Orthogonal Strategies: Immunohistochemistry-Paraffin: ALDH1A2 Antibody [NBP1-87158] - Staining in human endometrium and skin tissues . Corresponding ALDH1A2 RNA-seq data are presented for the same tissues.
Western Blot: ALDH1A2 Antibody [NBP1-87158] - Analysis in human cell line K562.
Immunohistochemistry-Paraffin: ALDH1A2 Antibody [NBP1-87158] - Staining of human endometrium shows moderate to strong cytoplasmic positivity in cells in endometrial stroma.
Immunohistochemistry-Paraffin: ALDH1A2 Antibody [NBP1-87158] - Staining of human fallopian tube shows moderate to strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: ALDH1A2 Antibody [NBP1-87158] - Staining of human skin shows weak cytoplasmic positivity in a subset of keratinocytes.
Immunohistochemistry-Paraffin: ALDH1A2 Antibody [NBP1-87158] - Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.
Simple Western: ALDH1A2 Antibody [NBP1-87158] - Detection of ALDH1A2 in K562 cells using ALDH1A2 antibody at a dilution of 1:50. K (lane 1) = Wild Type K562, I (lane 2) = Imatinib Resistant K562, N (lane 3) = Nilotinib ...read more
Immunohistochemistry of candidate biomarkers in prostate cancer. Representative immunohistochemical staining of ACPP, ADAM9, ALDH1A2, CASR, CCND1, CCPG1, CD34, CD44, CD44v6, CHGA, CHMP1A, EI24, ENO2, GADD45B, HA, HAS2, ...read more
This antibody was developed against Recombinant Protein corresponding to amino acids: VVGSPFDPTTEQGPQIDKKQYNKILELIQSGVAEGAKLECGGKGLGRKGFFIEPTVFSNVTDDMRIAKEEIFGPVQEILRFKTMDEVIERANNSDFGLVAAVFTNDINKALTVSSAMQAGTV
Marker
Dorso-temporal Retina Marker
Predicted Species
Mouse (98%), Rat (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ALDH1A2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Simple Western Validated from a verified customer review
Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Theoretical MW
57 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified
Alternate Names for ALDH1A2 Antibody
aldehyde dehydrogenase 1 family, member A2
Aldehyde dehydrogenase family 1 member A2
ALDH 1A2
ALDH1A2
EC 1.2.1
EC 1.2.1.36
RALDH 2
RALDH
RALDH(II)
RALDH2
RALDH2MGC26444
RALDH2-T
retinal dehydrogenase 2
Retinaldehyde-specific dehydrogenase type 2
Background
ALDH1A2 belongs to the aldehyde dehydrogenase family of proteins. The product of this gene is an enzyme that catalyzes the synthesis of retinoic acid (RA) from retinaldehyde. Retinoic acid, the active derivative of vitamin A (retinol), is a hormonal signaling molecule that functions in developing and adult tissues. The studies of a similar mouse gene suggest that this enzyme and the cytochrome CYP26A1, concurrently establish local embryonic retinoic acid levels which facilitate posterior organ development and prevent spina bifida. Three transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for ALDH1A2 Antibody (NBP1-87158). (Showing 1 - 1 of 1 FAQs).
I am looking for an anti-human ALDH1A2 antibody to use for flow cytometry and I’m wondering if anyone has used NBP1-87158 for this purpose?
Unfortunately we have not yet investigated the use of product NBP1-87158 in flow cytometry yet. This antibody is validated for use in IHC and ICC, so it is very likely that it would work in flow as well. Since we are not able to cover this application with our guarantee, I can offer you our Innovator's Reward Program.
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