Home » Aldehyde Dehydrogenase 1-A1/ALDH1A1 » Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibodies » Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [Unconjugated]
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Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [Unconjugated]

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Western blot shows lysates of human liver tissue, human testis tissue, and mouse liver. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5869) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Aldehyde Dehydrogenase 1-A1/ALDH1A1 at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Western blot shows lysates of human liver tissue, human testis tissue, and mouse liver. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antigen Affinity-purified ...read more
Aldehyde Dehydrogenase 1-A1/ALDH1A1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antigen Affinity-purified Polyclonal ...read more
Aldehyde Dehydrogenase 1-A1/ALDH1A1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Goat Anti-Human/Mouse Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antigen Affinity-purified ...read more
Simple Western lane view shows lysates of human liver tissue and mouse liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Aldehyde Dehydrogenase 1‑A1/ALDH1A1 at approximately 57 kDa (as indicated) ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, IHC, IP, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF5869-SP
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Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Aldehyde Dehydrogenase 1-A1/ALDH1A1
Ser2-Ser501
Accession # P00352
Specificity
Detects human Aldehyde Dehydrogenase 1‑A1/ALDH1A1 in direct ELISAs and detects human and mouse Aldehyde Dehydrogenase 1‑A1/ALDH1A1 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ALDH1A1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF5869 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [Unconjugated]

  • acetaldehyde dehydrogenase 1
  • ALDC
  • aldehyde dehydrogenase 1 family, member A1
  • aldehyde dehydrogenase 1, soluble
  • Aldehyde Dehydrogenase 1A1
  • Aldehyde Dehydrogenase 1-A1
  • Aldehyde dehydrogenase family 1 member A1
  • Aldehyde dehydrogenase, cytosolic
  • aldehyde dehydrogenase, liver cytosolic
  • ALDH class 1
  • ALDH1
  • ALDH11
  • ALDH1A1
  • ALDH-E1
  • ALHDII
  • EC 1.2.1
  • EC 1.2.1.36
  • MGC2318
  • PUMB1
  • RALDH 1
  • RALDH1
  • retinal dehydrogenase 1
  • retinaldehyde dehydrogenase 1

Background

Aldehyde dehydrogenases (ALDHs) are NAD(P)+-dependent enzymes that detoxify aldehydes by oxidizing them to carboxylic acids. Nineteen ALDHs are present in humans, expressed in a variety of organelles and having different substrate preferences (1). ALDH1A1 is a cytosolic enzyme that preferentially oxidizes retinaldehyde to retinoic acid (2). ALDH1A1 is expressed in the epithelium of many organs, including brain, liver, testis, eye lens and cornea (3). ALDH1A1 is highly expressed in brain dopaminergic neurons, where it produces the retinoic acid required for their differentiation and development (4). The retinoic acid produced by ALDH1A1 is also important for the differentiation of hematopoietic stem cells (5). ALDH1A1 is a major enzyme in the oxidation of acetaldehyde, a toxic metabolite of ethanol (6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (AF5869)(10)

We have publications tested in 3 confirmed species: Human, Mouse, Xenograft.

We have publications tested in 5 applications: Flow Cytometry, ICC, IHC, Immunohistochemistry, Western Blot.


Filter By Application
Flow Cytometry
(1)
ICC
(1)
IHC
(4)
Immunohistochemistry
(1)
Western Blot
(1)
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Human
(3)
Mouse
(4)
Xenograft
(1)
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Showing Publications 1 - 10 of 10.
Publications using AF5869 Applications Species
Rodrigues-Amorim, D;Bozzelli, PL;Kim, T;Liu, L;Gibson, O;Yang, CY;Murdock, MH;Galiana-Melendez, F;Schatz, B;Davison, A;Islam, MR;Shin Park, D;Raju, RM;Abdurrob, F;Nelson, AJ;Min Ren, J;Yang, V;Stokes, MP;Tsai, LH; Multisensory gamma stimulation mitigates the effects of demyelination induced by cuprizone in male mice Nature communications 2024-08-08 [PMID: 39112447] (Immunohistochemistry, Mouse) Immunohistochemistry Mouse
X Liu, Y Ye, L Zhu, X Xiao, B Zhou, Y Gu, H Si, H Liang, M Liu, J Li, Q Jiang, J Li, S Yu, R Ma, S Su, JY Liao, Q Zhao Niche stiffness sustains cancer stemness via TAZ and NANOG phase separation Nature Communications, 2023-01-16;14(1):238. 2023-01-16 [PMID: 36646707] (IHC, Human) IHC Human
W Zeng, L Xiong, W Wu, S Li, J Liu, L Yang, L Lao, P Huang, M Zhang, H Chen, N Miao, Z Lin, Z Liu, X Yang, J Wang, P Wang, E Song, Y Yao, Y Nie, J Chen, D Huang CCL18 signaling from tumor-associated macrophages activates fibroblasts to adopt a chemoresistance-inducing phenotype Oncogene, 2022-11-22;0(0):. 2022-11-22 [PMID: 36418470] (Flow Cytometry, Xenograft) Flow Cytometry Xenograft
Riccardo Melani, Nicolas X. Tritsch Inhibitory co-transmission from midbrain dopamine neurons relies on presynaptic GABA uptake Cell Reports 4/19/2022 [PMID: 35443174]
J Kim, J Jeon, B Song, N Lee, S Ko, Y Cha, P Leblanc, H Seo, KS Kim Spotting-based differentiation of functional dopaminergic progenitors from human pluripotent stem cells Nature Protocols, 2022-02-09;0(0):. 2022-02-09 [PMID: 35140411] (ICC, Human) ICC Human
M Pereira Lu, M Azcorra, G Caronia-Br, JF Poulin, Z Gaertner, S Gatica, OA Moreno-Ram, N Nouri, M Dubois, YC Ma, C Ramakrishn, L Fenno, YS Kim, K Deisseroth, F Cicchetti, DA Dombeck, R Awatramani Sox6 expression distinguishes dorsally and ventrally biased dopamine neurons in the substantia nigra with distinctive properties and embryonic origins Cell Reports, 2021-11-09;37(6):109975. 2021-11-09 [PMID: 34758317] (IHC, Mouse) IHC Mouse
Yinan Jiang, Yichen Guo, Jinjin Hao, Rachael Guenter, Justin Lathia, Adam W. Beck et al. Development of an arteriolar niche and self-renewal of breast cancer stem cells by lysophosphatidic acid/protein kinase D signaling Communications Biology 6/24/2021 [PMID: 34168243]
J Wu, J Kung, J Dong, L Chang, C Xie, A Habib, S Hawes, N Yang, V Chen, Z Liu, R Evans, B Liang, L Sun, J Ding, J Yu, S Saez-Atien, B Tang, Z Khaliq, DT Lin, W Le, H Cai Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning Cell Rep, 2019-07-30;28(5):1167-1181.e7. 2019-07-30 [PMID: 31365862] (IHC, Mouse) IHC Mouse
C Sgobio, J Wu, W Zheng, X Chen, J Pan, AG Salinas, MI Davis, DM Lovinger, H Cai Aldehyde dehydrogenase 1-positive nigrostriatal dopaminergic fibers exhibit distinct projection pattern and dopamine release dynamics at mouse dorsal striatum Sci Rep, 2017-07-13;7(1):5283. 2017-07-13 [PMID: 28706191] (IHC, Mouse) IHC Mouse
W Cai, G Chen, Q Luo, J Liu, X Guo, T Zhang, F Ma, L Yuan, B Li, J Cai PMP22 Regulates Self-Renewal and Chemoresistance of Gastric Cancer Cells Mol. Cancer Ther, 2017-03-23;0(0):. 2017-03-23 [PMID: 28336807] (Western Blot, Human) Western Blot Human

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Bioinformatics

Gene Symbol ALDH1A1
Uniprot