![Western Blot: ALDH1A3 Antibody - BSA Free [NBP2-15339] - Total protein from human Lymph node, mouse Stomach and A431 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-ALDH1A3 (NBP2-15339) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/ALDH1A3-Antibody---BSA-Free-Western-Blot-NBP2-15339-img0021.jpg "Western Blot: ALDH1A3 Antibody - BSA Free [NBP2-15339] - Total protein from human Lymph node, mouse Stomach and A431 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-ALDH1A3 (NBP2-15339) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
| Reactivity | Hu, Mu, Rt, RMSpecies Glossary |
| Applications | WB, ICC/IF, IHC, WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ALDH1A3. The exact sequence is proprietary. |
| Localization | Cytoplasm |
| Predicted Species | Rhesus Macaque (98%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ALDH1A3 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 56 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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reviewed by:
Verified Customer |
WB | Human | 03/08/2020 |
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reviewed by:
Verified Customer |
ICC | Human | 01/14/2020 |
Summary
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reviewed by:
Verified Customer |
IHC-Fr | Mouse | 11/09/2016 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for ALDH1A3 Antibody (NBP2-15339)Find related products by research area.
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| Verified Customer 03/08/2020 |
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| Application: | WB | |
| Species: | Human |
| Verified Customer 01/14/2020 |
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| Application: | ICC | |
| Species: | Human |
| Verified Customer 11/09/2016 |
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| Application: | IHC-Fr | |
| Species: | Mouse |
![Immunocytochemistry/Immunofluorescence: ALDH1A3 Antibody - BSA Free [NBP2-15339] - A431 cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-ALDH1A3 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/ALDH1A3-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-15339-img0022.jpg "Immunocytochemistry/Immunofluorescence: ALDH1A3 Antibody - BSA Free [NBP2-15339] - A431 cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-ALDH1A3 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Immunohistochemistry: ALDH1A3 Antibody - BSA Free [NBP2-15339] - Markers of dedifferentiation are present in islets of db/db, but not weight-matched WD-fed mice. Staining for Aldh1a3 protein (red) and insulin (green) in islets from weight-matched WD-fed mice (20 weeks on diet) versus db/db mice (14 weeks of age). Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/jdr/2017/8503754/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ALDH1A3-Antibody---BSA-Free-Immunohistochemistry-NBP2-15339-img0020.jpg "Immunohistochemistry: ALDH1A3 Antibody - BSA Free [NBP2-15339] - Markers of dedifferentiation are present in islets of db/db, but not weight-matched WD-fed mice. Staining for Aldh1a3 protein (red) and insulin (green) in islets from weight-matched WD-fed mice (20 weeks on diet) versus db/db mice (14 weeks of age). Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/jdr/2017/8503754/) licensed under a CC-BY license.")
![Western Blot: ALDH1A3 Antibody - BSA Free [NBP2-15339] - IL25 Deficiency Induced loss of CRC stemness. The expression levels of CSC markers, namely, DCLK1, ALDH1 (NBP2-15339), and CD44, were examined in tumor tissues by Western blotting. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35359953/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ALDH1A3 Antibody - BSA Free-Western Blot-NBP2-15339-img0023.jpg "Western Blot: ALDH1A3 Antibody - BSA Free [NBP2-15339] - IL25 Deficiency Induced loss of CRC stemness. The expression levels of CSC markers, namely, DCLK1, ALDH1 (NBP2-15339), and CD44, were examined in tumor tissues by Western blotting. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35359953/) licensed under a CC-BY license.")
![Western Blot: ALDH1A3 Antibody - BSA Free [NBP2-15339] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ALDH1A3-Antibody---BSA-Free-Western-Blot-NBP2-15339-img0010.jpg "Western Blot: ALDH1A3 Antibody - BSA Free [NBP2-15339] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: ALDH1A3 Antibody - BSA Free [NBP2-15339] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], diluted at 1:2000.](http://images.novusbio.com/fullsize/ALDH1A3-Antibody---BSA-Free-Western-Blot-NBP2-15339-img0011.jpg "Western Blot: ALDH1A3 Antibody - BSA Free [NBP2-15339] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], diluted at 1:2000.")
![Immunocytochemistry/Immunofluorescence: ALDH1A3 Antibody - BSA Free [NBP2-15339] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ALDH1A3 stained by ALDH1A3 antibody [N2C2], diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/ALDH1A3-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-15339-img0014.jpg "Immunocytochemistry/Immunofluorescence: ALDH1A3 Antibody - BSA Free [NBP2-15339] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ALDH1A3 stained by ALDH1A3 antibody [N2C2], diluted at 1:500. Blue: Hoechst 33342 staining.")
![Immunocytochemistry/Immunofluorescence: ALDH1A3 Antibody - BSA Free [NBP2-15339] - Human breast cancer cells with Aldh1a3 overexpression were stained at 1:500 in 5% goat serum in PBS-T. Human breast cancer cells were transduced with human Aldh1a3 and probed with antibody at 1:500 overnight at 4C followed by counterstaining with secondary and DAPI. Strong signal observed compared to knockout controls. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/ALDH1A3-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-15339-img0019.jpg "Immunocytochemistry/Immunofluorescence: ALDH1A3 Antibody - BSA Free [NBP2-15339] - Human breast cancer cells with Aldh1a3 overexpression were stained at 1:500 in 5% goat serum in PBS-T. Human breast cancer cells were transduced with human Aldh1a3 and probed with antibody at 1:500 overnight at 4C followed by counterstaining with secondary and DAPI. Strong signal observed compared to knockout controls. ICC/IF image submitted by a verified customer review.")
![Immunohistochemistry-Frozen: ALDH1A3 Antibody - BSA Free [NBP2-15339] - 8-week old mouse pancreas cryosections with beta cell dedifferentiation properties were stained with ALDH1A3 (1:500 dilution) and Insulin (1:1000 dilution) antibodies. Blue arrows indicate insulin+Aldh1A3+ cells. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/ALDH1A3-Antibody---BSA-Free-Immunohistochemistry-Frozen-NBP2-15339-img0007.jpg "Immunohistochemistry-Frozen: ALDH1A3 Antibody - BSA Free [NBP2-15339] - 8-week old mouse pancreas cryosections with beta cell dedifferentiation properties were stained with ALDH1A3 (1:500 dilution) and Insulin (1:1000 dilution) antibodies. Blue arrows indicate insulin+Aldh1A3+ cells. IHC-Fr image submitted by a verified customer review.")
![Immunohistochemistry-Paraffin: ALDH1A3 Antibody - BSA Free [NBP2-15339] - Mouse prostate. ALDH1A3 stained by ALDH1A3 antibody [N2C2], diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/ALDH1A3-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP2-15339-img0015.jpg "Immunohistochemistry-Paraffin: ALDH1A3 Antibody - BSA Free [NBP2-15339] - Mouse prostate. ALDH1A3 stained by ALDH1A3 antibody [N2C2], diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: ALDH1A3 Antibody - BSA Free [NBP2-15339] - Rat prostate. ALDH1A3 stained by ALDH1A3 antibody [N2C2], diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/ALDH1A3-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP2-15339-img0016.jpg "Immunohistochemistry-Paraffin: ALDH1A3 Antibody - BSA Free [NBP2-15339] - Rat prostate. ALDH1A3 stained by ALDH1A3 antibody [N2C2], diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
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![Immunohistochemistry Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Aldehyde_Dehydrogenase_1-A1_AF5869_Immunohistochemistry_10638.jpg)
![Simple Western Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Aldehyde_Dehydrogenase_1A1_AF5869_Simple_Western_17067.jpg)
![SDS-PAGE BMP-4 [Unconjugated]](https://images.novusbio.com/images2/BMP-4_314-BP_100.jpg)
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![Simple Western Pax6 Antibody [Unconjugated]](https://images.novusbio.com/images2/Pax6_AF8150_Simple_Western_17126.jpg)
![Immunocytochemistry Pax6 Antibody [Unconjugated]](https://images.novusbio.com/images2/Pax6_AF8150_Immunocytochemistry_14427.jpg)
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![SDS-PAGE FGF-8 [Unconjugated]](https://images.novusbio.com/images2/FGF-8_423-F8_332.jpg)
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![SDS-PAGE Sonic Hedgehog/Shh [Unconjugated]](https://images.novusbio.com/images2/Sonic_Hedgehog_464-SH_341.jpg)
![SEC-MALS Sonic Hedgehog/Shh [Unconjugated]](https://images.novusbio.com/images/464-sh_recombinant-mouse-sonic-hedgehog-shh-c25ii-n-terminus-sec-mals-642023224324.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
