Alix Antibodies
We offer Alix Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Western Blot. Each Alix Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Alix Antibodies can be used in a variety of model species: Human, Mouse, Rat, Xenopus. Use the list below to choose the Alix Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Alix polyclonal antibodies and browse our Alix monoclonal antibody catalog.
Alternate Names for Alix Antibodies
anti-Alix antibody, anti-PDCD6IP antibody, anti-AIP1DRIP4 antibody, anti-ALG-2 interacting protein 1 antibody, anti-ALG-2 interacting protein X antibody, anti-ALG-2-interacting protein 1 antibody, anti-alinx antibody, anti-apoptosis-linked gene 2-interacting protein X antibody, anti-dopamine receptor interacting protein 4 antibody, anti-HP95 antibody, anti-KIAA1375 antibody, anti-MGC17003 antibody, anti-PDCD6-interacting protein antibody, anti-programmed cell death 6 interacting protein antibody, anti-programmed cell death 6-interacting protein antibody
11 Results for "alix" in Primary Antibodies
![Western Blot: Alix Antibody [NBP1-90201] - Analysis in U-138MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PDCD6IP antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Alix-Antibody-Western-Blot-NBP1-90201-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Alix Antibody [NBP1-90201] - Analysis in U-138MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PDCD6IP antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Alix Antibody [NBP1-90201] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Alix-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90201-img0008.jpg)
![Immunohistochemistry-Paraffin: Alix Antibody [NBP1-90201] - Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Alix-Antibody-Immunohistochemistry-Paraffin-NBP1-90201-img0014.jpg)
![Immunohistochemistry-Paraffin: Alix Antibody [NBP1-90201] - Staining of human gastrointestinal shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Alix-Antibody-Immunohistochemistry-Paraffin-NBP1-90201-img0011.jpg)
![Immunohistochemistry-Paraffin: Alix Antibody [NBP1-90201] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Alix-Antibody-Immunohistochemistry-Paraffin-NBP1-90201-img0012.jpg)
![Immunohistochemistry-Paraffin: Alix Antibody [NBP1-90201] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Alix-Antibody-Immunohistochemistry-Paraffin-NBP1-90201-img0013.jpg)
![Western Blot: Alix Antibody [NBP1-49701] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ALIX antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. ALIX was also immunoprecipitated by rabbit anti-ALIX antibody NBP1-49700, which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/Alix-Antibody-Western-Blot-NBP1-49701-img0001.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Alix Antibody [NBP1-49701] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ALIX antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. ALIX was also immunoprecipitated by rabbit anti-ALIX antibody NBP1-49700, which recognizes an upstream epitope.")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Alix Antibody [NBP1-49701] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Alix Antibody (NBP1-49701) at 1 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Alix-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49701-img0005.jpg)
![Immunohistochemistry-Paraffin: Alix Antibody [NBP1-49701] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- ALIX used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Alix-Antibody-Immunohistochemistry-Paraffin-NBP1-49701-img0002.jpg)
![Flow Cytometry: Alix Antibody [NBP1-49701] - An intracellular stain was performed on NIH3T3 cells with Alix Antibody NBP1-49701 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/Alix-Antibody-Flow-Cytometry-NBP1-49701-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Alix Antibody [NBP1-49701] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Alix Antibody (NBP1-49701) at 1 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Alix-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49701-img0004.jpg)
![Flow Cytometry: Alix Antibody [NBP1-49701] - An intracellular stain was performed on HeLa cells with Alix Antibody NBP1-49701 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/Alix-Antibody-Flow-Cytometry-NBP1-49701-img0006.jpg)
![Simple Western: Alix Antibody - BSA Free [NBP1-49701] - Alix Antibody [NBP1-49701] - Simple Western lane view shows lysates of Exosome Standards (A549 cell line) (NBP3-11645), exosome standards (HT29 cell line) (NBP3-11685), and COLO 205 human colorectal adenocarcinoma cell line whole cell lysate (WCL), loaded at 0.2 mg/mL. A specific band was detected for Alix antibody (NBP1-49701) at approximately 106 kDa (as indicated) using 1:10 of Alix antibody. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Alix-Antibody-Simple-Western-NBP1-49701-img0003.jpg)
![Western Blot: Alix Antibody (1A4) [NBP2-46348] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Alix.](http://images.novusbio.com/fullsize/Alix-Antibody-1A4-Western-Blot-NBP2-46348-img0006.jpg "Western Blot: Alix Antibody (1A4) [NBP2-46348] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Alix.")
![Immunohistochemistry: Alix Antibody (1A4) [NBP2-46348] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Alix-Antibody-1A4-Immunohistochemistry-NBP2-46348-img0005.jpg)
![Immunohistochemistry: Alix Antibody (1A4) [NBP2-46348] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Alix-Antibody-1A4-Immunohistochemistry-NBP2-46348-img0001.jpg)
![Immunohistochemistry: Alix Antibody (1A4) [NBP2-46348] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Alix-Antibody-1A4-Immunohistochemistry-NBP2-46348-img0002.jpg)
![Immunohistochemistry: Alix Antibody (1A4) [NBP2-46348] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Alix-Antibody-1A4-Immunohistochemistry-NBP2-46348-img0003.jpg)
![Immunohistochemistry: Alix Antibody (1A4) [NBP2-46348] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Alix-Antibody-1A4-Immunohistochemistry-NBP2-46348-img0004.jpg)
![Western Blot: Alix Antibody (OTI1A4) - Azide and BSA Free [NBP2-71537] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Alix.](http://images.novusbio.com/fullsize/Alix-Antibody-OTI1A4-Azide-and-BSA-Free-Western-Blot-NBP2-71537-img0001.jpg "Western Blot: Alix Antibody (OTI1A4) - Azide and BSA Free [NBP2-71537] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Alix.")
![Immunohistochemistry: Alix Antibody (OTI1A4) - Azide and BSA Free [NBP2-71537] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Alix-Antibody-OTI1A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71537-img0005.jpg)
![Immunohistochemistry: Alix Antibody (OTI1A4) - Azide and BSA Free [NBP2-71537] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Alix-Antibody-OTI1A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71537-img0002.jpg)
![Immunohistochemistry: Alix Antibody (OTI1A4) - Azide and BSA Free [NBP2-71537] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Alix-Antibody-OTI1A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71537-img0003.jpg)
![Immunohistochemistry: Alix Antibody (OTI1A4) - Azide and BSA Free [NBP2-71537] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Alix-Antibody-OTI1A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71537-img0004.jpg)
![Immunohistochemistry: Alix Antibody (OTI1A4) - Azide and BSA Free [NBP2-71537] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Alix-Antibody-OTI1A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71537-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Alix Antibody (3C4) [H00010015-M01] - Analysis of monoclonal antibody to PDCD6IP on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Alix-Antibody-3C4-Immunocytochemistry-Immunofluorescence-H00010015-M01-img0004.jpg "Immunocytochemistry/Immunofluorescence: Alix Antibody (3C4) [H00010015-M01] - Analysis of monoclonal antibody to PDCD6IP on HeLa cell. Antibody concentration 10 ug/ml.")
![ELISA: Alix Antibody (3C4) [H00010015-M01] - Detection limit for recombinant GST tagged PDCD6IP is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Alix-Antibody-3C4-ELISA-H00010015-M01-img0006.jpg)
![Western Blot: Alix Antibody (7E9) [NBP3-26435] - Positive Western Blot detected in: L02 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, U-87 whole cell lysate, Rat Liver whole cell lysate. All lanes: Alix Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 97, 97, 31 kDa Observed band size: 97 kDa](http://images.novusbio.com/fullsize/nbp3-26435_rabbit-alix-mab-7e9-282202411485823.jpg "Western Blot: Alix Antibody (7E9) [NBP3-26435] - Positive Western Blot detected in: L02 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, U-87 whole cell lysate, Rat Liver whole cell lysate. All lanes: Alix Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 97, 97, 31 kDa Observed band size: 97 kDa")
![Flow Cytometry: Alix Antibody (7E9) [NBP3-26435] - Overlay histogram showing Jurkat cells stained with Alix Antibody (7E9) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26435_rabbit-alix-mab-7e9-282202414312767.jpg)
![Western Blot: Alix Antibody [NBP3-17682] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: RT4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: Tonsil](http://images.novusbio.com/fullsize/Alix-Antibody-Western-Blot-NBP3-17682-img0001.jpg "Western Blot: Alix Antibody [NBP3-17682] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: RT4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: Tonsil")
![Western Blot: Alix Antibody (S09-4B4) [NBP3-19564] - Western blot detection of Alix in HT1080 cell lysates using NBP3-19564 (1:1000 diluted). Predicted band size: 96KDa. Observed band size: 96KDa.](http://images.novusbio.com/fullsize/Alix-Antibody-S09-4B4-Western-Blot-NBP3-19564-img0001.jpg "Western Blot: Alix Antibody (S09-4B4) [NBP3-19564] - Western blot detection of Alix in HT1080 cell lysates using NBP3-19564 (1:1000 diluted). Predicted band size: 96KDa. Observed band size: 96KDa.")