![ELISA: Rat ATF6 ELISA Kit (Colorimetric) [NBP2-69887] - Samples were spiked with high concentrations of Rat ATF6 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-69887_rat-atf6-elisa-kit-colorimetric-13220241620457.jpg "ELISA: Rat ATF6 ELISA Kit (Colorimetric) [NBP2-69887] - Samples were spiked with high concentrations of Rat ATF6 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
| Reactivity | RtSpecies Glossary |
| Applications | ELISA |
| Suitable Sample Type | Serum, plasma and other biological fluids |
| Standard Curve Range | 0.63 - 40 ng/mL |
| Sensitivity | 0.38 ng/mL |
| Specificity | This kit recognizes Rat ATF6 in samples. No significant cross-reactivity or interference between Rat ATF6 and analogues was observed. |
| Standard Curve Range | 0.63 - 40 ng/mL |
| Sensitivity | 0.38 ng/mL |
| Assay Type | Sandwich-ELISA |
| Inter-Assay | CV% < 5.93% |
| Intra-Assay | CV% < 4.72% |
| Spike Recovery | 85-104% |
| Sample Volume | 100 uL |
| Kit Type | ELISA Kit (Colorimetric) |
| Gene | ATF6 |
| Storage | Storage of components varies. See protocol for specific instructions. |
| Components |
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![Immunohistochemistry-Paraffin IRE1 alpha [p Ser724] Antibody - BSA Free](https://images.novusbio.com/images/IRE1-alpha-[p-Ser724]-Antibody-Immunohistochemistry-Paraffin-NB100-2323-img0025.jpg)
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Research Areas for ATF6 ELISA Kit (NBP2-69887)Find related products by research area.
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ATF6 - monitoring and regulating protein folding under cellular stress During times of cellular stress overloading of the protein folding machinery leads to the accumulation of incorrectly folded proteins. This triggers the unfolded protein response (UPR) in order to try to reestablish homeostasis or, if this fails, t... Read full blog post. |
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ATF6 - a key target in alcohol-induced fatty liver disease? The ATF6 endoplasmic reticulum (ER) stress-regulated transcription factor is constitutively expressed and plays a central role in the mammalian unfolded protein response (UPR). This fundamental pathway is responsible for balancing cellular hom... Read full blog post. |
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A Key to Fight Stress: ATF6 The protein ATF6 is a constitutively expressed transcription factor that is a key mediator of the unfolded protein response (UPR) that allows mammalian cells to maintain cellular homeostasis under conditions of environmental and physiological stress. ... Read full blog post. |
| Gene Symbol | ATF6 |
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![Western Blot: ATF6 Antibody (70B1413.1) [NBP1-40256] - Hypoxia leads to EMT and ER-stress in CRC cells. B. C. Confluent growing SW480 (B) and HCT116 (C) cells were cultured under conditions of normoxia or hypoxia-like conditions (serum free; 100 uM CoCl2, 1-9 h. B-actin (B-act) as loading control. HIF1a was detectable after 3 h of CoCl2 incubation, the amount of the 50 kD-ATF6 fragment, was already enhanced after 1 h of addition of CoCl2. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0087386) licensed under a CC-BY license.](https://images.novusbio.com/images/antibody/ATF6-Antibody-70B1413.jpg "Western Blot: ATF6 Antibody (70B1413.1) [NBP1-40256] - Hypoxia leads to EMT and ER-stress in CRC cells. B. C. Confluent growing SW480 (B) and HCT116 (C) cells were cultured under conditions of normoxia or hypoxia-like conditions (serum free; 100 uM CoCl2, 1-9 h. B-actin (B-act) as loading control. HIF1a was detectable after 3 h of CoCl2 incubation, the amount of the 50 kD-ATF6 fragment, was already enhanced after 1 h of addition of CoCl2. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0087386) licensed under a CC-BY license.")