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Alix Antibody (3A9) [DyLight 405]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IP
Clone
3A9
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 405

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-65678AF350
Alexa Fluor 405
NB100-65678AF405
Alexa Fluor 488
NB100-65678AF488
Alexa Fluor 532
NB100-65678AF532
Alexa Fluor 594
NB100-65678AF594
Alexa Fluor 647
NB100-65678AF647
Alexa Fluor 700
NB100-65678AF700
Alexa Fluor 750
NB100-65678AF750
Biotin
NB100-65678B
CoraFluor 1
NB100-65678CL1
DyLight 350
NB100-65678UV
DyLight 488
NB100-65678G
DyLight 550
NB100-65678R
DyLight 594
NB100-65678DL594
DyLight 650
NB100-65678C
DyLight 680
NB100-65678FR
DyLight 755
NB100-65678IR
FITC
NB100-65678F
HRP
NB100-65678H
Janelia Fluor 525
NB100-65678JF525
Janelia Fluor 549
NB100-65678JF549
Janelia Fluor 646
NB100-65678JF646
Janelia Fluor 669
NB100-65678JF669
Unconjugated
NB100-65678
mFluor Violet 450 SE
NB100-65678MFV450
mFluor Violet 500 SE
NB100-65678MFV500
mFluor Violet 610 SE
NB100-65678MFV610
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Alix Antibody (3A9) [DyLight 405] Summary

Description
This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
Immunogen
Alix-GST fusion protein
Specificity
NB100-65678 recognizes apoptotic-linked-gene-product 2 (ALG-2) interacting protein X (ALIX), a conserved adaptor protein which is ubiquitously expressed. Alix was originally reported to play a role in apoptosis but has recently been shown to be involved in other cellular mechanisms including endosomal sorting, endocytosis, viral budding and actin cytoskeleton assembly.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PDCD6IP
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot

Reactivity Notes

Predicted cross-reactivities: Rat, Xenopus, Mouse, Human

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Alix Antibody (3A9) [DyLight 405]

  • AIP1DRIP4
  • ALG-2 interacting protein 1
  • ALG-2 interacting protein X
  • ALG-2-interacting protein 1
  • alinx
  • Alix
  • apoptosis-linked gene 2-interacting protein X
  • dopamine receptor interacting protein 4
  • HP95
  • KIAA1375
  • MGC17003
  • PDCD6-interacting protein
  • programmed cell death 6 interacting protein
  • programmed cell death 6-interacting protein

Background

Alix encodes a protein thought to participate in programmed cell death. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization which may be partly responsible for the protection against cell death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Alix Antibody
(3A9) [DyLight 405]
NB100-65678V
Alix Antibody (3A9) [DyLight 405]
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IP

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PDCD6IP
Entrez
Uniprot