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CHMP5 Antibody (OTI5C7)

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Western Blot: CHMP5 Antibody (5C7) [NBP2-46283] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHMP5.
Western Blot: CHMP5 Antibody (5C7) [NBP2-46283] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHMP5.
Immunohistochemistry: CHMP5 Antibody (5C7) [NBP2-46283] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)
Immunohistochemistry: CHMP5 Antibody (5C7) [NBP2-46283] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)
Immunohistochemistry: CHMP5 Antibody (5C7) [NBP2-46283] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)
Immunohistochemistry: CHMP5 Antibody (5C7) [NBP2-46283] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)
Immunohistochemistry: CHMP5 Antibody (5C7) [NBP2-46283] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)
Immunohistochemistry: CHMP5 Antibody (5C7) [NBP2-46283] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)
Immunohistochemistry: CHMP5 Antibody (5C7) [NBP2-46283] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI5C7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-71937AF350
Alexa Fluor 405
NBP2-71937AF405
Alexa Fluor 488
NBP2-71937AF488
Alexa Fluor 532
NBP2-71937AF532
Alexa Fluor 594
NBP2-71937AF594
Alexa Fluor 647
NBP2-71937AF647
Alexa Fluor 700
NBP2-71937AF700
Alexa Fluor 750
NBP2-71937AF750
Allophycocyanin
NBP2-71937APC
Biotin
NBP2-71937B
CoraFluor 1
NBP2-71937CL1
DyLight 350
NBP2-71937UV
DyLight 405
NBP2-71937V
DyLight 488
NBP2-71937G
DyLight 550
NBP2-71937R
DyLight 594
NBP2-71937DL594
DyLight 650
NBP2-71937C
DyLight 680
NBP2-71937FR
DyLight 755
NBP2-71937IR
FITC
NBP2-71937F
HRP
NBP2-71937H
Janelia Fluor 525
NBP2-71937JF525
Janelia Fluor 549
NBP2-71937JF549
Janelia Fluor 646
NBP2-71937JF646
Janelia Fluor 669
NBP2-71937JF669
PE
NBP2-71937PE
PerCP
NBP2-71937PCP
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-71937
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CHMP5 Antibody (OTI5C7) Summary

Immunogen
Full length human recombinant protein of human CHMP5 (NP_057494) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CHMP5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
Theoretical MW
24.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CHMP5 Antibody (OTI5C7)

  • apoptosis-related protein PNAS-2
  • C9orf83
  • charged multivesicular body protein 5
  • chromatin modifying protein 5
  • Chromatin-modifying protein 5
  • chromosome 9 open reading frame 83
  • HSPC177
  • hVps60
  • PNAS-2
  • SNF7 domain containing 2
  • SNF7 domain-containing protein 2
  • SNF7DC2
  • Vacuolar protein sorting-associated protein 60
  • Vps60CGI-34

Background

CHMP5 is a component of the ESCRT-III complex, which is required for multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-III complex is probably involved in the concentration of MVB cargo. In case of infection, the HIV-1 virus takes advantage of the ESCRT-III complex for budding and exocytic cargoes of viral proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CHMP5