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VPS4A Antibody (SR2511)

Images

 
Western Blot: VPS4A Antibody (SR2511) [NBP3-27784] - Western blot analysis of VPS4A in K562, rat Brain, C6, 3T3 lysates
Immunohistochemistry-Paraffin: VPS4A Antibody (SR2511) [NBP3-27784] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen ...read more
Immunocytochemistry/ Immunofluorescence: VPS4A Antibody (SR2511) [NBP3-27784] - Immunocytochemistry analysis of VPS4A (green) in Hela using NBP3-27784, and DAPI(blue)

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
SR2511
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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VPS4A Antibody (SR2511) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthetic peptide of human VPS4A (Uniprot #: Q9UN37)
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
VPS4A
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:100
  • Western Blot 1:500-1:1000
Theoretical MW
49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
Preservative
0.01% Sodium Azide
Purity
Affinity purified

Alternate Names for VPS4A Antibody (SR2511)

  • FLJ22197
  • hVPS4
  • Protein SKD2
  • SKD1
  • SKD1A
  • SKD2
  • vacuolar protein sorting 4 homolog A (S. cerevisiae)
  • vacuolar protein sorting 4A (yeast homolog)
  • vacuolar protein sorting factor 4A
  • vacuolar protein sorting-associated protein 4A
  • vacuolar sorting protein 4
  • VPS4-1SKD1-homolog
  • VPS4vacuolar protein sorting 4A (yeast)

Background

VPS4A is encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. The mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be really an yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 16; the gene for the other resides on chromosome 18. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol VPS4A