![Western Blot: Alkaline Phosphatase/ALPP Antibody (ALPP/2899R) [NBP3-07800] - Western Blot Analysis of JEG-3 cell lysate using Alkaline Phosphatase/ALPP Rabbit Recombinant Monoclonal Antibody (ALPP/2899R).](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-ALPP-Antibody-ALPP-2899R-Western-Blot-NBP3-07800-img0002.jpg "Western Blot: Alkaline Phosphatase/ALPP Antibody (ALPP/2899R) [NBP3-07800] - Western Blot Analysis of JEG-3 cell lysate using Alkaline Phosphatase/ALPP Rabbit Recombinant Monoclonal Antibody (ALPP/2899R).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC |
| Clone | ALPP/2899R |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08817) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant full-length human Alkaline Phosphatase/ALPP protein (Uniprot: P05187) |
| Localization | Cytoplasmic and cell surface |
| Marker | Germ Cell Tumor Marker |
| Specificity | Reacts with a 70kDa membrane-bound isozyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP reacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic, and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | ALPP |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. No special pretreatment is required for the immunohistochemical staining of formalin-fixed tissues. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 70 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody (ALPP/2899R) [NBP3-07800] - Formalin-fixed, paraffin-embedded human placenta stained with Alkaline Phosphatase/ALPP Rabbit Recombinant Monoclonal Antibody (ALPP/2899R).](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-ALPP-Antibody-ALPP-2899R-Immunohistochemistry-Paraffin-NBP3-07800-img0001.jpg "Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody (ALPP/2899R) [NBP3-07800] - Formalin-fixed, paraffin-embedded human placenta stained with Alkaline Phosphatase/ALPP Rabbit Recombinant Monoclonal Antibody (ALPP/2899R).")
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