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5'-Nucleotidase/CD73 Antibody (AD2) [CoraFluor™ 1]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC
Clone
AD2
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-48480AF350
Alexa Fluor 405
NBP2-48480AF405
Alexa Fluor 488
NBP2-48480AF488
Alexa Fluor 532
NBP2-48480AF532
Alexa Fluor 594
NBP2-48480AF594
Alexa Fluor 647
NBP2-48480AF647
Alexa Fluor 700
NBP2-48480AF700
Alexa Fluor 750
NBP2-48480AF750
Allophycocyanin
NBP2-48480APC
Biotin
NBP2-48480B
DyLight 350
NBP2-48480UV
DyLight 405
NBP2-48480V
DyLight 488
NBP2-48480G
DyLight 550
NBP2-48480R
DyLight 594
NBP2-48480DL594
DyLight 650
NBP2-48480C
DyLight 680
NBP2-48480FR
DyLight 755
NBP2-48480IR
FITC
NBP2-48480F
HRP
NBP2-48480H
Janelia Fluor 525
NBP2-48480JF525
Janelia Fluor 549
NBP2-48480JF549
Janelia Fluor 585
NBP2-48480JF585
Janelia Fluor 635
NBP2-48480JF635
Janelia Fluor 646
NBP2-48480JF646
Janelia Fluor 669
NBP2-48480JF669
PE
NBP2-48480PE
PerCP
NBP2-48480PCP
Unconjugated
NBP2-48480
mFluor Violet 450 SE
NBP2-48480MFV450
mFluor Violet 500 SE
NBP2-48480MFV500
mFluor Violet 610 SE
NBP2-48480MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-48480
BSA Free
NBP2-48480SS
BSA Free
NBP2-48480
Azide and BSA Free
NBP2-80552
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5'-Nucleotidase/CD73 Antibody (AD2) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Additional Information
Clone AD2 was used by HLDA to establish CD designation.
Immunogen
Human CD73 [UniProt# P21589]
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NT5E
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow (Cell Surface)
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for 5'-Nucleotidase/CD73 Antibody (AD2) [CoraFluor™ 1]

  • 5' nucleotidase (CD73)
  • 5'-NT
  • 5-NT
  • 5'-Nucleotidase
  • 5'-nucleotidase, ecto (CD73)
  • CD73 antigen
  • CD73
  • E5NT
  • EC 3.1.3.5
  • ecto-5'-nucleotidase
  • eN
  • eNT
  • NT
  • NT55'-nucleotidase
  • NT5E
  • NTE
  • Purine 5-Prime-Nucleotidase

Background

Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC 3.1.3.5) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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  1. We are interested in CD73 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
    • We do not currently carry any CD73 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD73 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.

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Bioinformatics

Gene Symbol NT5E