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![Western Blot: AKR1A1 Antibody (6E3) [NBP2-02164] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKR1A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKR1A1.](http://images.novusbio.com/fullsize/AKR1A1-Antibody-6E3-Western-Blot-NBP2-02164-img0004.jpg "Western Blot: AKR1A1 Antibody (6E3) [NBP2-02164] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKR1A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKR1A1.")
![Immunocytochemistry/Immunofluorescence: AKR1A1 Antibody (6E3) [NBP2-02164] Staining of COS7 cells transiently transfected by pCMV6-ENTRY AKR1A1.](http://images.novusbio.com/fullsize/AKR1A1-Antibody-6E3-Immunocytochemistry-Immunofluorescence-NBP2-02164-img0007.jpg)
![Immunohistochemistry-Paraffin: AKR1A1 Antibody (6E3) [NBP2-02164] - Staining of paraffin-embedded liver tissue using anti-AKR1A1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AKR1A1-Antibody-6E3-Immunohistochemistry-Paraffin-NBP2-02164-img0006.jpg)
![Flow Cytometry: AKR1A1 Antibody (6E3) [NBP2-02164] - HEK293T cells transfected with either pCMV6-ENTRY AKR1A1.(Red) or empty vector control plasmid (Blue) were immunostained with anti-AKR1A1 mouse monoclonal, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/AKR1A1-Antibody-6E3-Flow-Cytometry-NBP2-02164-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: AKR1A1 Antibody (6E3) [NBP2-02164] - Immunofluorescent staining of HepG2 cells using anti-AKR1A1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AKR1A1-Antibody-6E3-Immunocytochemistry-Immunofluorescence-NBP2-02164-img0005.jpg)
![Flow Cytometry: AKR1A1 Antibody (6E3) [NBP2-02164] - Analysis of Hela cells, using anti-AKR1A1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/AKR1A1-Antibody-6E3-Flow-Cytometry-NBP2-02164-img0001.jpg)
![Flow Cytometry: AKR1A1 Antibody (6E3) [NBP2-02164] - Analysis of Jurkat cells, using anti-AKR1A1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/AKR1A1-Antibody-6E3-Flow-Cytometry-NBP2-02164-img0002.jpg)
![Western Blot: AKR1A1 Antibody (1A11-2A4) [H00010327-M01] - AKR1A1 monoclonal antibody (M01), clone 1A11-2A4 Analysis of AKR1A1 expression in HL-60.](http://images.novusbio.com/fullsize/AKR1A1-Antibody-1A11-2A4-Western-Blot-H00010327-M01-img0008.jpg "Western Blot: AKR1A1 Antibody (1A11-2A4) [H00010327-M01] - AKR1A1 monoclonal antibody (M01), clone 1A11-2A4 Analysis of AKR1A1 expression in HL-60.")
![Immunohistochemistry-Paraffin: AKR1A1 Antibody (1A11-2A4) [H00010327-M01] - Analysis of monoclonal antibody to AKR1A1 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 0.75 ug/ml.](http://images.novusbio.com/fullsize/AKR1A1-Antibody-1A11-2A4-Immunohistochemistry-Paraffin-H00010327-M01-img0006.jpg)
![Western Blot: AKR1A1 Antibody (1A11-2A4) [H00010327-M01] - Analysis of AKR1A1 expression in transfected 293T cell line by AKR1A1 monoclonal antibody (M01), clone 1A11-2A4.Lane 1: AKR1A1 transfected lysate(36.6 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/AKR1A1-Antibody-1A11-2A4-Western-Blot-H00010327-M01-img0009.jpg)
![Sandwich ELISA: AKR1A1 Antibody (1A11-2A4) [H00010327-M01] - Detection limit for recombinant GST tagged AKR1A1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/AKR1A1-Antibody-1A11-2A4-Sandwich-ELISA-H00010327-M01-img0007.jpg)
![Immunohistochemistry: AKR1A1 Antibody [NBP3-35364] - Immunohistochemistry analysis of paraffin-embedded Rat kidney using AKR1A1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35364_rabbit-akr1a1-pab-1012202411545531.jpg "Immunohistochemistry: AKR1A1 Antibody [NBP3-35364] - Immunohistochemistry analysis of paraffin-embedded Rat kidney using AKR1A1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunocytochemistry/ Immunofluorescence: AKR1A1 Antibody [NBP3-35364] - Immunofluorescence analysis of HeLa cells using AKR1A1 Rabbit pAb.Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution.](http://images.novusbio.com/fullsize/nbp3-35364_rabbit-akr1a1-pab-10122024121026.jpg)
![Immunohistochemistry: AKR1A1 Antibody [NBP3-35364] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using AKR1A1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35364_rabbit-akr1a1-pab-10122024120626.jpg)
![Western Blot: AKR1A1 Antibody [NBP3-35364] - Western blot analysis of various lysates using AKR1A1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/nbp3-35364_rabbit-akr1a1-pab-101220241214410.jpg)
![Western Blot: AKR1A1 Antibody [NB100-1074] - Analysis of AKR1A1 in Human Placenta lysate using NB100-1074 at 1 ug/ ml.](http://images.novusbio.com/fullsize/AKR1A1-Antibody-Western-Blot-NB100-1074-img0005.jpg "Western Blot: AKR1A1 Antibody [NB100-1074] - Analysis of AKR1A1 in Human Placenta lysate using NB100-1074 at 1 ug/ ml.")
![Immunohistochemistry-Paraffin: AKR1A1 Antibody [NB100-1074] - (5ug/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/AKR1A1-Antibody-Immunohistochemistry-Paraffin-NB100-1074-img0007.jpg)
![Immunohistochemistry-Paraffin: AKR1A1 Antibody [NB100-1074] - IHC staining of AKR1A1 in paraffin embedded Human Kidney using this antibody at 2.5g/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/AKR1A1-Antibody-Immunohistochemistry-Paraffin-NB100-1074-img0003.jpg)
![Immunohistochemistry-Paraffin: AKR1A1 Antibody [NB100-1074] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.](http://images.novusbio.com/fullsize/AKR1A1-Antibody-Immunohistochemistry-Paraffin-NB100-1074-img0006.jpg)
![Western Blot: AKR1A1 Antibody [NBP1-89121] - Analysis in control (vector only transfected HEK293T lysate) and AKR1A1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/AKR1A1-Antibody-Western-Blot-NBP1-89121-img0009.jpg "Western Blot: AKR1A1 Antibody [NBP1-89121] - Analysis in control (vector only transfected HEK293T lysate) and AKR1A1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunocytochemistry/Immunofluorescence: AKR1A1 Antibody [NBP1-89121] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/AKR1A1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89121-img0008.jpg)
![Immunohistochemistry-Paraffin: AKR1A1 Antibody [NBP1-89121] - Staining of human pancreas shows cytoplasmic positivity both in exocrine glandular cells and islet cells.](http://images.novusbio.com/fullsize/AKR1A1-Antibody-Immunohistochemistry-Paraffin-NBP1-89121-img0002.jpg)
![Western Blot: AKR1A1 Antibody [NBP1-89120] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/AKR1A1-Antibody-Western-Blot-NBP1-89120-img0005.jpg "Western Blot: AKR1A1 Antibody [NBP1-89120] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).")
![Immunohistochemistry-Paraffin: AKR1A1 Antibody [NBP1-89120] - Staining of human pancreas shows strong nuclear positivity in exocrine cells.](http://images.novusbio.com/fullsize/AKR1A1-Antibody-Immunohistochemistry-Paraffin-NBP1-89120-img0002.jpg)
![Western Blot: AKR1A1 Antibody [NBP1-89120] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-27](http://images.novusbio.com/fullsize/AKR1A1-Antibody-Western-Blot-NBP1-89120-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: AKR1A1 Antibody [NBP1-89122] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.](http://images.novusbio.com/fullsize/AKR1A1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89122-img0004.jpg "Immunocytochemistry/Immunofluorescence: AKR1A1 Antibody [NBP1-89122] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.")
![Immunohistochemistry-Paraffin: AKR1A1 Antibody [NBP1-89122] - Staining of human kidney shows strong cytoplasmic and nuclear positivity in cells in tubules.](http://images.novusbio.com/fullsize/AKR1A1-Antibody-Immunohistochemistry-Paraffin-NBP1-89122-img0005.jpg)
![Western Blot: AKR1A1 Antibody [NBP3-35161] - Western blot analysis of various lysates using AKR1A1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/nbp3-35161_rabbit-akr1a1-pab-101220241155594.jpg "Western Blot: AKR1A1 Antibody [NBP3-35161] - Western blot analysis of various lysates using AKR1A1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.")
![Knockout Validated: AKR1A1 Antibody [NBP3-03447] - Analysis of extracts from normal (control) and AKR1A1 knockout (KO) 293T cells, using AKR1A1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/AKR1A1-Antibody-Knockout-Validated-NBP3-03447-img0001.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: AKR1A1 Antibody [NBP3-03447] - Analysis of extracts from normal (control) and AKR1A1 knockout (KO) 293T cells, using AKR1A1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![Western Blot: AKR1A1 Antibody [NBP2-15321] - Sample (30 ug of whole cell lysate) A: A549 B: HepG2 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/AKR1A1-Antibody-Western-Blot-NBP2-15321-img0001.jpg "Western Blot: AKR1A1 Antibody [NBP2-15321] - Sample (30 ug of whole cell lysate) A: A549 B: HepG2 10% SDS PAGE; antibody diluted at 1:1000.")
![Western Blot: AKR1A1 Overexpression Lysate (Adult Normal) [NBL1-07433] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for AKR1A1.](http://images.novusbio.com/fullsize/AKR1A1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07433-img0002.jpg "Western Blot: AKR1A1 Overexpression Lysate (Adult Normal) [NBL1-07433] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for AKR1A1.")
![Western Blot: AKR1A1 Overexpression Lysate (Adult Normal) [NBL1-07432] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for AKR1A1.](http://images.novusbio.com/fullsize/AKR1A1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07432-img0002.jpg "Western Blot: AKR1A1 Overexpression Lysate (Adult Normal) [NBL1-07432] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for AKR1A1.")