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ATF6 Antibody - BSA Free

Images

 
Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-ATF6 antibody at 5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat ...read more
Loading: 15 ug of rat kidney lysate Antibodies: ATF6, 1 ug/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Loading: 15 ug of mouse tissue lysate Antibodies: ATF6, 1 ug/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ATF6 antibody at 5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat ...read more
Genetic Strategies: Loading: 15 ug of human HeLa cell lysate Antibodies ATF6, 0.5 ug/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Loading: 15 ug of human cell lysate Antibodies ATF6, 1 ug/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.Secondary: Goat Anti-Rabbit IgG HRP conjugate at ...read more
Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-ATF6 antibody at 5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

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ATF6 Antibody - BSA Free Summary

Immunogen
Antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human ATF6. The immunogen is located within amino acids 30 - 80 of ATF6. Amino Acid Squence: ELQLEAANETYENNFDN
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATF6
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry 5 ug/mL
  • Immunohistochemistry-Paraffin
  • Western Blot 0.5-1 ug/ml
Application Notes
Use in IHC-P reported in scientific literature (PMID:33603856).
Control
HeLa DTT Treated / Untreated Cell Lysate
Control Peptide
ATF6 Protein (NBP1-77251PEP)
Publications
Read Publications using
NBP1-77251 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for ATF6 Antibody - BSA Free

  • ACHM7
  • Activating transcription factor 6 alpha
  • activating transcription factor 6
  • atf6 a
  • ATF6 alpha
  • ATF6
  • ATF6A
  • ATF6-alpha
  • cAMP-dependent transcription factor ATF-6 alpha
  • cyclic AMP-dependent transcription factor ATF-6 alpha

Background

Disruptions of protein folding and maturation in the endoplasmic reticulum (ER) result in the activation of the unfolded protein response (UPR), an integrated cellular signaling pathway that transmits information from the ER lumen to the cytoplasm and nucleus. Activating transcription factor 6 (ATF6) as well as the ER-transmembrane protein kinases IRE1p and PERK are the major transducers of the UPR . ATF6 is an ER transmembrane protein that is normally bound to the ER chaperone GRP78, but upon ER stress is released from GRP78 and proteolytically cleaved to yield a cytosolic fragment which then migrates to the nucleus, and together with the transcription factor XBP-1, activates transcription of UPR-responsive genes. ATF6 has two isoforms (ATF6alpha and ATF6beta); only ATF6alpha is recognized by this antibody.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ATF6 Antibody (NBP1-77251)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC-P.


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IHC-P
(1)
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Mouse
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ATF6 Antibody (NBP1-77251). (Showing 1 - 2 of 2 FAQs).

  1. We are looking for an antibody specific to ATF6 alpha that will not cross-react with ATF6 beta. Would you please help confirm if any of the following are ATF6 alpha specific antibodies: NBP1-40256, NBP1-77251, NBP1-76675 & NBP1-41439?
    • The antibodies NBP1-77251 and NBP1-76675 are specific to ATF6 alpha and should not cross-react with ATF6 beta.
  2. May we ask if ATF6 is ATF6 alpha? We are looking for ATF6 alpha antibody for our customer, but found none from Novus. Only ATF6 antibodies. However, after checking http://www.uniprot.org/uniprot/P18850, I found ATF6 and ATF6 alpha seem identical. Would you please help confirm if NBP1-40256, NBP1-77251, NBP1-76675 & NBP1-41439 are all ATF6 alpha antibodies?
    • The antibodies NBP1-77251, NBP1-76675 are specific to ATF6A and should not cross-react with ATF6B.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional ATF6 Products

Research Areas for ATF6 Antibody (NBP1-77251)

Find related products by research area.

Blogs on ATF6.

ATF6 - monitoring and regulating protein folding under cellular stress
During times of cellular stress overloading of the protein folding machinery leads to the accumulation of incorrectly folded proteins. This triggers the unfolded protein response (UPR) in order to try to reestablish homeostasis or, if this fails, t...  Read full blog post.

ATF6 - a key target in alcohol-induced fatty liver disease?
The ATF6 endoplasmic reticulum (ER) stress-regulated transcription factor is constitutively expressed and plays a central role in the mammalian unfolded protein response (UPR). This fundamental pathway is responsible for balancing cellular hom...  Read full blog post.

A Key to Fight Stress: ATF6
The protein ATF6 is a constitutively expressed transcription factor that is a key mediator of the unfolded protein response (UPR) that allows mammalian cells to maintain cellular homeostasis under conditions of environmental and physiological stress. ...  Read full blog post.

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Bioinformatics

Gene Symbol ATF6
Uniprot