IPF lungs. Phosphorylated IRE-1 alpha (panel A) & spliced XBP-1 (panel B) were present in increased amounts in AECs from 3 different IPF lungs, but not in the 3 control subjects (* P < 0.05, **P = 0.0495 by Wilcoxon rank sum test). # indicates amplicon of unspliced XBP1 digested with Pst1. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23167970), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - ER stress is induced by OGR1 via IRE1 alpha /JNK signalling. (A) Caco-2 cells were subjected to different pH medium, with or without an OGR1 inhibitor (10 µM), following 4–6 h in pH 7.6 serum free medium. After 24 h pH shift, total protein was isolated & Western blot performed. Results are representative of two independent experiments. (B) Caco-2 cells were subjected to different pH medium After 24 h pH shift, total protein was isolated & Western blot performed. Results are representative of two independent experiments. (C) Caco-2 cells were subjected to different pH medium with or without a JNK inhibitor (10 µM) following 4–6 h in pH 7.6 serum free medium. After 24 h pH shift, total protein was isolated & Western blot performed. Results are representative of two independent experiments. (D) Caco-2 cells were starved & subjected to an acidic pH with or without a JNK inhibitor as described in (C). After 24 h pH shift, total RNA was isolated & mRNA expression was investigated by qPCR. Statistical analysis was performed using one-way ANOVA followed by Tukey’s post-test. Data are presented as means ± SE of three independent experiments (***p < 0.001). (E) Caco-2 cells were starved & subjected to different pH medium following 4–6 h in pH 7.6 serum free medium. After 24 h pH shift, total protein was isolated & co-IP using IRE1 alpha antibody & JNK antibody was performed, followed by immunoblotting. Results are representative of two independent experiments. pH conditions: High pH 7.5–7.8; Normal pH 7.2–7.4; Low pH 6.6–6.8. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31996710), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2323_rabbit-polyclonal-ire1-alpha-p-ser724-antibody-310202415535198.jpg "Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - ER stress is induced by OGR1 via IRE1 alpha /JNK signalling. (A) Caco-2 cells were subjected to different pH medium, with or without an OGR1 inhibitor (10 µM), following 4–6 h in pH 7.6 serum free medium. After 24 h pH shift, total protein was isolated & Western blot performed. Results are representative of two independent experiments. (B) Caco-2 cells were subjected to different pH medium After 24 h pH shift, total protein was isolated & Western blot performed. Results are representative of two independent experiments. (C) Caco-2 cells were subjected to different pH medium with or without a JNK inhibitor (10 µM) following 4–6 h in pH 7.6 serum free medium. After 24 h pH shift, total protein was isolated & Western blot performed. Results are representative of two independent experiments. (D) Caco-2 cells were starved & subjected to an acidic pH with or without a JNK inhibitor as described in (C). After 24 h pH shift, total RNA was isolated & mRNA expression was investigated by qPCR. Statistical analysis was performed using one-way ANOVA followed by Tukey’s post-test. Data are presented as means ± SE of three independent experiments (***p < 0.001). (E) Caco-2 cells were starved & subjected to different pH medium following 4–6 h in pH 7.6 serum free medium. After 24 h pH shift, total protein was isolated & co-IP using IRE1 alpha antibody & JNK antibody was performed, followed by immunoblotting. Results are representative of two independent experiments. pH conditions: High pH 7.5–7.8; Normal pH 7.2–7.4; Low pH 6.6–6.8. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31996710), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - Downregulation of gamma -taxilin & alpha NAC & ER stress responses in hypoxic cells. (a) Phase-contrast micrographs of SK-N-SH cells cultures in hypoxic conditions for 0–48 h. Floating cells were evident by 37 h after the beginning of hypoxic treatment. (b) FACS (fluorescence-activated cell sorting) analysis of apoptosis in hypoxic SK-N-SH cells. Bars in the FACS profiles (left panel) indicate the fraction locations of annexin-positive cells. Bar graph indicates the relative numbers of annexin-positive cells (right panel). Bar graph data are shown as means±S.D. (n=3). *Significantly different from controls (0 h) (P<0.001, Tukey–Kramer test). (c) DAPI (4',6-diamidino-2-phenylindole) staining shows fragmented nuclei of apoptotic SK-N-SH cells after hypoxic treatment. Scale bar, 10 μm. (d) Downregulation of gamma -taxilin & alpha NAC & induction of ER stress responses in hypoxic SK-N-SH cells. Western blot analysis was performed 0 to 48 h after hypoxic treatment of cells. Upper panels, taxilin & NAC proteins; middle panels, UPR proteins; lower panels, apoptotic proteins; & bottom panels, cleaved caspase-9 & beta -actin proteins. (e) MG-132 treatment restore the gamma -taxilin & alpha NAC protein level in hypoxic (16 h) HeLa S3 cells. MG-132 (−), DMSO; MG-132 (+), 20 μM. (f) Caspase inhibition does not affect the hypoxia-induced downregulation of gamma -taxilin & alpha NAC in HeLa S3 cells. (g) Colocalization of gamma -taxilin & alpha NAC in normoxic (0 h) & hypoxic (8 h) HeLa S3 cells. Note that the colocalization fraction increases with the appearance of apoptotic phenotypes. Scale bars, 10 μm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25880086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2323_rabbit-polyclonal-ire1-alpha-p-ser724-antibody-310202415535172.jpg "Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - Downregulation of gamma -taxilin & alpha NAC & ER stress responses in hypoxic cells. (a) Phase-contrast micrographs of SK-N-SH cells cultures in hypoxic conditions for 0–48 h. Floating cells were evident by 37 h after the beginning of hypoxic treatment. (b) FACS (fluorescence-activated cell sorting) analysis of apoptosis in hypoxic SK-N-SH cells. Bars in the FACS profiles (left panel) indicate the fraction locations of annexin-positive cells. Bar graph indicates the relative numbers of annexin-positive cells (right panel). Bar graph data are shown as means±S.D. (n=3). *Significantly different from controls (0 h) (P<0.001, Tukey–Kramer test). (c) DAPI (4',6-diamidino-2-phenylindole) staining shows fragmented nuclei of apoptotic SK-N-SH cells after hypoxic treatment. Scale bar, 10 μm. (d) Downregulation of gamma -taxilin & alpha NAC & induction of ER stress responses in hypoxic SK-N-SH cells. Western blot analysis was performed 0 to 48 h after hypoxic treatment of cells. Upper panels, taxilin & NAC proteins; middle panels, UPR proteins; lower panels, apoptotic proteins; & bottom panels, cleaved caspase-9 & beta -actin proteins. (e) MG-132 treatment restore the gamma -taxilin & alpha NAC protein level in hypoxic (16 h) HeLa S3 cells. MG-132 (−), DMSO; MG-132 (+), 20 μM. (f) Caspase inhibition does not affect the hypoxia-induced downregulation of gamma -taxilin & alpha NAC in HeLa S3 cells. (g) Colocalization of gamma -taxilin & alpha NAC in normoxic (0 h) & hypoxic (8 h) HeLa S3 cells. Note that the colocalization fraction increases with the appearance of apoptotic phenotypes. Scale bars, 10 μm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25880086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - gamma -Taxilin ablation causes ER stress responses & apoptosis in normoxic cells. (a) Knockdown of gamma -taxilin expression by small interfering RNA (siRNA) in HeLa S3 cells. Two different siRNA constructs are equally effective. HeLa S3 cells were treated with solvent alone (Mock), control siRNA (Co), or gamma -taxilin siRNA ( gamma -tax-1 or -2) for 96 h. (b) Induction of apoptosis by gamma -taxilin ablation in HeLa S3 cells. gamma -Taxilin ablation causes apoptosis in HeLa S3 cells, but Mock or Co treatment did not. Bar graph shows fractions of annexin-positive cells (means±S.D., n=3). *Significantly different from Mock- or Co treatment (P <0.001, Tukey–Kramer test). (c) Confocal microscopy demonstrates coincidence of gamma -taxilin depletion & apoptotic nuclei in HeLa S3 cells treated with gamma -taxilin siRNA. Scale bar, 10 μm. (d) gamma -Taxilin depletion triggers ER stress responses in HeLa S3 cells. Upper panels, taxilin & NAC proteins; middle panels, UPR proteins; & lower panel, beta -actin. (e) gamma -Taxilin ablation induces accumulation of ubiquitinated proteins in HeLa S3 cells. Cell lysates were analyzed on 7.5% SDS-PAGE, followed by immunoblotting with antibodies specific for anti-mono- & polyubiquitinated conjugates (upper panel) & beta -actin (lower panel) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25880086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2323_rabbit-polyclonal-ire1-alpha-p-ser724-antibody-31020241682191.jpg "Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - gamma -Taxilin ablation causes ER stress responses & apoptosis in normoxic cells. (a) Knockdown of gamma -taxilin expression by small interfering RNA (siRNA) in HeLa S3 cells. Two different siRNA constructs are equally effective. HeLa S3 cells were treated with solvent alone (Mock), control siRNA (Co), or gamma -taxilin siRNA ( gamma -tax-1 or -2) for 96 h. (b) Induction of apoptosis by gamma -taxilin ablation in HeLa S3 cells. gamma -Taxilin ablation causes apoptosis in HeLa S3 cells, but Mock or Co treatment did not. Bar graph shows fractions of annexin-positive cells (means±S.D., n=3). *Significantly different from Mock- or Co treatment (P <0.001, Tukey–Kramer test). (c) Confocal microscopy demonstrates coincidence of gamma -taxilin depletion & apoptotic nuclei in HeLa S3 cells treated with gamma -taxilin siRNA. Scale bar, 10 μm. (d) gamma -Taxilin depletion triggers ER stress responses in HeLa S3 cells. Upper panels, taxilin & NAC proteins; middle panels, UPR proteins; & lower panel, beta -actin. (e) gamma -Taxilin ablation induces accumulation of ubiquitinated proteins in HeLa S3 cells. Cell lysates were analyzed on 7.5% SDS-PAGE, followed by immunoblotting with antibodies specific for anti-mono- & polyubiquitinated conjugates (upper panel) & beta -actin (lower panel) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25880086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - Localization of the WT & mutant Wrn proteins in different subcellular fractions.(A) Example of western blots showing the presence of the Wrn WT protein in the nuclear fraction & the presence of the Wrn delta hel mutant protein mainly in the cytosolic fraction. Fractionations were performed on WT, Wrn delta hel/ delta hel, & Wrn-/- MEFs. Topoisomerase I & beta -tubulin are nuclear & cytoplasmic markers, respectively. As expected the molecular weight of the Wrn delta hel mutant protein was lower than the Wrn WT protein. No band was detected in Wrn-/- MEFs fractions with the anti-Wrn polyclonal antibody. (B) Example of western blots showing only the presence of the Wrn delta hel mutant protein in total ER & the peroxisomal fractions. Fractionations were performed on WT, Wrn delta hel/ delta hel, & Wrn-/- liver tissues. Catalase & calreticulin are used as peroxisomal & ER specific markers. This experiment was repeated three times. (C) Protein levels of GRP78, phosphorylated IRE alpha , & beta -actin (top panels) & of total IRE alpha , & beta -actin (bottom panels) in the spleen of three animals of each genotype. (D) Ratio of GRP78 signal over beta -actin signal from western blots. (E) Ratio of IRE alpha over beta -actin signal from western blots. (F) Ratio of phosphorylated IRE alpha signal over beta -actin signal from western blots. Experiments were performed in triplicates. Bars in all histograms represent SEM. Tukey post ANOVA test P-values are shown (*P < 0.05 & **P < 0.01). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26447695), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2323_rabbit-polyclonal-ire1-alpha-p-ser724-antibody-31020241682134.jpg "Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - Localization of the WT & mutant Wrn proteins in different subcellular fractions.(A) Example of western blots showing the presence of the Wrn WT protein in the nuclear fraction & the presence of the Wrn delta hel mutant protein mainly in the cytosolic fraction. Fractionations were performed on WT, Wrn delta hel/ delta hel, & Wrn-/- MEFs. Topoisomerase I & beta -tubulin are nuclear & cytoplasmic markers, respectively. As expected the molecular weight of the Wrn delta hel mutant protein was lower than the Wrn WT protein. No band was detected in Wrn-/- MEFs fractions with the anti-Wrn polyclonal antibody. (B) Example of western blots showing only the presence of the Wrn delta hel mutant protein in total ER & the peroxisomal fractions. Fractionations were performed on WT, Wrn delta hel/ delta hel, & Wrn-/- liver tissues. Catalase & calreticulin are used as peroxisomal & ER specific markers. This experiment was repeated three times. (C) Protein levels of GRP78, phosphorylated IRE alpha , & beta -actin (top panels) & of total IRE alpha , & beta -actin (bottom panels) in the spleen of three animals of each genotype. (D) Ratio of GRP78 signal over beta -actin signal from western blots. (E) Ratio of IRE alpha over beta -actin signal from western blots. (F) Ratio of phosphorylated IRE alpha signal over beta -actin signal from western blots. Experiments were performed in triplicates. Bars in all histograms represent SEM. Tukey post ANOVA test P-values are shown (*P < 0.05 & **P < 0.01). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26447695), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - IER3IP1 suppression decreases the UPR activation(A) mRNA levels of IRE1 alpha , Perk & Atf6 in IER3IP1 KD cells. 4 days after infection with IER3IP1 shRNA lentivirus, mRNA levels of IRE1 alpha , Perk & Atf6 in IER3IP1 KD cells were determined by qRT-PCR. *P<0.05, **P<0.01 compared to control group. Values are mean ± SEM. (B) Effect of IER3IP1 suppression on the IRE1 alpha arm of the UPR. 4 days after IER3IP1 KD in MIN6 cells, phosphorylation of IRE1 alpha , Bip, sXBP-1 & uXBP-were determined by Western blot. The loaded proteins in the two “- or +” lines were the same but from the different plates. (C) mRNA levels of downstream targets of sXBP-1 in IER3IP1 KD cells. 4 days after IER3IP1 KD in MIN6 cells, mRNA levels of downstream targets of sXBP-1, Erdj4, p58IPK, EDEM & Sec61a, were determined by qRT-PCR. Values are mean ± SEM. (D) Effect of IER3IP1 suppression on the Perk arm of the UPR. 4 days after IER3IP1 KD in MIN6 cells, phosphorylation of Perk & eIF2a, & ATF4 were determined by Western blot. The loaded proteins in the two “- or +” lines were the same but from the different plates. (E) IER3IP1 KD decreases UPR activation induced by ER stress in MIN6. 4 days after IER3IP1 KD, MIN6 cells were treated with 10 μg/ml tunicamycin (TC) or 1 μM thapsigargin (TG) for 6 hours. Then the expression levels of Chop, sXBP-1, uXBP-1 & IER3IP1 were determined by Western blot. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28915629), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2323_rabbit-polyclonal-ire1-alpha-p-ser724-antibody-310202415525219.jpg "Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - IER3IP1 suppression decreases the UPR activation(A) mRNA levels of IRE1 alpha , Perk & Atf6 in IER3IP1 KD cells. 4 days after infection with IER3IP1 shRNA lentivirus, mRNA levels of IRE1 alpha , Perk & Atf6 in IER3IP1 KD cells were determined by qRT-PCR. *P<0.05, **P<0.01 compared to control group. Values are mean ± SEM. (B) Effect of IER3IP1 suppression on the IRE1 alpha arm of the UPR. 4 days after IER3IP1 KD in MIN6 cells, phosphorylation of IRE1 alpha , Bip, sXBP-1 & uXBP-were determined by Western blot. The loaded proteins in the two “- or +” lines were the same but from the different plates. (C) mRNA levels of downstream targets of sXBP-1 in IER3IP1 KD cells. 4 days after IER3IP1 KD in MIN6 cells, mRNA levels of downstream targets of sXBP-1, Erdj4, p58IPK, EDEM & Sec61a, were determined by qRT-PCR. Values are mean ± SEM. (D) Effect of IER3IP1 suppression on the Perk arm of the UPR. 4 days after IER3IP1 KD in MIN6 cells, phosphorylation of Perk & eIF2a, & ATF4 were determined by Western blot. The loaded proteins in the two “- or +” lines were the same but from the different plates. (E) IER3IP1 KD decreases UPR activation induced by ER stress in MIN6. 4 days after IER3IP1 KD, MIN6 cells were treated with 10 μg/ml tunicamycin (TC) or 1 μM thapsigargin (TG) for 6 hours. Then the expression levels of Chop, sXBP-1, uXBP-1 & IER3IP1 were determined by Western blot. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28915629), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - ATG5 & ATG7 influenced on autophagy, apoptosis & cell cycle through PERK/Nrf2 signaling. a The ER stress proteins were analysed by western blotting in Ad-GFP, Ad-ATG5 + Ad-ATG7,Ad-ATG5 + Ad-ATG7 + siPERK,Ad-ATG5 + Ad-ATG7 + siNrf2 & Ad-ATG5+ Ad-ATG7 + siPERK+siNrf2 induced chondrocytes. b The levels of ER stress proteins were normalized to beta -actin. c Determination of autophagy & apoptosis proteins expression by western blotting in Ad-GFP, Ad-ATG5 + Ad-ATG7, Ad-ATG5 + Ad-ATG7 + siPERK,Ad-ATG5 + Ad-ATG7 + siNrf2 & Ad-ATG5+ Ad-ATG7 + siPERK+ siNrf2 induced chondrocytes. dThe levels of related proteins were normalized to beta -actin. e FCM analysis was used to calculate the percentage of apoptotic cells at the time point of 24 h. The apoptosis rate were increased when combined treatment with silencing of PERK or Nrf2. Experiments were repeated 3 times, Representative images are shown. f Analysis of cell apoptosis. Data come from 3 independent experiments. g FCM analysis indicated that the S phase percentage were decreased compared to that of the control groups when combine infected with silencing of PERK or Nrf2 in C28I2 cells. Experiments were repeated 3 times, Representative images are shown. h Percentage of cells at each phase in different groups. *P < 0.05, **P < 0.01 compared with the controls. Values are means ± SD n = 3). (1:Ad-GFP, 2:Ad-ATG5 + Ad-ATG7, 3:Ad-ATG5 + Ad-ATG7 + siPERK, 4:Ad-ATG5 + Ad-ATG7 + siNrf2, 5:Ad-ATG5 + Ad-ATG7 + siPERK+ siNrf2) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31060556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2323_rabbit-polyclonal-ire1-alpha-p-ser724-antibody-31020241553727.jpg "Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - ATG5 & ATG7 influenced on autophagy, apoptosis & cell cycle through PERK/Nrf2 signaling. a The ER stress proteins were analysed by western blotting in Ad-GFP, Ad-ATG5 + Ad-ATG7,Ad-ATG5 + Ad-ATG7 + siPERK,Ad-ATG5 + Ad-ATG7 + siNrf2 & Ad-ATG5+ Ad-ATG7 + siPERK+siNrf2 induced chondrocytes. b The levels of ER stress proteins were normalized to beta -actin. c Determination of autophagy & apoptosis proteins expression by western blotting in Ad-GFP, Ad-ATG5 + Ad-ATG7, Ad-ATG5 + Ad-ATG7 + siPERK,Ad-ATG5 + Ad-ATG7 + siNrf2 & Ad-ATG5+ Ad-ATG7 + siPERK+ siNrf2 induced chondrocytes. dThe levels of related proteins were normalized to beta -actin. e FCM analysis was used to calculate the percentage of apoptotic cells at the time point of 24 h. The apoptosis rate were increased when combined treatment with silencing of PERK or Nrf2. Experiments were repeated 3 times, Representative images are shown. f Analysis of cell apoptosis. Data come from 3 independent experiments. g FCM analysis indicated that the S phase percentage were decreased compared to that of the control groups when combine infected with silencing of PERK or Nrf2 in C28I2 cells. Experiments were repeated 3 times, Representative images are shown. h Percentage of cells at each phase in different groups. *P < 0.05, **P < 0.01 compared with the controls. Values are means ± SD n = 3). (1:Ad-GFP, 2:Ad-ATG5 + Ad-ATG7, 3:Ad-ATG5 + Ad-ATG7 + siPERK, 4:Ad-ATG5 + Ad-ATG7 + siNrf2, 5:Ad-ATG5 + Ad-ATG7 + siPERK+ siNrf2) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31060556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - ER stress is induced by OGR1 via IRE1 alpha /JNK signalling. (A) Caco-2 cells were subjected to different pH medium, with or without an OGR1 inhibitor (10 µM), following 4–6 h in pH 7.6 serum free medium. After 24 h pH shift, total protein was isolated & Western blot performed. Results are representative of two independent experiments. (B) Caco-2 cells were subjected to different pH medium After 24 h pH shift, total protein was isolated & Western blot performed. Results are representative of two independent experiments. (C) Caco-2 cells were subjected to different pH medium with or without a JNK inhibitor (10 µM) following 4–6 h in pH 7.6 serum free medium. After 24 h pH shift, total protein was isolated & Western blot performed. Results are representative of two independent experiments. (D) Caco-2 cells were starved & subjected to an acidic pH with or without a JNK inhibitor as described in (C). After 24 h pH shift, total RNA was isolated & mRNA expression was investigated by qPCR. Statistical analysis was performed using one-way ANOVA followed by Tukey’s post-test. Data are presented as means ± SE of three independent experiments (***p < 0.001). (E) Caco-2 cells were starved & subjected to different pH medium following 4–6 h in pH 7.6 serum free medium. After 24 h pH shift, total protein was isolated & co-IP using IRE1 alpha antibody & JNK antibody was performed, followed by immunoblotting. Results are representative of two independent experiments. pH conditions: High pH 7.5–7.8; Normal pH 7.2–7.4; Low pH 6.6–6.8. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31996710), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2323_rabbit-polyclonal-ire1-alpha-p-ser724-antibody-310202415541960.jpg "Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - ER stress is induced by OGR1 via IRE1 alpha /JNK signalling. (A) Caco-2 cells were subjected to different pH medium, with or without an OGR1 inhibitor (10 µM), following 4–6 h in pH 7.6 serum free medium. After 24 h pH shift, total protein was isolated & Western blot performed. Results are representative of two independent experiments. (B) Caco-2 cells were subjected to different pH medium After 24 h pH shift, total protein was isolated & Western blot performed. Results are representative of two independent experiments. (C) Caco-2 cells were subjected to different pH medium with or without a JNK inhibitor (10 µM) following 4–6 h in pH 7.6 serum free medium. After 24 h pH shift, total protein was isolated & Western blot performed. Results are representative of two independent experiments. (D) Caco-2 cells were starved & subjected to an acidic pH with or without a JNK inhibitor as described in (C). After 24 h pH shift, total RNA was isolated & mRNA expression was investigated by qPCR. Statistical analysis was performed using one-way ANOVA followed by Tukey’s post-test. Data are presented as means ± SE of three independent experiments (***p < 0.001). (E) Caco-2 cells were starved & subjected to different pH medium following 4–6 h in pH 7.6 serum free medium. After 24 h pH shift, total protein was isolated & co-IP using IRE1 alpha antibody & JNK antibody was performed, followed by immunoblotting. Results are representative of two independent experiments. pH conditions: High pH 7.5–7.8; Normal pH 7.2–7.4; Low pH 6.6–6.8. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31996710), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - Thapsigargin treatment induces calpain activation & autophagy. (A) Control & shCAPNS1 cells were treated with 100 nM thapsigargin for 10, 20 & 30 min & the lysates subjected to western blot analysis to quantify the precursor & active form of CAPN1. The ratio between active CAPN1 & tubulin is reported below each lane. (B) Time course measurement of intracellular calcium concentration. The plot indicates the ratio of mean Indo Violet/Indo Blue emission values of each cell population at individual time points (minutes). The ratio corresponds to the relative calcium concentration of control & shCAPNS1 U2OS cells before & after addition of thapsigargin (100 nm final). Moving average was used as smoothing method. (C) shCAPNS1 cells were treated with 100 nM thapsigargin for 30, 60 & 120 min & the lysates subjected to western blot analysis to detect LC3, p62, pIRE, CAPNS1 & tubulin. (D) Levels of p62 & LC3 normalized to tubulin levels. Image collected & cropped by CiteAb from the following publication (//journals.biologists.com/bio/article/doi/10.1242/bio.022806/256614/Calpain-mobilizes-Atg9-Bif-1-vesicles-from-Golgi), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2323_rabbit-polyclonal-ire1-alpha-p-ser724-antibody-31020241553725.jpg "Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - Thapsigargin treatment induces calpain activation & autophagy. (A) Control & shCAPNS1 cells were treated with 100 nM thapsigargin for 10, 20 & 30 min & the lysates subjected to western blot analysis to quantify the precursor & active form of CAPN1. The ratio between active CAPN1 & tubulin is reported below each lane. (B) Time course measurement of intracellular calcium concentration. The plot indicates the ratio of mean Indo Violet/Indo Blue emission values of each cell population at individual time points (minutes). The ratio corresponds to the relative calcium concentration of control & shCAPNS1 U2OS cells before & after addition of thapsigargin (100 nm final). Moving average was used as smoothing method. (C) shCAPNS1 cells were treated with 100 nM thapsigargin for 30, 60 & 120 min & the lysates subjected to western blot analysis to detect LC3, p62, pIRE, CAPNS1 & tubulin. (D) Levels of p62 & LC3 normalized to tubulin levels. Image collected & cropped by CiteAb from the following publication (//journals.biologists.com/bio/article/doi/10.1242/bio.022806/256614/Calpain-mobilizes-Atg9-Bif-1-vesicles-from-Golgi), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - Abnormal Paneth cells show ER stress.(A, B) Representative transmission electron microscopy images of Paneth cells at the base of ileal crypts in (A) ICR & (B) SAMP1/YitFc mice. Scale bars indicate 2 μm. (C, D) Quantitative analysis of (C) granule number & (D) ER lumen diameter in Paneth cells (n = 3/each week for SAMP1/YitFc mice). For the measurements, three Paneth cells were randomly selected from each mouse. (E) SDS–PAGE Western blot analysis of ER stress markers, pIRE1 alpha , ATF4, cleaved-ATF6, & GRP78 in ileal crypts (n = 4/each group). Total-IRE1 alpha & HPRT1 was used as loading control. (F) Relative expression level of ER stress markers calculated from the band intensity. Error bars represent mean ± SEM. (C, D, F) Statistical significance was evaluated by t test in (C, D), & one-way ANOVA followed by Tukey’s post hoc test in (F). P < 0.05 was considered statistically significant. *P < 0.05, †P < 0.01, §P < 0.001. E, ER; G, granules; N, nucleus; n.s., not significant. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32345659), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2323_rabbit-polyclonal-ire1-alpha-p-ser724-antibody-31020241554530.jpg "Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - Abnormal Paneth cells show ER stress.(A, B) Representative transmission electron microscopy images of Paneth cells at the base of ileal crypts in (A) ICR & (B) SAMP1/YitFc mice. Scale bars indicate 2 μm. (C, D) Quantitative analysis of (C) granule number & (D) ER lumen diameter in Paneth cells (n = 3/each week for SAMP1/YitFc mice). For the measurements, three Paneth cells were randomly selected from each mouse. (E) SDS–PAGE Western blot analysis of ER stress markers, pIRE1 alpha , ATF4, cleaved-ATF6, & GRP78 in ileal crypts (n = 4/each group). Total-IRE1 alpha & HPRT1 was used as loading control. (F) Relative expression level of ER stress markers calculated from the band intensity. Error bars represent mean ± SEM. (C, D, F) Statistical significance was evaluated by t test in (C, D), & one-way ANOVA followed by Tukey’s post hoc test in (F). P < 0.05 was considered statistically significant. *P < 0.05, †P < 0.01, §P < 0.001. E, ER; G, granules; N, nucleus; n.s., not significant. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32345659), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - Hypoxia-induced downregulation of gamma -taxilin & alpha NAC & the subsequent ER responses are GSK-3 beta -dependent. (a) GSK-3 beta activation in hypoxia. Western blot analysis shows downregulation of phosphorylated (Ser 9) GSK-3 beta (phospho-GSK-3 beta ) in hypoxic (0–48 h) SK-N-SH & hypoxic (0–24 h) HeLa S3 cells. (b) Inhibition of GSK-3 beta activation by lithium chloride (LiCl, 100 mM) or CHIR-99021 (CHIR, 15 or 30 μM) maintained gamma -taxilin & alpha NAC protein levels in hypoxic SK-N-SH & HeLa S3 cells. (c) GSK-3 beta inhibition suppresses the expression of ER stress response proteins that are induced in hypoxic SK-N-SH cells. (d) GSK-3 beta RNA interference almost completely blocked the protein expression & restored the expression of gamma -taxilin & alpha NAC proteins in hypoxic HeLa S3 cells Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25880086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2323_rabbit-polyclonal-ire1-alpha-p-ser724-antibody-3102024155527.jpg "Western Blot: IRE1 alpha [p Ser724] Antibody - BSA Free [NB100-2323] - Hypoxia-induced downregulation of gamma -taxilin & alpha NAC & the subsequent ER responses are GSK-3 beta -dependent. (a) GSK-3 beta activation in hypoxia. Western blot analysis shows downregulation of phosphorylated (Ser 9) GSK-3 beta (phospho-GSK-3 beta ) in hypoxic (0–48 h) SK-N-SH & hypoxic (0–24 h) HeLa S3 cells. (b) Inhibition of GSK-3 beta activation by lithium chloride (LiCl, 100 mM) or CHIR-99021 (CHIR, 15 or 30 μM) maintained gamma -taxilin & alpha NAC protein levels in hypoxic SK-N-SH & HeLa S3 cells. (c) GSK-3 beta inhibition suppresses the expression of ER stress response proteins that are induced in hypoxic SK-N-SH cells. (d) GSK-3 beta RNA interference almost completely blocked the protein expression & restored the expression of gamma -taxilin & alpha NAC proteins in hypoxic HeLa S3 cells Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25880086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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![Western Blot: IRE1 alpha Antibody [NB100-2324] - ALOX15 knockout blocks alcohol-induced up-regulation of IRE1a in mouse liver. Image from verified customer review.](https://images.novusbio.com/images/IRE1-alpha-Antibody-Western-Blot-NB100-2324-img0009.jpg "Western Blot: IRE1 alpha Antibody [NB100-2324] - ALOX15 knockout blocks alcohol-induced up-regulation of IRE1a in mouse liver. Image from verified customer review.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
