I am looking to use a placental alkaline phosphatase antibody for sorting syncytiotrophoblast material from female tissue. It looks like most people who use these antibodies use in-house versions and references for commercially available antibodies I've found are from the '80's. I noticed you have several different monoclonal antibodies and was hoping you could give me a little more information on them and potential applications they've been tested for. Also, do you have a biotinylated or fluorophore-conjugated version?

Name: Alkaline Phosphatase/ALPP Antibody (ALPP/2899R) [Janelia Fluor&#174; 646]
Brand: Novus Biologicals
SKU: NBP3-08817JF646
Price: 519 USD

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Accepted Answer

None of our placental alkaline phosphatase antibodies are biotinylated or fluorchrome conjugated but we offer a number of kits for this application and our lab can do a custom conjugation as well, for an additional fee. NB600-750 is useful in FACS with human cells, so I would recommend this antibody for your experiment. We back the use of this antibody as stated on the datasheet with the 100% Novus Guarantee. If you have questions about another antibody, I will be happy to answer them.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	WB, IHC
Clone	ALPP/2899R
Clonality	Monoclonal
Host	Rabbit
Conjugate	Janelia Fluor 646

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 NBP3-08817AF350

Alexa Fluor 405 NBP3-08817AF405

Alexa Fluor 488 NBP3-08817AF488

Alexa Fluor 532 NBP3-08817AF532

Alexa Fluor 594 NBP3-08817AF594

Alexa Fluor 647 NBP3-08817AF647

Alexa Fluor 700 NBP3-08817AF700

Alexa Fluor 750 NBP3-08817AF750

Allophycocyanin NBP3-08817APC

Biotin NBP3-08817B

CoraFluor 1 NBP3-08817CL1

DyLight 350 NBP3-08817UV

DyLight 405 NBP3-08817V

DyLight 488 NBP3-08817G

DyLight 550 NBP3-08817R

DyLight 594 NBP3-08817DL594

DyLight 650 NBP3-08817C

DyLight 680 NBP3-08817FR

DyLight 755 NBP3-08817IR

FITC NBP3-08817F

HRP NBP3-08817H

Janelia Fluor 525 NBP3-08817JF525

Janelia Fluor 549 NBP3-08817JF549

Janelia Fluor 585 NBP3-08817JF585

Janelia Fluor 635 NBP3-08817JF635

Janelia Fluor 669 NBP3-08817JF669

PE NBP3-08817PE

PerCP NBP3-08817PCP

Unconjugated NBP3-08817

Catalog# & Formulation	Size	Price
Standard Formulation NBP3-08817

Azide and BSA Free NBP3-08817

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-08817AF350
Alexa Fluor 405	NBP3-08817AF405
Alexa Fluor 488	NBP3-08817AF488
Alexa Fluor 532	NBP3-08817AF532
Alexa Fluor 594	NBP3-08817AF594
Alexa Fluor 647	NBP3-08817AF647
Alexa Fluor 700	NBP3-08817AF700
Alexa Fluor 750	NBP3-08817AF750
Allophycocyanin	NBP3-08817APC
Biotin	NBP3-08817B
CoraFluor 1	NBP3-08817CL1
DyLight 350	NBP3-08817UV
DyLight 405	NBP3-08817V
DyLight 488	NBP3-08817G
DyLight 550	NBP3-08817R
DyLight 594	NBP3-08817DL594
DyLight 650	NBP3-08817C
DyLight 680	NBP3-08817FR
DyLight 755	NBP3-08817IR
FITC	NBP3-08817F
HRP	NBP3-08817H
Janelia Fluor 525	NBP3-08817JF525
Janelia Fluor 549	NBP3-08817JF549
Janelia Fluor 585	NBP3-08817JF585
Janelia Fluor 635	NBP3-08817JF635
Janelia Fluor 669	NBP3-08817JF669
PE	NBP3-08817PE
PerCP	NBP3-08817PCP
Unconjugated	NBP3-08817

Alkaline Phosphatase/ALPP Antibody (ALPP/2899R) [Janelia Fluor® 646]

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Additional Information	Recombinant Monoclonal Antibody
Immunogen	Recombinant full-length human Alkaline Phosphatase/ALPP protein (Uniprot: P05187)
Localization	Cytoplasmic and cell surface
Marker	Germ Cell Tumor Marker
Specificity	Reacts with a 70kDa membrane-bound isozyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP reacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic, and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease.
Isotype	IgG
Clonality	Monoclonal
Host	Rabbit
Gene	ALPP
Purity	Protein A or G purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Dilutions	Immunohistochemistry-Paraffin Western Blot
Application Notes	Optimal dilution of this antibody should be experimentally determined.

Storage	Store at 4C in the dark.
Buffer	50mM Sodium Borate
Preservative	0.05% Sodium Azide
Purity	Protein A or G purified