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Alix Antibody (OTI1A4)

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Western Blot: Alix Antibody (1A4) [NBP2-46348] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Alix.
Western Blot: Alix Antibody (1A4) [NBP2-46348] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Alix.
Immunohistochemistry: Alix Antibody (1A4) [NBP2-46348] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Alix Antibody (1A4) [NBP2-46348] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Alix Antibody (1A4) [NBP2-46348] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Alix Antibody (1A4) [NBP2-46348] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Alix Antibody (1A4) [NBP2-46348] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI1A4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-71537AF350
Alexa Fluor 405
NBP2-71537AF405
Alexa Fluor 488
NBP2-71537AF488
Alexa Fluor 532
NBP2-71537AF532
Alexa Fluor 594
NBP2-71537AF594
Alexa Fluor 647
NBP2-71537AF647
Alexa Fluor 700
NBP2-71537AF700
Alexa Fluor 750
NBP2-71537AF750
Allophycocyanin
NBP2-71537APC
Biotin
NBP2-71537B
CoraFluor 1
NBP2-71537CL1
DyLight 350
NBP2-71537UV
DyLight 405
NBP2-71537V
DyLight 488
NBP2-71537G
DyLight 550
NBP2-71537R
DyLight 594
NBP2-71537DL594
DyLight 650
NBP2-71537C
DyLight 680
NBP2-71537FR
DyLight 755
NBP2-71537IR
FITC
NBP2-71537F
HRP
NBP2-71537H
Janelia Fluor 525
NBP2-71537JF525
Janelia Fluor 549
NBP2-71537JF549
Janelia Fluor 646
NBP2-71537JF646
Janelia Fluor 669
NBP2-71537JF669
PE
NBP2-71537PE
PerCP
NBP2-71537PCP
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-71537
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Alix Antibody (OTI1A4) Summary

Immunogen
Full length human recombinant protein of human PDCD6IP(NP_037506) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
PDCD6IP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:4000
Theoretical MW
95.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Alix Antibody (OTI1A4)

  • AIP1DRIP4
  • ALG-2 interacting protein 1
  • ALG-2 interacting protein X
  • ALG-2-interacting protein 1
  • alinx
  • Alix
  • apoptosis-linked gene 2-interacting protein X
  • dopamine receptor interacting protein 4
  • HP95
  • KIAA1375
  • MGC17003
  • PDCD6-interacting protein
  • programmed cell death 6 interacting protein
  • programmed cell death 6-interacting protein

Background

Alix encodes a protein thought to participate in programmed cell death. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization which may be partly responsible for the protection against cell death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Alix Antibody
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NBP2-46348
Alix Antibody (OTI1A4)
Species: Hu, Mu, Rt
Applications: WB, IHC

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Bioinformatics

Gene Symbol PDCD6IP