![Western Blot: AKR1A1 Antibody (OTI6E3) - Azide and BSA Free [NBP2-71591] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKR1A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKR1A1.](http://images.novusbio.com/fullsize/AKR1A1-Antibody-OTI6E3-Azide-and-BSA-Free-Western-Blot-NBP2-71591-img0001.jpg "Western Blot: AKR1A1 Antibody (OTI6E3) - Azide and BSA Free [NBP2-71591] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKR1A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKR1A1.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | OTI6E3 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Full length human recombinant protein of human AKR1A1 (NP_006057) produced in HEK293T cell. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | AKR1A1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 36.6 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
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Secondary Antibodies |
Isotype Controls |
Research Areas for AKR1A1 Antibody (NBP2-71591)Find related products by research area.
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| Gene Symbol | AKR1A1 |
![Immunocytochemistry/Immunofluorescence: AKR1A1 Antibody (OTI6E3) - Azide and BSA Free [NBP2-71591] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY AKR1A1.](http://images.novusbio.com/fullsize/AKR1A1-Antibody-OTI6E3-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-71591-img0006.jpg "Immunocytochemistry/Immunofluorescence: AKR1A1 Antibody (OTI6E3) - Azide and BSA Free [NBP2-71591] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY AKR1A1.")
![Immunohistochemistry: AKR1A1 Antibody (OTI6E3) - Azide and BSA Free [NBP2-71591] - Staining of paraffin-embedded liver tissue using anti-AKR1A1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AKR1A1-Antibody-OTI6E3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71591-img0007.jpg "Immunohistochemistry: AKR1A1 Antibody (OTI6E3) - Azide and BSA Free [NBP2-71591] - Staining of paraffin-embedded liver tissue using anti-AKR1A1 mouse monoclonal antibody.")
![Flow Cytometry: AKR1A1 Antibody (OTI6E3) - Azide and BSA Free [NBP2-71591] - Analysis of Jurkat cells, using anti-AKR1A1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/AKR1A1-Antibody-OTI6E3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71591-img0003.jpg "Flow Cytometry: AKR1A1 Antibody (OTI6E3) - Azide and BSA Free [NBP2-71591] - Analysis of Jurkat cells, using anti-AKR1A1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).")
![Immunocytochemistry/Immunofluorescence: AKR1A1 Antibody (OTI6E3) - Azide and BSA Free [NBP2-71591] - Staining of HepG2 cells using anti-AKR1A1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AKR1A1-Antibody-OTI6E3-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-71591-img0005.jpg "Immunocytochemistry/Immunofluorescence: AKR1A1 Antibody (OTI6E3) - Azide and BSA Free [NBP2-71591] - Staining of HepG2 cells using anti-AKR1A1 mouse monoclonal antibody.")
![Flow Cytometry: AKR1A1 Antibody (OTI6E3) - Azide and BSA Free [NBP2-71591] - Analysis of Hela cells, using anti-AKR1A1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/AKR1A1-Antibody-OTI6E3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71591-img0002.jpg "Flow Cytometry: AKR1A1 Antibody (OTI6E3) - Azide and BSA Free [NBP2-71591] - Analysis of Hela cells, using anti-AKR1A1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).")
![CyTOF-ready: AKR1A1 Antibody (OTI6E3) - Azide and BSA Free [NBP2-71591] - HEK293T cells transfected with either pCMV6-ENTRY AKR1A1.(Red) or empty vector control plasmid (Blue) were immunostaining with anti-AKR1A1 mouse monoclonal, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/AKR1A1-Antibody-OTI6E3-Azide-and-BSA-Free-CyTOF-ready-NBP2-71591-img0004.jpg "CyTOF-ready: AKR1A1 Antibody (OTI6E3) - Azide and BSA Free [NBP2-71591] - HEK293T cells transfected with either pCMV6-ENTRY AKR1A1.(Red) or empty vector control plasmid (Blue) were immunostaining with anti-AKR1A1 mouse monoclonal, and then analyzed by flow cytometry.")
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