![Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) - Azide and BSA Free [NBP2-80552] - A surface stain was performed on U87 MG cells with 5'-Nucleotidase/CD73 Antibody (AD2) antibody NBP2-48480 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by Dylight488-conjugated anti-mouse secondary antibody. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-AD2-Azide-and-BSA-Free-Flow-Cell-Surface-NBP2-80552-img0003.jpg "Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) - Azide and BSA Free [NBP2-80552] - A surface stain was performed on U87 MG cells with 5'-Nucleotidase/CD73 Antibody (AD2) antibody NBP2-48480 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by Dylight488-conjugated anti-mouse secondary antibody. Image from the standard format of this antibody.")
| Reactivity | HuSpecies Glossary |
| Applications | Flow, IHC, CyTOF-ready |
| Clone | AD2 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Additional Information | Clone AD2 was used by HLDA to establish CD designation. |
| Immunogen | Human CD73 [UniProt# P21589] |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | NT5E |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for 5'-Nucleotidase/CD73 Antibody (NBP2-80552)Find related products by research area.
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Adenosine Inhibits T cell Tumor Infiltration: KCa3.1, a New Anticancer Target By Yoskaly Lazo-Fernandez, PhD Role of Adenosine in the Tumor Microenvironment a Target for Cancer TherapyThe tumor microenvironment (TME) tends to be concentrated in the purine nucleoside adenosine, a direct resu... Read full blog post. |
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CD73 (Cluster of differentiation 73, ecto-5'-nucleotidase) CD73 is a 70kD glycosyl-phosphatidylinositol (GPI)-anchored cell surface molecule that belongs to the 5'-nucleosidase family. It hydrolyzes extracellular nucleotides into membrane permeable nucleosides and is found as both a membrane-bound and soluble... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | NT5E |
![Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) - Azide and BSA Free [NBP2-80552] - A cell surface stain was performed on U87MG cells with NBP2-48480APC (blue) and a matched isotype control NBP2-27287APC (orange). Cells were incubated in an antibody dilution of 0.5 ug/mL for 20 minutes at room temperature. Both antibodies were directly conjugated to Allophycocyanin. Image using the Allophycocyanin form of this antibody.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-AD2-Azide-and-BSA-Free-Flow-Cell-Surface-NBP2-80552-img0001.jpg "Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) - Azide and BSA Free [NBP2-80552] - A cell surface stain was performed on U87MG cells with NBP2-48480APC (blue) and a matched isotype control NBP2-27287APC (orange). Cells were incubated in an antibody dilution of 0.5 ug/mL for 20 minutes at room temperature. Both antibodies were directly conjugated to Allophycocyanin. Image using the Allophycocyanin form of this antibody.")
![Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) - Azide and BSA Free [NBP2-80552] - A surface stain was performed on human peripheral blood lymphocytes with CD73 (AD2) antibody NBP2-48480AF488 and a matched isotype control NBP2-27287AF488. Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-25196AF647. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-AD2-Azide-and-BSA-Free-Flow-Cell-Surface-NBP2-80552-img0002.jpg "Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) - Azide and BSA Free [NBP2-80552] - A surface stain was performed on human peripheral blood lymphocytes with CD73 (AD2) antibody NBP2-48480AF488 and a matched isotype control NBP2-27287AF488. Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-25196AF647. Image from the standard format of this antibody.")
![Flow Cytometry: 5'-Nucleotidase/CD73 Antibody (AD2) - Azide and BSA Free [NBP2-80552] - A surface stain was performed on CD3+ human peripheral blood lymphocytes with CD73 (AD2) antibody NBP2-48480AF488 and a matched isotype control NBP2-27287AF488. Cells were incubated in an antibody dilution of 10 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-25186PCP. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-AD2-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80552-img0004.jpg "Flow Cytometry: 5'-Nucleotidase/CD73 Antibody (AD2) - Azide and BSA Free [NBP2-80552] - A surface stain was performed on CD3+ human peripheral blood lymphocytes with CD73 (AD2) antibody NBP2-48480AF488 and a matched isotype control NBP2-27287AF488. Cells were incubated in an antibody dilution of 10 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-25186PCP. Image from the standard format of this antibody.")
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