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CHMP5 Antibody (OTI5C7) - Azide and BSA Free

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Western Blot: CHMP5 Antibody (OTI5C7) - Azide and BSA Free [NBP2-71937] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHMP5.
Immunohistochemistry: CHMP5 Antibody (OTI5C7) - Azide and BSA Free [NBP2-71937] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)
Immunohistochemistry: CHMP5 Antibody (OTI5C7) - Azide and BSA Free [NBP2-71937] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)
Immunohistochemistry: CHMP5 Antibody (OTI5C7) - Azide and BSA Free [NBP2-71937] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)
Immunohistochemistry: CHMP5 Antibody (OTI5C7) - Azide and BSA Free [NBP2-71937] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)
Immunohistochemistry: CHMP5 Antibody (OTI5C7) - Azide and BSA Free [NBP2-71937] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)
Immunohistochemistry: CHMP5 Antibody (OTI5C7) - Azide and BSA Free [NBP2-71937] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)
Immunohistochemistry: CHMP5 Antibody (OTI5C7) - Azide and BSA Free [NBP2-71937] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI5C7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

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CHMP5 Antibody (OTI5C7) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human CHMP5 (NP_057494) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CHMP5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
Theoretical MW
24.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for CHMP5 Antibody (OTI5C7) - Azide and BSA Free

  • apoptosis-related protein PNAS-2
  • C9orf83
  • charged multivesicular body protein 5
  • chromatin modifying protein 5
  • Chromatin-modifying protein 5
  • chromosome 9 open reading frame 83
  • HSPC177
  • hVps60
  • PNAS-2
  • SNF7 domain containing 2
  • SNF7 domain-containing protein 2
  • SNF7DC2
  • Vacuolar protein sorting-associated protein 60
  • Vps60CGI-34

Background

CHMP5 is a component of the ESCRT-III complex, which is required for multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-III complex is probably involved in the concentration of MVB cargo. In case of infection, the HIV-1 virus takes advantage of the ESCRT-III complex for budding and exocytic cargoes of viral proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CHMP5