![Western Blot: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - Analysis of extracts (10ug) from 9 Human tissue by using anti-ADH1B monoclonal antibody at 1:1000 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).](http://images.novusbio.com/fullsize/alcohol-dehydrogenase-1B-Antibody-OTI4F12-Western-Blot-NBP2-00649-img0015.jpg "Western Blot: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - Analysis of extracts (10ug) from 9 Human tissue by using anti-ADH1B monoclonal antibody at 1:1000 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).")
| Reactivity | Hu, Rt, CaSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | OTI4F12 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.89 mg/ml |
| Immunogen | Full length human recombinant protein of human ADH1B (NP_000659) produced in HEK293T cell. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ADH1B |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 39.7 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.89 mg/ml |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY alcohol dehydrogenase 1B.](http://images.novusbio.com/fullsize/alcohol-dehydrogenase-1B-Antibody-4F12-Immunocytochemistry-Immunofluorescence-NBP2-00649-img0013.jpg "Immunocytochemistry/Immunofluorescence: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY alcohol dehydrogenase 1B.")
![Immunohistochemistry-Paraffin: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - Staining of paraffin-embedded Human lymph node tissue using anti-alcohol dehydrogenase 1B mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alcohol-dehydrogenase-1B-Antibody-4F12-Immunohistochemistry-Paraffin-NBP2-00649-img0012.jpg "Immunohistochemistry-Paraffin: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - Staining of paraffin-embedded Human lymph node tissue using anti-alcohol dehydrogenase 1B mouse monoclonal antibody.")
![Flow Cytometry: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-alcohol dehydrogenase 1B antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/alcohol-dehydrogenase-1B-Antibody-4F12-Flow-Cytometry-NBP2-00649-img0003.jpg "Flow Cytometry: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-alcohol dehydrogenase 1B antibody, and then analyzed by flow cytometry.")
![Western Blot: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY alcohol dehydrogenase 1B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-alcohol dehydrogenase 1B.](http://images.novusbio.com/fullsize/alcohol-dehydrogenase-1B-Antibody-4F12-Western-Blot-NBP2-00649-img0004.jpg "Western Blot: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY alcohol dehydrogenase 1B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-alcohol dehydrogenase 1B.")
![Western Blot: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - Analysis of extracts (35ug) from 9 different cell lines by using anti-alcohol dehydrogenase 1B monoclonal antibody.](http://images.novusbio.com/fullsize/alcohol-dehydrogenase-1B-Antibody-4F12-Western-Blot-NBP2-00649-img0014.jpg "Western Blot: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - Analysis of extracts (35ug) from 9 different cell lines by using anti-alcohol dehydrogenase 1B monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - Immunofluorescent staining of HeLa cells using anti-alcohol dehydrogenase 1B mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alcohol-dehydrogenase-1B-Antibody-4F12-Immunocytochemistry-Immunofluorescence-NBP2-00649-img0005.jpg "Immunocytochemistry/Immunofluorescence: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - Immunofluorescent staining of HeLa cells using anti-alcohol dehydrogenase 1B mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-alcohol dehydrogenase 1B mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alcohol-dehydrogenase-1B-Antibody-4F12-Immunohistochemistry-Paraffin-NBP2-00649-img0006.jpg "Immunohistochemistry-Paraffin: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-alcohol dehydrogenase 1B mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-alcohol dehydrogenase 1B mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alcohol-dehydrogenase-1B-Antibody-4F12-Immunohistochemistry-Paraffin-NBP2-00649-img0007.jpg "Immunohistochemistry-Paraffin: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-alcohol dehydrogenase 1B mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-alcohol dehydrogenase 1B mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alcohol-dehydrogenase-1B-Antibody-4F12-Immunohistochemistry-Paraffin-NBP2-00649-img0008.jpg "Immunohistochemistry-Paraffin: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-alcohol dehydrogenase 1B mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-alcohol dehydrogenase 1B mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alcohol-dehydrogenase-1B-Antibody-4F12-Immunohistochemistry-Paraffin-NBP2-00649-img0009.jpg "Immunohistochemistry-Paraffin: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-alcohol dehydrogenase 1B mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - Staining of paraffin-embedded Human liver tissue using anti-alcohol dehydrogenase 1B mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alcohol-dehydrogenase-1B-Antibody-4F12-Immunohistochemistry-Paraffin-NBP2-00649-img0010.jpg "Immunohistochemistry-Paraffin: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - Staining of paraffin-embedded Human liver tissue using anti-alcohol dehydrogenase 1B mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - Staining of paraffin-embedded Human lung tissue using anti-alcohol dehydrogenase 1B mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alcohol-dehydrogenase-1B-Antibody-4F12-Immunohistochemistry-Paraffin-NBP2-00649-img0011.jpg "Immunohistochemistry-Paraffin: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - Staining of paraffin-embedded Human lung tissue using anti-alcohol dehydrogenase 1B mouse monoclonal antibody.")
![Flow Cytometry: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - Analysis of Hela cells, using anti-alcohol dehydrogenase 1B antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/alcohol-dehydrogenase-1B-Antibody-4F12-Flow-Cytometry-NBP2-00649-img0001.jpg "Flow Cytometry: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - Analysis of Hela cells, using anti-alcohol dehydrogenase 1B antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
![Flow Cytometry: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - Analysis of Jurkat cells, using anti-alcohol dehydrogenase 1B antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/alcohol-dehydrogenase-1B-Antibody-4F12-Flow-Cytometry-NBP2-00649-img0002.jpg "Flow Cytometry: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - Analysis of Jurkat cells, using anti-alcohol dehydrogenase 1B antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
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