![Immunocytochemistry/Immunofluorescence: AMPK alpha 1/2 Antibody [NBP3-05628] - Immunofluorescence analysis of U2OS cells using AMPK alpha 1/2 Rabbit pAb (NBP3-05628) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/AMPK-alpha-1-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05628-img0003.jpg "Immunocytochemistry/Immunofluorescence: AMPK alpha 1/2 Antibody [NBP3-05628] - Immunofluorescence analysis of U2OS cells using AMPK alpha 1/2 Rabbit pAb (NBP3-05628) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 13-300 of human PRKAA1/PRKAA2 (NP_006242.5). AEKQKHDGRVKIGHYILGDTLGVGTFGKVKVGKHELTGHKVAVKILNRQKIRSLDVVGKIRREIQNLKLFRHPHIIKLYQVISTPSDIFMVMEYVSGGELFDYICKNGRLDEKESRRLFQQILSGVDYCHRHMVVHRDLKPENVLLDAHMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSSGVILYALLCGTLPFDDDHVPTLFKKICDGIFYTPQYLNPSVISLLKHMLQVDPMKRATIKDIREHEWFKQDLPKYLFPEDPSYSSTMID |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PRKAA1 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS with 50% glycerol, pH7.3. |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
![Western Blot AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/Akt3_AF887_Western_Blot_5930.jpg)
![Simple Western AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/16332.jpg)
![Intracellular Staining by Flow Cytometry AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/Akt3_AF887_Flow_Cytometry_8283.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | PRKAA1 |
![Immunohistochemistry-Paraffin: AMPK alpha 1/2 Antibody [NBP3-05628] - Immunohistochemistry of paraffin-embedded rat lung using AMPK alpha 1/2 Rabbit pAb (NBP3-05628) at dilution of 1:25 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/AMPK-alpha-1-2-Antibody-Immunohistochemistry-Paraffin-NBP3-05628-img0006.jpg "Immunohistochemistry-Paraffin: AMPK alpha 1/2 Antibody [NBP3-05628] - Immunohistochemistry of paraffin-embedded rat lung using AMPK alpha 1/2 Rabbit pAb (NBP3-05628) at dilution of 1:25 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.")
![Immunocytochemistry/Immunofluorescence: AMPK alpha 1/2 Antibody [NBP3-05628] - Immunofluorescence analysis of NIH/3T3 cells using AMPK alpha 1/2 Rabbit pAb (NBP3-05628) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/AMPK-alpha-1-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05628-img0001.jpg "Immunocytochemistry/Immunofluorescence: AMPK alpha 1/2 Antibody [NBP3-05628] - Immunofluorescence analysis of NIH/3T3 cells using AMPK alpha 1/2 Rabbit pAb (NBP3-05628) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.")
![Immunocytochemistry/Immunofluorescence: AMPK alpha 1/2 Antibody [NBP3-05628] - Immunofluorescence analysis of PC-12 cells using AMPK alpha 1/2 Rabbit pAb (NBP3-05628) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/AMPK-alpha-1-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05628-img0002.jpg "Immunocytochemistry/Immunofluorescence: AMPK alpha 1/2 Antibody [NBP3-05628] - Immunofluorescence analysis of PC-12 cells using AMPK alpha 1/2 Rabbit pAb (NBP3-05628) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.")
![Immunohistochemistry-Paraffin: AMPK alpha 1/2 Antibody [NBP3-05628] - Immunohistochemistry of paraffin-embedded human colon using AMPK alpha 1/2 Rabbit pAb (NBP3-05628) at dilution of 1:25 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/AMPK-alpha-1-2-Antibody-Immunohistochemistry-Paraffin-NBP3-05628-img0004.jpg "Immunohistochemistry-Paraffin: AMPK alpha 1/2 Antibody [NBP3-05628] - Immunohistochemistry of paraffin-embedded human colon using AMPK alpha 1/2 Rabbit pAb (NBP3-05628) at dilution of 1:25 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.")
![Immunohistochemistry-Paraffin: AMPK alpha 1/2 Antibody [NBP3-05628] - Immunohistochemistry of paraffin-embedded mouse kidney using AMPK alpha 1/2 Rabbit pAb (NBP3-05628) at dilution of 1:25 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/AMPK-alpha-1-2-Antibody-Immunohistochemistry-Paraffin-NBP3-05628-img0005.jpg "Immunohistochemistry-Paraffin: AMPK alpha 1/2 Antibody [NBP3-05628] - Immunohistochemistry of paraffin-embedded mouse kidney using AMPK alpha 1/2 Rabbit pAb (NBP3-05628) at dilution of 1:25 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.")
![Western Blot: AMPK alpha 1/2 Antibody [NBP3-05628] - analysis of various lysates, using AMPKa1/AMPKa2 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/antibody/nbp3-05628_rabbit-polyclonal-ampk-alpha-1-2-antibody-western-blot-882023105441..jpg "Western Blot: AMPK alpha 1/2 Antibody [NBP3-05628] - analysis of various lysates, using AMPKa1/AMPKa2 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/Insulin_MAB1417_Immunocytochemistry_9376.jpg)
![Immunohistochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/mab1417_human-bovine-mouse-insulin-mab-clone-182410-immunohistochemistry-308202115145.jpg)
![Immunohistochemistry-Paraffin TOR/mTOR [p Ser2448] Antibody](https://images.novusbio.com/images/TOR-mTOR-[p-Ser2448]-Antibody-Immunohistochemistry-Paraffin-NB600-607-img0005.jpg)
![Western Blot TOR/mTOR [p Ser2448] Antibody](https://images.novusbio.com/images/TOR-mTOR-[p-Ser2448]-Antibody-Western-Blot-NB600-607-img0006.jpg)
![Data TOR/mTOR [p Ser2448] Antibody](https://images.novusbio.com/images/TOR-mTOR-[p-Ser2448]-Antibody-N-A-NB600-607-img0008.jpg)
![N/A Adiponectin/Acrp30 [HRP]](https://images.novusbio.com/images2/DATA_Adiponectin_DRP300_ELISA_812.jpg)
![N/A Adiponectin/Acrp30 [HRP]](https://images.novusbio.com/images2/DATA_Adiponectin_DRP300_ELISA_813.jpg)
![N/A Adiponectin/Acrp30 [HRP]](https://images.novusbio.com/images2/Adiponectin_DRP300_ELISA_173.jpg)
![Western Blot ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Western_Blot_5097.jpg)
![Immunohistochemistry ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Immunohistochemistry_20696.jpg)
![Knockout Validated ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Knockout_Validated_22864.jpg)
![N/A Leptin/OB [HRP]](https://images.novusbio.com/images2/Leptin_DLP00_ELISA_134.jpg)
![N/A Leptin/OB [HRP]](https://images.novusbio.com/images2/DATA_Leptin_DLP00_ELISA_749.jpg)
![Western Blot AMPK alpha 2 Antibody [Unconjugated]](https://images.novusbio.com/images2/AMPK_alpha_2_AF2850_Western_Blot_5219.jpg)
![Simple Western AMPK alpha 2 Antibody [Unconjugated]](https://images.novusbio.com/images2/AMPK_alpha_2_AF2850_Simple_Western_16678.jpg)
![Immunocytochemistry AMPK alpha 2 Antibody [Unconjugated]](https://images.novusbio.com/images2/AMPK_alpha_2_AF2850_Immunocytochemistry__Immunofluorescence_19686.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/DATA_IL6_M6000_ELISA_936.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/IL-6_M6000_ELISA_415.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images/m6000b_mouse-il-6-quantikine-elisa-kit-44202415433118.jpg)
![Simple Western PYK2/FAK2 [p Tyr402] Antibody (592918) [Unconjugated]](https://images.novusbio.com/images2/PYK2_MAB6210_Simple_Western_16639.jpg)
![Western Blot PYK2/FAK2 [p Tyr402] Antibody (592918) [Unconjugated]](https://images.novusbio.com/images2/PYK2_MAB6210_Western_Blot_9430.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")