| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-40256SS | |||
| Azide and BSA Free | NBP2-80583 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-75478SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76675-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77251-0.025mg | |||
![Western Blot: ATF6 Antibody (70B1413.1) [NBP1-40256] - Hypoxia leads to EMT and ER-stress in CRC cells. B. C. Confluent growing SW480 (B) and HCT116 (C) cells were cultured under conditions of normoxia or hypoxia-like conditions (serum free; 100 uM CoCl2, 1-9 h. B-actin (B-act) as loading control. HIF1a was detectable after 3 h of CoCl2 incubation, the amount of the 50 kD-ATF6 fragment, was already enhanced after 1 h of addition of CoCl2. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0087386) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/antibody/ATF6-Antibody-70B1413.jpg "<b>Biological Strategies Validation. </b>Western Blot: ATF6 Antibody (70B1413.1) [NBP1-40256] - Hypoxia leads to EMT and ER-stress in CRC cells. B. C. Confluent growing SW480 (B) and HCT116 (C) cells were cultured under conditions of normoxia or hypoxia-like conditions (serum free; 100 uM CoCl2, 1-9 h. B-actin (B-act) as loading control. HIF1a was detectable after 3 h of CoCl2 incubation, the amount of the 50 kD-ATF6 fragment, was already enhanced after 1 h of addition of CoCl2. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0087386) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Western Blot: ATF6 Antibody (70B1413.1) [NBP1-40256] - Analysis of ATF6 in mouse liver tissue using 3 ug/ml of ATF6 antibody and 0.25 ug/ml of GAPDH antibody. Lane A contains 20 ugs of whole mouse liver lysate, lane B contains 20 ugs of total ER fraction, and lane C contains 20 ugs of rough ER fraction. The ATF6 band may represent under glycosylated or cleaved/active ATF6.](http://images.novusbio.com/fullsize/ATF6-Antibody-70B1413-1-Western-Blot-NBP1-40256-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: ATF6 Antibody (70B1413.1) [NBP1-40256] - Untreated HeLa cells were fixed in -20C methanol for 10 min, air dried and rehydrated in PBS at room temperature for 5 minutes. Cells were incubated with anti-ATF6 (1:20) for one hour at room temperature. ATF6 reactivity (green) was detected with anti-mouse Dylight-488 secondary antibody. Nuclei were counterstained with DAPI (blue). Note the ER localization of ATF6.](http://images.novusbio.com/fullsize/ATF6-Antibody-70B1413-1-Immunocytochemistry-Immunofluorescence-NBP1-40256-img0014.jpg)
![Immunohistochemistry-Paraffin: ATF6 Antibody (70B1413.1) [NBP1-40256] - Analysis of ATF6 antibody on Human placenta. Fixed paraffin-embedded sections. Antibody dilution 1:50. Incubated overnight in 4C. Image from verified customer review.](http://images.novusbio.com/fullsize/ATF6-Antibody-70B1413-1-Immunohistochemistry-Paraffin-NBP1-40256-img0017.jpg)
![Flow (Intracellular): ATF6 Antibody (70B1413.1) [NBP1-40256] - An intracellular stain was performed on HeLa cells with ATF6 Antibody (70B1413.1) NBP1-40256F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.. Using the FITC format of this antibody.](http://images.novusbio.com/fullsize/ATF6-Antibody-70B1413-1-Flow-Intracellular-NBP1-40256-img0016.jpg)
![Western Blot: ATF6 Antibody (70B1413.1) [NBP1-40256] - Lane 1: 293 cells transfected with full-length ATF6. Lane 2: 293 cells transfected with partial length ATF6 (amino acids 1-373). Lane 3: Untransfected 293 cells. Western blots were probed with 4 ug/ml of the ATF6 monoclonal antibody and visualized with PicoTect Western Blot Chemiluminescence Substrate (10087K). Film was exposed for 1 min. The top arrow corresponds to the 90 kDa form of ATF6 described as full-length in the literature. The human full-length and partial length ATF6 plasmids are described in Luo and Lee (2002).](http://images.novusbio.com/fullsize/ATF6-Antibody-70B1413-1-Western-Blot-NBP1-40256-img0007.jpg)
![Immunohistochemistry-Paraffin: ATF6 Antibody (70B1413.1) [NBP1-40256] - Human placenta, followed by biotinylated horse anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Dilution 10ug/ml](http://images.novusbio.com/fullsize/ATF6-Antibody-70B1413-1-Immunohistochemistry-Paraffin-NBP1-40256-img0001.jpg)
![Flow Cytometry: ATF6 Antibody (70B1413.1) [NBP1-40256] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using ATF6 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/ATF6-Antibody-70B1413-1-Flow-Cytometry-NBP1-40256-img0009.jpg)
![Western Blot: ATF6 Antibody (70B1413.1) [NBP1-40256] - Lysate of DU145 cells. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-40256_mouse-monoclonal-atf6-antibody-70b1413-1-imgenex-img-273-western-blot-672023151028..jpg)
![Western Blot: ATF6 Antibody (70B1413.1) [NBP1-40256] - Lysate of PC-3 cells. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-40256_mouse-monoclonal-atf6-antibody-70b1413-1-imgenex-img-273-western-blot-672023151236..jpg)
![Disturbance of ER homeostasis in ADTKD-UMOD. (D) Protein abundances of BiP, phospho-eIF2alpha, eIF2alpha, ATF4, both full-length (*) and cleaved activated (#) ATF6, and CHOP were increased in Umod mutant mice compared to wild-type mice. Signal intensities were corrected for GAPDH signal intensities of the same PVDF-membrane, which was stripped several times to facilitate the detection of multiple proteins. Mean of protein abundance of wild-type mice was set on a value of 1 [mean (wild-type) = 1]. Data are shown as means +/- SD. One-way ANOVA with Newman-Keuls's post hoc test: p vs. wild-type, *p < 0.05; **p < 0.01; ***p < 0.001. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep42970), licensed under a CC-BY licence.](http://images.novusbio.com/fullsize/nbp1-40256_mouse-monoclonal-atf6-antibody-70b1413-1-2912202311472192.jpg)
![Western Blot: ATF6 Antibody [NBP1-75478] - Total protein from HeLa cells treated with and without 1 mM DTT for 24 hours was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 1% BSA. The membrane was probed with 2.0 ug/ml anti-ATF6 in block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the decrease in full length ATF6 (90 kDa, arrow) and increase in cleaved ATF6 (55 kDa, arrowhead) upon DTT treatment and the activation of the unfolded protein response pathway.](http://images.novusbio.com/fullsize/ATF6-Antibody-Western-Blot-NBP1-75478-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: ATF6 Antibody [NBP1-75478] - Total protein from HeLa cells treated with and without 1 mM DTT for 24 hours was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 1% BSA. The membrane was probed with 2.0 ug/ml anti-ATF6 in block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the decrease in full length ATF6 (90 kDa, arrow) and increase in cleaved ATF6 (55 kDa, arrowhead) upon DTT treatment and the activation of the unfolded protein response pathway.")
![<b>Biological Strategies Validation. </b>Simple Western: ATF6 Antibody [NBP1-75478] - Simple Western lane view shows a specific band for ATF6 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.](http://images.novusbio.com/fullsize/ATF6-Antibody-Simple-Western-NBP1-75478-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: ATF6 Antibody [NBP1-75478] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-ATF6 at 10 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/ATF6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-75478-img0005.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: ATF6 Antibody - BSA Free [NBP1-75478] - AICAR inhibits ER stress responses. Western analysis of cell lysates of nuclear extracts. AMPK-independent inhibition of human macrophage ER stress response by AICAR. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27562249/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ATF6-Antibody-Western-Blot-NBP1-75478-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: ATF6 Antibody [NBP1-75478] - Antibody was tested in HeLa cells with FITC (green). Nuclei were counterstained with Dapi (blue).](http://images.novusbio.com/fullsize/ATF6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-75478-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: ATF6 Antibody [NBP1-76675] - Staining of ATF6 in human pancreas tissue with ATF6 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/ATF6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76675-img0012.jpg "Immunocytochemistry/Immunofluorescence: ATF6 Antibody [NBP1-76675] - Staining of ATF6 in human pancreas tissue with ATF6 antibody at 20 ug/ml.")
![Western Blot: ATF6 Antibody [NBP1-76675] - Analysis in EL4 cell lysate with antibody at (A) 0.5 and (B) 1 ug/mL.](http://images.novusbio.com/fullsize/ATF6-Antibody-Western-Blot-NBP1-76675-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: ATF6 Antibody [NBP1-76675] - MCF7 cells using ATF6 antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/ATF6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76675-img0002.jpg)
![Immunohistochemistry: ATF6 Antibody [NBP1-76675] - Staining of ATF6 in human breast tissue with ATF6 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/ATF6-Antibody-Immunohistochemistry-NBP1-76675-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: ATF6 Antibody [NBP1-76675] - Staining of ATF6 in human breast tissue with ATF6 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/ATF6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76675-img0011.jpg)
![Immunohistochemistry: ATF6 Antibody [NBP1-76675] - Staining of ATF6 in human pancreas tissue with ATF6 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/ATF6-Antibody-Immunohistochemistry-NBP1-76675-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: ATF6 Antibody [NBP1-76675] - Immunofluorescence of ATF6 in MCF7 cells with ATF6 antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/ATF6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76675-img0004.jpg)
![Western Blot: ATF6 Antibody [NBP1-77251] - Analysis in MCF7 cell lysate with antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/ATF6-Antibody-Western-Blot-NBP1-77251-img0004.jpg "Western Blot: ATF6 Antibody [NBP1-77251] - Analysis in MCF7 cell lysate with antibody at (A) 1 and (B) 2 ug/mL.")
![Immunocytochemistry/Immunofluorescence: ATF6 Antibody [NBP1-77251] - MCF7 cells at 20 ug/ml.](http://images.novusbio.com/fullsize/ATF6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77251-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: ATF6 Antibody [NBP1-77251] - A20 with ATF6 antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/ATF6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77251-img0002.jpg)
![Immunohistochemistry-Paraffin: ATF6 Antibody [NBP2-48501] - Staining of human testis shows moderate cytoplasmic positivity in a subset of cells in seminiferous ducts.](http://images.novusbio.com/fullsize/ATF6-Antibody-Immunohistochemistry-Paraffin-NBP2-48501-img0008.jpg "Immunohistochemistry-Paraffin: ATF6 Antibody [NBP2-48501] - Staining of human testis shows moderate cytoplasmic positivity in a subset of cells in seminiferous ducts.")
![Immunohistochemistry-Paraffin: ATF6 Antibody [NBP2-48501] - Staining of human kidney shows weak to moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/ATF6-Antibody-Immunohistochemistry-Paraffin-NBP2-48501-img0005.jpg)
![Immunohistochemistry-Paraffin: ATF6 Antibody [NBP2-48501] - Staining of human prostate shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ATF6-Antibody-Immunohistochemistry-Paraffin-NBP2-48501-img0006.jpg)
![Immunohistochemistry-Paraffin: ATF6 Antibody [NBP2-48501] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/ATF6-Antibody-Immunohistochemistry-Paraffin-NBP2-48501-img0007.jpg)
![Western Blot: ATF6 Antibody (S05-4E7) [NBP3-14903] - Western blot detection of ATF6 in A549, HL-60, U2OS cell lysates using ATF6 Rabbit mAb (1:1000 diluted). Predicted band size: 75kDa. Observed band size: 90-100kDa.](http://images.novusbio.com/fullsize/ATF6-Antibody-S05-4E7-Western-Blot-NBP3-14903-img0001.jpg "Western Blot: ATF6 Antibody (S05-4E7) [NBP3-14903] - Western blot detection of ATF6 in A549, HL-60, U2OS cell lysates using ATF6 Rabbit mAb (1:1000 diluted). Predicted band size: 75kDa. Observed band size: 90-100kDa.")
![Immunohistochemistry: ATF6 Antibody (S05-4E7) [NBP3-14903] - Immunohistochemical of ATF6 in Human lung cancer tissue using ATF6 antibody at dilution 1/20](http://images.novusbio.com/fullsize/ATF6-Antibody-S05-4E7-Immunohistochemistry-NBP3-14903-img0002.jpg)
![ELISA: Human ATF6 ELISA Kit (Colorimetric) [NBP2-69885] - Samples were spiked with high concentrations of Human ATF6 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-69885_human-atf6-elisa-kit-colorimetric-132202416143096.jpg "ELISA: Human ATF6 ELISA Kit (Colorimetric) [NBP2-69885] - Samples were spiked with high concentrations of Human ATF6 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human ATF6 ELISA Kit (Colorimetric) [NBP2-69885] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-69885_human-atf6-elisa-kit-colorimetric-43202495066.jpg)
![ELISA: Mouse ATF6 ELISA Kit (Colorimetric) [NBP2-69886] - Samples were spiked with high concentrations of Mouse ATF6 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-69886_mouse-atf6-elisa-kit-colorimetric-132202416102294.jpg "ELISA: Mouse ATF6 ELISA Kit (Colorimetric) [NBP2-69886] - Samples were spiked with high concentrations of Mouse ATF6 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse ATF6 ELISA Kit (Colorimetric) [NBP2-69886] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-69886_mouse-atf6-elisa-kit-colorimetric-4320249124835.jpg)
![SDS-Page: Recombinant Human ATF6 Protein [H00022926-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-ATF6-Protein-SDS-Page-H00022926-P01-img0002.jpg "SDS-Page: Recombinant Human ATF6 Protein [H00022926-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![ELISA: Rat ATF6 ELISA Kit (Colorimetric) [NBP2-69887] - Samples were spiked with high concentrations of Rat ATF6 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-69887_rat-atf6-elisa-kit-colorimetric-13220241620457.jpg "ELISA: Rat ATF6 ELISA Kit (Colorimetric) [NBP2-69887] - Samples were spiked with high concentrations of Rat ATF6 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat ATF6 ELISA Kit (Colorimetric) [NBP2-69887] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-69887_rat-atf6-elisa-kit-colorimetric-432024973670.jpg)